Microbiology: Microbiology Lab Quiz 2 Flashcards


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1

What is the purpose of doing an acid fast stain?

It is a differential stain used to detect cells capable of retaining a primary stain when treated with acid alcohol.

2

What bacteria is an acid fast stain used to identify?

Mycobacterium

3

What are the 3 primary steps involved in doing an acid fast stain?

  1. Stain with carbolfuchsin
  2. Decolorize with alcohol
  3. Counterstain with methylene blue
4

What three organisms would be positive for a capsule stain?

Bacillus anthracis, Streptococcus pneumoniae, Klebsiella pneumoniae

5

What is a capsule stain?

A differential stain which selectively stains external capsules surrounding bacterial cells.

6

What is the purpose of doing an endospore stain?

To detect the presence, shape, and location of endospores in bacterial cells.

7

What are the two main genera identified by the presence of endospores?

  1. Bacillus
  2. Clostridum
8

What is the procedure for endospore stain?

  1. Stain with malachite green
  2. Heat fix
  3. Decolorize with water
  4. Counterstain with safranin
9

Blood Agar is selective for...

Streptococcus

10

Blood Agar is differential for...

An organism's ability to hemolyze red blood cells

11

Blood Agar: Alpha hemolysis

Mild or incomplete hemolysis

12

Blood Agar: Beta hemolysis

Complete hemolysis

13

Blood Agar: Gamma Hemolysis

No hemolysis

14

Mannitol Salt Agar is selective for...

Staphylococcus aureus

15

Mannitol Salt Agar is differential for...

An organism's ability to ferment the carbohydrate mannitol.

16

Color of a pathogen on Mannitol Salt Agar plate?

Yellow

17

Color of nonpathogen on Mannitol Salt Agar plate?

Red

18

Eosin Methylene Blue (EMB) Agar is selective for...

Members of enteric bacteria (Gram negative rods)

19

Eosin Methylene Blue (EMB) Agar is differential for...

An organism's ability to ferment lactose.

20

Color of nonpathogen on EMB Agar?

Green

21

Color of weak pathogen on EMB Agar?

Pink

22

Color of strong pathogen on EMB Agar?

White

23

MacConkeys Agar is selective for....

Enteric gut bacteria

24

MacConkeys Agar is differential for...

An organism's ability to ferment lactose

25

Color of nonpathogen on MAC Agar?

Pink

26

Color of pathogen on MAC Agar?

White

27

Selective Media

Suppresses the growth of some organisms and promotes the growth of others (Gram + or Gram -)

28

Differential Media

Allows you to differentiate between two types of bacteria (fermentation of lactose.)