Microbiology: A Systems Approach: Microbiology Quiz 2 js Flashcards


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Microbiology
Grade levels:
College: Second year
Subjects:
science, life sciences, microbiology
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1

what is the abbreviation TGB stand for

Thioglycolate Broth

2

what is the purpose of Thioglycolate Broth?

growth of anaerobic microbe

3

What is the category of Thioglycolate Broth?

Biochemical

4

What is the Inoculation of the Thioglycolate Broth?

Dip and Swirl

5

What does the initials TSB stand for?

Trypticase Soy Broth

6

What is the purpose Trypticase Soy Broth?

growth of wide range of bacteria

7

what is the category for Trypticase Soy Broth?

general

8

what is the inoculation of the Trypticase Soy Broth?

Dip and Swirl

9

What does TSA stand for?

trypticase Soy Agar

10

What is the Purpose of Trypticase Soy Agar?

growth of a wide Range of bacteria.

11

what is the category of Trypticase Soy Agar?

general

12

What is the inoculation of Trypticase Soy Agar?

varies

13

What is the inoculation technique for Trypticase soy Agar?

Quadrant streak method using a mixed culture.

14

Clostridium sporogenes
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anaerobic; gram-negative and gram-positive species of clostridium; this species does not produce neurotoxing but can cause intestinal water loss and hemorrhaging by destroying intestinal epithelial cells produces endospores.

15

MRSA

Methicillin-Resistant Staphylococcus aureaus
--------------------

16

VRSA

Vancomycin-Resistant Staphylococcus aureaus
--------------------

17

Staphylococcus
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gram- positive, cocci in grape-like clusters; salt tolerant

18

(a) Staphylococcus aureaus
---------------------

wide-spread, assoc. with skin and mucus membranes; most resistant of the non-spore formers;drug resistance common, MRSA, VRSA;many virulence factors; food poisoning,toxic shock, scaled skin syndrome, pinkeye,boils etc; most common nosocomial bacterial infection; Lab: MSA- selective and differential; positive coagulase test.

19

(b) Staphylococcus aureaus
--------------------

on the skin; opportunist; most strains produce a slime layer which may enable them to attach to certain hospital apparatuses used in invasive procedures; infections at the site of prosthetic implantation; multiple antibiotic resistance and debilitated patient + severe and frequently fatal infection; MSA selective for organisms

20

(c) Staphylococcus saprophyticus
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opportunistic; a leading cause of Urinary Tract Infection; young women are more susceptible to colonization; hydrolyzes urea to produce ammonia allowing for its growth in an acidic urine environment; spread through sexual intercourse.

21

Escherichia coli
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Gram-negative rod;belongs to the coliform group; common resident of the digestive tract-produces vitamin K and B-complex; "indicator bacterium" the presence of which indicates fecal contamination; opportunist: usual cause of urinary tract infection, also Montezuma's revenge, food poisoning; strain 0157:H7 enterohemorrhagic that produces a toxin: Lab media: EMB and VRB- selective and differential medium: LSB-ID coliforms.

22

Serratia marcescens
----------------

gram-negative motile rod; ubiquitous-soil and water; commonly found growing in bathrooms where it looks pink and slimy; nosocomial; opportunistic in AIDS victims,pneumonia in alcoholics, burn and wound infections; resistant to antibiotics; Lab-at 25 C red pigmented, DNAse,lipase, gelatinase producer.

23

Micrococcus luteus
----------------

gram-positive;aerobic; Cocci found in pairs of clusters; appears yellow in culture; non pathogenic: grows well at 25 c

24

Pseudomonas aeruginosa
----------------------

gram-negative rod; aerobe;motile Pseudomonas isolation agar-selective medium; opportunistic pathogen with a phagocyte-resistant slime layer; nosocomial; fruty odor and a blue green pigment; causes eye, ear and skin infections; also grows at 42 c

25

What does MSA stand for ?

Mannitol Salt Agar

26

What does EMB stand for?

Eosin Methylene Blue Agar