Summer Immuno Lecture 4 Flashcards

APCs use two major “billboards” to display peptide fragments. Which are they?
A. IgM and IgG
B. C3b and C5b
C. Class I and II MHC
D. CD40 and CD40L

Class I MHC has what type of peptide-binding groove?

______ at both ends

Class II MHC has what type of peptide-binding groove?

______ at both ends

Because class I MHC has a closed groove, what peptide length fits best?
A. 3–5 amino acids
B. 8–9 amino acids
C. 13–25 amino acids
D. 30–40 amino acids

Because class II MHC has an open groove, what peptide length can it fit?
A. 4–6 amino acids
B. 8–9 amino acids
C. 10–12 amino acids
D. 13–25 amino acids

Every human has three genes encoding class I HLA proteins. Which set is correct?

The class I HLA genes are located on which chromosome?

Each class I HLA protein pairs with which protein?
A. β2-microglobulin
B. TAP 1
C. TAP 2
D. Proteasome

HLA protein plus β2-microglobulin forms a complete what?
A. Class II MHC
B. Complement receptor
C. Class I MHC
D. B-cell receptor

Humans have the same gene for which class I partner protein?
A. HLA-A
B. HLA-B
C. HLA-C
D. β2-microglobulin

Class I HLA genes are best described as what?

______ polymorphic

Class I MHC is selective about amino acids located where?

Peptide ______

Class I MHC is relatively “promiscuous” about amino acids located where?
Peptide ______

Class II MHC molecules are encoded by which HLA region?
A. HLA-A region
B. HLA-B region
C. HLA-D region
D. HLA-C region

The HLA-D region is located on which chromosome?

Compared with class I, class II MHC has selective binding points located where?
A. Only at ends
B. Along the groove
C. Only on β2-microglobulin
D. Outside the groove

MHC I molecules display what material?
A. Fragments of proteins
B. Whole antibodies
C. Intact bacteria
D. Free carbohydrates

Almost every nucleated cell expresses which MHC class?

Class I MHC molecules are inspected mainly by which cells?
A. Helper T cells
B. Plasma cells
C. B cells
D. Killer T cells

Killer T cells are also called what?
A. Plasma lymphocytes
B. Cytotoxic lymphocytes
C. Regulatory lymphocytes
D. Follicular lymphocytes

Cytotoxic T lymphocytes inspect class I MHC mainly to determine whether a cell is what?
A. Infected
B. Opsonized
C. Allergic
D. Complement-coated

Approximately how many class I MHC molecules are on each cell?
A. 100,000
B. 1,000
C. 10 million
D. 60

Class I MHC molecules on a cell are replaced how often?
A. Hourly
B. Weekly
C. Daily
D. Monthly

Old or defective intracellular proteins are broken down by what?
A. Lysosomes
B. Ribosomes
C. Golgi bodies
D. Proteasomes

Protein fragments for class I loading are transported into which organelle?

_____ _____

Which transporters carry fragments into the ER?

_____ 1 and _____ 2

Some macrophages cut proteins into segments specifically for which transporters?
A. HLA transporters
B. C1 transporters
C. CD40 transporters
D. TAP transporters

Macrophages can process proteins specifically for TAP and which molecule?
A. Class II MHC
B. β2-microglobulin alone
C. Class I MHC
D. Complement receptors

A viral protein is synthesized inside an epithelial cell. Which MHC class displays its fragments?

A bacterial protein is engulfed by an APC from outside the cell. Which MHC class is generally suited for longer peptides?

A class I peptide must fit tightly at the groove ends. What explains this?
A. Closed binding groove
B. Open binding groove
C. HLA-D encoding
D. C1 binding

A class II peptide can extend beyond the groove. What explains this?
A. β2-microglobulin pairing
B. TAP transport
C. HLA-C polymorphism
D. Open binding groove

Which molecule is required to complete a class I MHC molecule?
A. C3b
B. CD40L
C. β2-microglobulin
D. Igβ

Which molecule is not encoded by polymorphic class I HLA genes?
A. β2-microglobulin
B. HLA-A
C. HLA-B
D. HLA-C

Which sequence best fits MHC I loading?
A. ER → proteasome → TAP
B. Proteasome → TAP → ER
C. TAP → lysosome → CD40
D. C1 → TAP → membrane

A macrophage receives a cytokine signal that changes its proteasome composition for better MHC I presentation. Which cytokine caused this?

IFN-γ upregulates which proteasome-associated components?
A. LMP2, LMP7, MECL1
B. TAP1, TAP2, CLIP
C. B7, CD28, CD40
D. HLA-A, HLA-B, C1

LMP2, LMP7, and MECL1 fit into which structure?

IFN-γ-modified proteasomes preferentially cut proteins after which amino acids?
A. Acidic or polar
B. Hydrophobic or basic
C. Aromatic or acidic
D. Neutral or sulfur-containing

Proteasomes cut after hydrophobic/basic residues because TAP and MHC I favor these where?
A. Peptide C-termini
B. Peptide N-termini
C. MHC II grooves
D. Invariant chains

TAP transporters preferentially bind peptides of what length?
A. 3–5 amino acids
B. 6–7 amino acids
C. 20–30 amino acids
D. 8–16 amino acids

Most proteins degraded by proteasomes are best described as what?
A. Extracellular proteins
B. Structurally flawed proteins
C. Complement-coated proteins
D. Antibody-bound proteins

Proteasomal degradation usually targets flawed proteins more than simply what?
A. Viral
B. Exogenous
C. Old
D. Secreted

Class II MHC molecules are expressed exclusively on which cells?
A. Red blood cells
B. Immune system cells
C. Skeletal muscle cells
D. Endothelial cells only

Class II MHC molecules are designed to present antigen to which cells?

Class II MHC molecules are made from which two protein chains?
A. Heavy and light
B. CD40 and CD40L
C. TAP1 and TAP2
D. α and β

In the ER, class II MHC α and β chains bind which third protein?

_____ chain

Class II MHC binds the invariant chain in which organelle?

_____ _____

The invariant chain (Ii) serves as a chaperone for MHC Class II molecules. During biosynthesis in the _____ _____ , it occupies the _____ -binding groove of the MHC II molecule, acting as a placeholder to prevent _____ peptide loading

The invariant chain helps guide MHC II molecules through which structure?

____ ____

After the Golgi, invariant-chain-bound MHC II is guided to which vesicles?

____

The goal of MHC II presentation is to display which proteins?
A. Endogenous proteins
B. Exogenous proteins
C. Structurally flawed proteins
D. Cytosolic proteins

Once inside an endosome, MHC II merges with what structure?

_____

The phagosome carries what material into the MHC II-loading pathway?
A. TAP transporters
B. Outside protein debris
C. β2-microglobulin
D. CD28 molecules

Inside the endosome, exogenous proteins are what?
A. Secreted intact
B. Sent to TAP
C. Bound by C1
D. Broken down

During endosomal processing, most invariant chain is destroyed except what piece?
A. CLIP
B. TAP1
C. B7
D. LMP7

CLIP remains temporarily in the MHC II groove to do what?

____ the groove

Which protein releases CLIP from MHC II?

HLA-DM release of CLIP allows loading of what?
A. Endogenous protein fragment
B. Exogenous protein fragment
C. Whole bacterium
D. C1 complex

HLA-DM also helps ensure MHC II presents peptides that fit how?

HLA-DM competes with peptides trying to bind which molecule?
A. MHC II
B. MHC I
C. CD28
D. TAP1

HLA-DM acts like a peptide editor by favoring what?
A. Fastest peptides
B. Largest proteins
C. Tightest binders
D. Complement fragments

For T cells to function, they need cognate antigen plus what?
A. Complement fixation
B. Antibody secretion
C. TAP transport
D. Co-stimulation

Killer T cells and helper T cells both require antigen presented by what?
A. MHC molecules
B. Antibodies
C. Complement proteins
D. Fc receptors

Which cells have both class I and class II MHC and provide co-stimulation?

Co-stimulation usually involves B7 on which cell?
A. T cell
B. NK cell
C. APC
D. Plasma cell

Co-stimulation usually involves CD28 on which cell?
A. T cell
B. APC
C. Macrophage
D. Bacterium

A T cell recognizes antigen-MHC but receives no co-stimulation. What key signal is missing?
A. Antigen signal
B. TAP signal
C. CLIP signal
D. B7-CD28 signal

Which sequence best fits MHC II loading?
A. Proteasome → TAP → ER
B. C1 → C3 → C5
C. CD28 → B7 → CLIP
D. ER → Golgi → endosome

IFN-γ-induced LMP proteins help generate peptides suited for which transporters?
A. HLA-DM
B. B7
C. TAP
D. CD28

IFN-γ-induced proteasome changes help generate peptides suited for which MHC class?
A. MHC II
B. Secretory IgA
C. Complement C1
D. MHC I

TAP favors peptides with hydrophobic/basic features at which end?
A. N-terminus
B. C-terminus
C. Middle only
D. Both ends equally

MHC I favors hydrophobic/basic features at which end?
A. C-terminus
B. N-terminus
C. CLIP region
D. Invariant chain

Which set correctly lists the three main APC types?
A. Neutrophils, eosinophils, basophils
B. NK cells, mast cells, platelets
C. Dendritic cells, macrophages, B cells
D. Erythrocytes, monocytes, plasma cells

For a macrophage to function well as an APC, it must usually be what?

Which B-cell state can function as an APC?

Dendritic cells are classically described as having what shape?
A. Spherical
B. Starfish-like
C. Spindle-shaped
D. Biconcave

A dendritic cell migrates to a lymph node and activates a never-before-stimulated T cell. Which T cell is this?
A. Experienced T cell
B. Memory T cell
C. Virgin T cell
D. Regulatory T cell

Which APC is especially important for initiating immune responses?

Resting dendritic cells act as sentinel cells beneath which barrier?

Resting dendritic cells beneath epithelial barriers are best described as what?
A. Antibody factories
B. Sentinel cells
C. Cytotoxic lymphocytes
D. Complement proteins

Resting dendritic cells store many reserve molecules of which class?
A. Class I MHC
B. IgG
C. CD1
D. Class II MHC

Upon activation, reserve class II MHC molecules are loaded with what?
A. Surrounding antigens
B. Nuclear DNA only
C. Complement inhibitors
D. IgE antibodies

A dendritic cell loads nearby antigens as it activates. This is like taking what?
A. Blood sample
B. Complement pulse
C. Local snapshot
D. Thymic census

After activation, dendritic cells upregulate production of which class?
A. Class II MHC
B. CD1
C. IgA
D. Class I MHC

Once activated, a dendritic cell travels through which system?

Activated dendritic cells increase production of which co-stimulatory protein?

The activated dendritic cell’s destination is usually which site?

By the time an activated dendritic cell reaches a lymph node, it can activate which cells?
A. Naive B cells only
B. Virgin T cells
C. Erythrocytes
D. Neutrophils

How long does an activated dendritic cell live after reaching a lymph node?
A. About 1 week
B. About 1 day
C. About 1 month
D. About 1 year

As activated dendritic cells leave tissues, they produce what signaling molecules?
A. Antibodies
B. Complement pores
C. Histamine granules
D. Chemokines

Dendritic-cell chemokines recruit which cells into tissues?
A. Plasma cells
B. Erythrocytes
C. Monocytes
D. Platelets

Recruited monocytes can enter tissues and become which cells?
A. Dendritic cells
B. Eosinophils
C. Mast cells
D. Plasma cells

Why do activated dendritic cells recruit monocytes as replacements?
A. To kill antibodies
B. To remove lymph nodes
C. To stop antigen capture
D. To obtain fresh snapshots

Dendritic antigen-presenting cells generally do not what?
A. Present antigen
B. Express MHC
C. Activate T cells
D. Kill

Which APC distinction is correct?
A. Dendritic cells kill
B. Macrophages do not travel
C. B cells lack receptors
D. APCs lack MHC

Activated macrophages mainly provide consistent restimulation of which T cells?
A. Virgin T cells
B. Naive T cells
C. Experienced T cells
D. Immature T cells

Activated macrophages restimulate T cells mainly at what location?
A. Bone marrow
B. Site of infection
C. Thymic cortex
D. Blood plasma

Which APC has an advantage in concentrating antigen?
A. Activated B cell
B. Activated macrophage
C. Dendritic cell
D. NK cell

Activated B cells can concentrate antigen using which receptor?

Experienced B cells act as APCs specifically for which T cells?

Some APCs present exogenous antigen on class I MHC. What is this called?
A. Class switching
B. Cross-presentation
C. Affinity maturation
D. Polyclonal activation

Cross-presentation uses exogenous antigens for which MHC class?
A. Class II only
B. CD1 only
C. Secretory IgA
D. Class I MHC

Cross-presentation is especially important because exogenous antigens can activate which cells?
A. Plasma cells
B. Mast cells
C. Killer T cells
D. Erythrocytes

The nonclassical MHC family that binds lipids is called what?

CD1 proteins are structurally similar to MHC I because they include what?
A. Two equal chains
B. Invariant chain only
C. Long heavy chain
D. Antibody light chain

CD1 proteins pair with which molecule?
A. CD40L
B. β2-microglobulin
C. CLIP
D. C3b

CD1 proteins are part of which MHC family?
A. Classical MHC
B. Antibody MHC
C. Nonclassical MHC
D. Complement MHC

Organ transplant rejection is primarily caused by recognition of what?
A. MHC molecules
B. IgA molecules
C. C1 inhibitors
D. Histamine

Resting dendritic cells load reserve class II MHC molecules when they become what?
A. Degranulated
B. Activated
C. Apoptotic
D. Anucleate

Steps of T cell activation
1. First must recognize _____ _____ presented by MHC
2. recieve _____ signal from APC

Which cell produces large amount of interferon Alpha and beta during viral attack?

Major histocompatibility complex is located on chromsome ____

B cells rely on specific, membrane-bound antibodies (B Cell Receptors) that require ______ by the presented antigen.