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Microbiology Lab Quiz 2

front 1

What is the purpose of doing an acid fast stain?

back 1

It is a differential stain used to detect cells capable of retaining a primary stain when treated with acid alcohol.

front 2

What bacteria is an acid fast stain used to identify?

back 2

Mycobacterium

front 3

What are the 3 primary steps involved in doing an acid fast stain?

back 3

  1. Stain with carbolfuchsin
  2. Decolorize with alcohol
  3. Counterstain with methylene blue

front 4

What three organisms would be positive for a capsule stain?

back 4

Bacillus anthracis, Streptococcus pneumoniae, Klebsiella pneumoniae

front 5

What is a capsule stain?

back 5

A differential stain which selectively stains external capsules surrounding bacterial cells.

front 6

What is the purpose of doing an endospore stain?

back 6

To detect the presence, shape, and location of endospores in bacterial cells.

front 7

What are the two main genera identified by the presence of endospores?

back 7

  1. Bacillus
  2. Clostridum

front 8

What is the procedure for endospore stain?

back 8

  1. Stain with malachite green
  2. Heat fix
  3. Decolorize with water
  4. Counterstain with safranin

front 9

Blood Agar is selective for...

back 9

Streptococcus

front 10

Blood Agar is differential for...

back 10

An organism's ability to hemolyze red blood cells

front 11

Blood Agar: Alpha hemolysis

back 11

Mild or incomplete hemolysis

front 12

Blood Agar: Beta hemolysis

back 12

Complete hemolysis

front 13

Blood Agar: Gamma Hemolysis

back 13

No hemolysis

front 14

Mannitol Salt Agar is selective for...

back 14

Staphylococcus aureus

front 15

Mannitol Salt Agar is differential for...

back 15

An organism's ability to ferment the carbohydrate mannitol.

front 16

Color of a pathogen on Mannitol Salt Agar plate?

back 16

Yellow

front 17

Color of nonpathogen on Mannitol Salt Agar plate?

back 17

Red

front 18

Eosin Methylene Blue (EMB) Agar is selective for...

back 18

Members of enteric bacteria (Gram negative rods)

front 19

Eosin Methylene Blue (EMB) Agar is differential for...

back 19

An organism's ability to ferment lactose.

front 20

Color of nonpathogen on EMB Agar?

back 20

Green

front 21

Color of weak pathogen on EMB Agar?

back 21

Pink

front 22

Color of strong pathogen on EMB Agar?

back 22

White

front 23

MacConkeys Agar is selective for....

back 23

Enteric gut bacteria

front 24

MacConkeys Agar is differential for...

back 24

An organism's ability to ferment lactose

front 25

Color of nonpathogen on MAC Agar?

back 25

Pink

front 26

Color of pathogen on MAC Agar?

back 26

White

front 27

Selective Media

back 27

Suppresses the growth of some organisms and promotes the growth of others (Gram + or Gram -)

front 28

Differential Media

back 28

Allows you to differentiate between two types of bacteria (fermentation of lactose.)