Lab Exam 1 Bio 1407 Flashcards


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1

Rules of Lab Safety

No food or drink
Dont touch your eyes or mouth
Wear lab attire
Clean your station
Report Accidnets
Buddy System
Respect Lab equipment
WASH YOUR HANDS

2

Steps of Scientific Method

Observation, Ask a question, Hypothesis, Prediction, experiment, analyze, share/re experiment

3

Experimental design: idependent vs dependendent variable

Independent what you manipulate
dependents - what changes because of the independent

4

Control vs Experimental

Control does not receive the experiment/independent variable
Experimental DOES receive the experiment/independent variable

5

Quantitative vs Qualitative

Quantitative - numbers based and measured
Qualitative - interpretation based and descriptive

6

Data Analysis

what patterns do you see, what relationships do you see

7

Kinds of graphs

bar, line, pie

8

graphs need to have

descriptive title, axis labels with units, key or legend if applicable

9

definition of natural selection and evolution

natural selection is one mechanism of evolution with differential survival and reproduction that results in adaptations

evolution is change in allele frequency over time

10

Selective pressure

external agents which affect an organisms ability to survive in a given environment.

11

Simulation of natural selection in a population

(darwin finches lab, this is not actually a flash card, just a reminder)

12

Categories of evidence for evolution

fossils, homology, biogeography, embryology, microbiology, genetics

13

parts of a phylogenetic tree

root, branch tips, nodes, sister taxa, (ancestral vs derived traits)

14

building trees using evidence

(lab reminder, another card that is not a card)

15

population genetics

Study of how selective forces change
the allele frequencies in a population
over time

16

founder effect

where a very small population starts a new population, having limited genetic diversity (amish)

17

bottleneck effect

an external force severely decreases the population, resulting in limited genetic diversity (see XP in Dine peoples after The Long Walk) random event as well

18

Allele Fixation

a rare case in which a single allele becomes the only one present in the population

19

hardy weinberg in a population

will never happen, a large population with NO evolutionary factors occuring

20

requirements of hardy weinbergy

large population
no mutation
no predation
no migration
no non-random mating

21

HWB equation

p+q=1
p^2 + 2pq +q^2

22

types of selection

directional, stabilizing, disruptive, frequency based

23

Ecomorph

where organisms in similar ecosystems devlop similar traits

24

lamellae

the number of scales on the underside of a lizard's toe to help them grip a stick

25

hindlimb length

hindlimb divided by body length

26

Anolis phylogenetic tree conclusions

each of the ecomorphs evolved separately on their own islands from single ancestors

27

convergent evolution

when two organisms from different ancestors converge on the same trait

28

divergent evolution

two species from the same ancestor become more distinct

29

General structure of viruses

viruses have a capsid as their outer layer, and those that bud from their host will have an 'envelope' , specialized to a host, have DNA but the structure of the DNA can vary, do not reproduce, instead duplicate

30

transmission of viruses

viruses can pass from host to host via things like sneezing or sexually, they can also travel through a vector like an insect, and some can cross species (zoonotic)

31

viral infections

Virus binds to receptor
Virus releases its RNA genome into the cell
The virus's RNA is translated into proteins by ribosomes
Viral RNA polymerase helps transcribe more copies of virus RNA
New viruses travel t othe cell membrane of the infected cell and are released outside the cell

32

Function of anti-virals

to disrupt the reproduction process of the virus by interrupting the translation in the ribosome

33

Covid 19 tests

PCR tests - look for pieces of virus RNA
Antigen - looks for viral spike
Antibody - looks for the body's immune response

34

Calculate vaccine efficacy

divide the infection rate of the experimental group by the infection rate of the control group to get the percentage for efficacy

35

adaptations

traits that increase the survival and reproduction of members in a population

36

Hardy Weinberg Equilibrium

unchanging allele frequencies

37

relative fitness

is the the survival rate of a geno/pheno type relative to the maximum survival or reproduction rate of other genotypes in a population. A number between 0 and 1 (with 1 being the highest fitness)

38

relative tail length

tail length divided by body length

39

how to determine if yogurt has gone bad

bubbles near the bottom and whey and water near the top

40

process of gram stain

heat fix the sample to the slide
add purple stain for 60 sec, rinse
add iodine for 60 sec, rinse
add alcohol for NO MORE THAN 30 SEC, rinse
Add safranin (pink) to stain any gram negative bacteria 60 seconds, rinse

41

Review microscope parts from prokaryotes lab PT 2

review the table :)

42

How do you focus a microscope, how do you put it away

first use coarse adjustment knob to raise and lower the platform, then use the fine adjustment knob to sharpen the focus

remove slide, clean oil off if used, turn off, unplug, place cover on, put away in appropriate spot

43

Bacterial Shapes

cocci/round, bacilli/rod, spiral /spirilla, spirochaetes/corkscrew

44

Why is cyanobacteria incorrect?

Its an algae, not a bacteria

45

Why is the water fern Azolla planted in Rice paddies

symbiotic relationship with cyanobacteria, bacteria provide nitrogen for the ferns

46

What are the problems with cyanobacteria blooms?

can produce chemicals that can poison people and animals, and can devastate eco systems which will also cause economic problems and reduce tourism

47

Be able to indentify Nostoc bacteria from a slide

chain-like green wormy lookin boy

48

what are archaea

prokaryotic organisms often found in extreme environments, distinct from bacteria

49

what are protists

single or multi-cellular Eukaryotes that can be plant, animal, or fungi like. Its a catch all box for organisms that defy organization into other categories, is paraphyletic

50

Methods of Protist motion

psuedopodia (foot like), flagella (like an appendage), cilia (hair like), (or amoeba like)

51

Be able to identify slides of the following:
Diatoms
Trypanosoma
Spirogyra
Vovox
Salpingoeca
Trihonympha

See lab background handout for slides
Diatoms (two plates like a petri dish, sometimes ladder like or with many ridges)
Trypanosoma (looks kinda like a spanish dancer flat worm, has a flagella)
Spirogyra (spiral shaped chloroplast)
volvox (colonial round, flagella, green - each inner orb is also round)
Salpingoeca (happy little alien baby with collar and flagella)
trichonympha (like a weird combo of jellyfish, looks like a gross ice cream cone with cillia)

52

Know all the parts to a fungus

hyphae, mycellium, fruiting body, spores (or zygotes)

53

Know all the parts to a mushroom

cap, gills, ring, stem, cup, mycellium

54

What kind of Troph are fungi

heterotroph, specifically saprobes for decomposers, or parasites for parasite species

55

Phyla names and general characteristics

Ascomyota (sac fungi) yeast/pennicillin
Zygomycota (conjugation fungi) bread mold
Basidiomycota (club and shelf) traditional mushrooms
Chytridiomycota (only fungus with flagella) BD frog fungus
Glomeromycota (symbionts with trees/plants)

56

How does chyrtidio fungus kill frogs

damages skin which damages respiratory system

57

Be able to identify the following through slides

Rhizopus (large spores on the end of stems)
Penicillium (like tiny spores on the end of oval)
Sacchoarmyces ceviseae (like grains of rice, some with hyphae)
Amanita phalloides ( white stem with yellowish top, collar near the top, large volva)
Allomyces (black eyed pea with long flagella)
Lichen cross section (algae layer, mycellium, lower cortex)

58

Rhizopus

large spores on the end of stems, sporangiospores and zygospores, is bread mold

59

penicillum

looks tiny spores on the end of an oval, sac like reproductive structure called ascus, is ascomycota

60

sacchoramyces

looks like rice, is yeast

61

amanita phalloides

death cap mushroom, white with yellow top

62

allomyces

flagellated zoospores, looks like a black eyed pea with a long flagella