Microbiology Lab Practical

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1

Condenser

Focuses light through specimen

2

Iris Diaphragm

Delivers concentrated beam of light to specimen

3

Coarse Adjustment Knob

For focusing on scanning. When the knob is turned, the stage moves up or down in order to coarse adjust the focus.

4

Fine Adjustment Knob

For precise focusing once initial focusing has been done.

5

Illuminator

Light Source

6

Stage

Holds microscope slide in position

7

Ocular Lens

Remagnifies image framed by objective lens.

8

Objective Lens

4 objective lenses on a microscope, consisting of 4X, 10x, 40x, nd 100x magnification powers. In order to obtain total magnification of an image, multiply eyepiece lens power by objective lens power.

9

Labeled Microscope

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10

If you're looking at something under the microscope and want to move the slide backwards, what would you do?

Move it forward,

11

How to prepare an organism for staining

  1. Smear
  2. Air dry
  3. Heat fix
12

What's a negative stain?

When you stain the background and the organism is clear.

13

When do you do a negative stain?

When you can't heat fix an organism.

14

What color is a gram positive organism?

Purple

15

What color s a gram negative organism?

Red

16

A simple stain is...

Just coloring it.

17

Gram Stain Steps

  1. Crystal Violet
  2. Iodine
  3. Alcohol
  4. Safranin
18

If an acid fast stain is positive it is positive for...

Mycobacterium

19

If you do an endospore stain and find endospores, it is positive for what 2 genuses?

Bacillus and Clostridum.

20

Endospore Stain Procedure

  • Malachite Green
  • Wash with water
  • Safranin

(red rods, green cocci)

21

Acid Fast Stain

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22

Acid fast Pos/Neg

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23

Capsule Stain

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24

Endospore Stain

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25

Blood Agar is selective for...

Streptococcus

26

Blood Agar is differential for...

An organism's ability to hemolyze red blood cells

27

Eosin Methylene Blue (EMB) Agar is selective for...

Members of enteric bacteria (Gram negative rods)

28

Eosin Methylene Blue (EMB) Agar is differential for...

An organism's ability to ferment lactose.

29

Color of nonpathogen on EMB Agar?

Green

30

Color of weak pathogen on EMB Agar?

Pink

31

Color of strong pathogen on EMB Agar?

White

32

MacConkeys Agar is selective for....

Enteric gut bacteria

33

MacConkeys Agar is differential for...

An organism's ability to ferment lactose

34

Color of nonpathogen on MAC Agar?

Pink

35

Color of pathogen on MAC Agar?

White

36

Mannitol Salt Agar is selective for...

Staphylococcus aureus

37

Mannitol Salt Agar is differential for...

An organism's ability to ferment the carbohydrate mannitol

38

Color of a pathogen on Mannitol Salt Agar plate?

Yellow

39

Color of nonpathogen on Mannitol Salt Agar plate?

Red

40

Blood Agar Plate Hemolysis

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41

Blood Agar Plate Hemolysis 2

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42

MSA Pos/Neg Colors

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43

MSA organisms that are pos/neg

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44

EMB pos/neg

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45

EMB organisms that are pos/neg

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46

MAC pos/neg

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47

MAC organisms that are pos/neg

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48

What does a positive Oxidase test look like?

Dark blue/purple

49

What does a negative Oxidase test look like?

No color change

50

What is the color indicator in a fermentation test/MSA plate?

Phenol Red

51

What does a positive Catalase test look like?

Bubbles

52

What does a negative Catalase test look like?

No bubbles

53

What does a positive Nitrate Reduction test look like?

Gas (non fermenter), Red color (after addition of reagents A and B), No color change (after addition of Zinc dust.)

54

What does a negative Nitrate Reduction test look like?

No gas, Red color (after addition of Zinc dust.)

55

What gas is produced from a fermentation test?

Carbon Dioxide

56

What does a positive Phenol Red test look like?

Yellow

57

What does a negative Phenol red test look like?

Red

58

What does a positive Methyl Red test look like?

Red

59

What does a negative Methyl Red test look like?

No color change; yellow

60

What does a positive Voges-Proskauer test look like?

Red ring; red

61

What does a negative Voges-Proskauer test look like?

No color change; yellow

62

What does a positive Starch Hydrolysis test look like?

Halo

63

What does a negative Starch Hydrolysis test look like?

No halo

64

What does a positive Citrate test look like?

Blue, or there is no color change but growth is present

65

What does a negative Citrate test look like?

Green, no growth

66

What does a positive Sulfur Reduction test look like?

Black

67

What does a negative Sulfur Reduction test look like?

No black in medium; yellow

68

What does a positive Indole test look like?

Red ring

69

What does a negative Indole test look like?

No color change; yellow

70

What does a positive Motility test look like?

Growth radiating outwards from stab line

71

What does a negative Motility test look like?

No radiating growth

72

What does a positive Gelatinase test look like?

Liquid

73

What does a negative Gelatinase test look like?

Solid

74

What does a positive Urease test look like?

Fuchsia (pink)

75

What does a negative Urease test look like?

Yellow

76

Oxidase test

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77

Catalase test

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78

Nitrate Reduction test

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79

Methyl Red test

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80

Voges-Proskauer test

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81

Phenol Red Fermentation test

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82

Starch Hydrolysis test

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83

Citrate test

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84

Sulfur test

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85

Indole test

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86

Motility test

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87

Geltinase test

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88

Urease test

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89
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  • Fungi
  • Yeast
  • Saccharomyces cerevisiae
90
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  • Fungi
  • Sporagiospore
91
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  • Fungi
  • Zygospore
92
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  • Fungi
  • Conidiospore
  • Penicillum
93
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  • Fungi
  • Apothecium
  • Pezzia cup
94
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  • Fungi
  • Corpinus sp.
  • Mushroom
95
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  • Lichen
96
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  • Protozoa
  • Amoeba
97
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  • Protozoa
  • Paramecium
98
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  • Protzoa
  • Euglena
99
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  • Protozoa
  • Trichomas
100
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  • Protozoa
  • Trypanosoma
101
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  • Protozoa
  • Plasmodium
102
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  • Trematodes
  • Asian Liver Fluke
103
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  • Cestodes tapeworm
  • Scolex
104
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  • Cestodes tapeworm
  • Proglottids
105
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  • Nematodes
  • Enterobius vermicularis Adult
106
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  • Nematodes
  • Necator Americanus larvae
107

UV light works by Inducting genetic mutations called...

Thymine Dimmers

108

What kind of Agar does the Kirby-Bauer test use?

Mueller-Hinton

109

What do you measure in a Kirby-Bauer test?

Zone of inhibition

110

What do we measure the Zone of Inhibition in?

Millimeters

111

What does the Membrane Filter Technique use?

Eosin Methylene Blue agar (EMB) in combination with a membrane filter

112

When is water considered potable in the Membrane Filter Technique?

If the count is less than 1 coliform per 100 mLs.

113

What color is Methylene blue when it is oxidized?

Blue

114

What color is Methylene blue when it is reduced?

Colorless

115

What is an indicator of good quality milk in a Methylene Blue Reductase test?

It takes more than 6 hours to turn from blue to white.

116

What is the pH indicator in the Snyder test?

Bromcresol blue

117

What does a positive Snyder test look like?

Yellow

118

What does a negative Snyder test look like?

Green

119

The Snyder test is a test to detect...

The prescience of Lactobacillus in saliva as an indicator of dental care susceptbility.

120

Methylene Blue test

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121

Snyder test

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Positive is yellow

122

What does color change in less than 24 hours mean on a Snyder test?

High susceptibility

123

What does color change in less than 48 hours mean on a Snyder test?

Moderate susceptibility

124

What does no color change 72 hours mean on a Snyder Test?

Negative result or low susceptibility.

125

The Oxidase test differential for

Enterobacteriales and Pseudomonas

126

Phenol red differentiates between ______________ and _____________

E.Coli and Proteus vulgaris

127

MR/VP differential for

E.Coli and Enterobacter aerogenes

128

Catalase test differential for

Staphylococcus areus and Streptococcus

129

Nitrate Reduction differentiates for

Pseudomonas, Bacillus subtitles, e. coli

130

Citrate test differential for

E.Coli and Enterobacter aerogenes

131

Starch Hydrolysis differential for

Bacillus subtitles, pseudomonas arguenosa, e.coli

132

Urease differential for

Proteus and Enterobacter

133

Gelatinase differential for

E.Coli and Proteus

134

Sulfur differential for

E.Coli, Salmonella, Shigella

135

Indole differential for

E.Coli, Salmonella, Shigella

136

Motility differential for

E.Coli, Salmonella, Shigella