Rules of Lab Safety
No food or drink
Dont touch your eyes or mouth
Wear lab
attire
Clean your station
Report Accidnets
Buddy
System
Respect Lab equipment
WASH YOUR HANDS
Steps of Scientific Method
Observation, Ask a question, Hypothesis, Prediction, experiment, analyze, share/re experiment
Experimental design: idependent vs dependendent variable
Independent what you manipulate
dependents - what changes
because of the independent
Control vs Experimental
Control does not receive the experiment/independent
variable
Experimental DOES receive the experiment/independent variable
Quantitative vs Qualitative
Quantitative - numbers based and measured
Qualitative -
interpretation based and descriptive
Data Analysis
what patterns do you see, what relationships do you see
Kinds of graphs
bar, line, pie
graphs need to have
descriptive title, axis labels with units, key or legend if applicable
definition of natural selection and evolution
natural selection is one mechanism of evolution with differential
survival and reproduction that results in adaptations
evolution is change in allele frequency over time
Selective pressure
external agents which affect an organisms ability to survive in a given environment.
Simulation of natural selection in a population
(darwin finches lab, this is not actually a flash card, just a reminder)
Categories of evidence for evolution
fossils, homology, biogeography, embryology, microbiology, genetics
parts of a phylogenetic tree
root, branch tips, nodes, sister taxa, (ancestral vs derived traits)
building trees using evidence
(lab reminder, another card that is not a card)
population genetics
Study of how selective forces change
the allele frequencies in a
population
over time
founder effect
where a very small population starts a new population, having limited genetic diversity (amish)
bottleneck effect
an external force severely decreases the population, resulting in limited genetic diversity (see XP in Dine peoples after The Long Walk) random event as well
Allele Fixation
a rare case in which a single allele becomes the only one present in the population
hardy weinberg in a population
will never happen, a large population with NO evolutionary factors occuring
requirements of hardy weinbergy
large population
no mutation
no predation
no
migration
no non-random mating
HWB equation
p+q=1
p^2 + 2pq +q^2
types of selection
directional, stabilizing, disruptive, frequency based
Ecomorph
where organisms in similar ecosystems devlop similar traits
lamellae
the number of scales on the underside of a lizard's toe to help them grip a stick
hindlimb length
hindlimb divided by body length
Anolis phylogenetic tree conclusions
each of the ecomorphs evolved separately on their own islands from single ancestors
convergent evolution
when two organisms from different ancestors converge on the same trait
divergent evolution
two species from the same ancestor become more distinct
General structure of viruses
viruses have a capsid as their outer layer, and those that bud from their host will have an 'envelope' , specialized to a host, have DNA but the structure of the DNA can vary, do not reproduce, instead duplicate
transmission of viruses
viruses can pass from host to host via things like sneezing or sexually, they can also travel through a vector like an insect, and some can cross species (zoonotic)
viral infections
Virus binds to receptor
Virus releases its RNA genome into the
cell
The virus's RNA is translated into proteins by
ribosomes
Viral RNA polymerase helps transcribe more copies of
virus RNA
New viruses travel t othe cell membrane of the infected
cell and are released outside the cell
Function of anti-virals
to disrupt the reproduction process of the virus by interrupting the translation in the ribosome
Covid 19 tests
PCR tests - look for pieces of virus RNA
Antigen - looks for
viral spike
Antibody - looks for the body's immune response
Calculate vaccine efficacy
divide the infection rate of the experimental group by the infection rate of the control group to get the percentage for efficacy
adaptations
traits that increase the survival and reproduction of members in a population
Hardy Weinberg Equilibrium
unchanging allele frequencies
relative fitness
is the the survival rate of a geno/pheno type relative to the maximum survival or reproduction rate of other genotypes in a population. A number between 0 and 1 (with 1 being the highest fitness)
relative tail length
tail length divided by body length
how to determine if yogurt has gone bad
bubbles near the bottom and whey and water near the top
process of gram stain
heat fix the sample to the slide
add purple stain for 60 sec,
rinse
add iodine for 60 sec, rinse
add alcohol for NO MORE
THAN 30 SEC, rinse
Add safranin (pink) to stain any gram negative
bacteria 60 seconds, rinse
Review microscope parts from prokaryotes lab PT 2
review the table :)
How do you focus a microscope, how do you put it away
first use coarse adjustment knob to raise and lower the platform,
then use the fine adjustment knob to sharpen the focus
remove slide, clean oil off if used, turn off, unplug, place
cover on, put away in appropriate spot
Bacterial Shapes
cocci/round, bacilli/rod, spiral /spirilla, spirochaetes/corkscrew
Why is cyanobacteria incorrect?
Its an algae, not a bacteria
Why is the water fern Azolla planted in Rice paddies
symbiotic relationship with cyanobacteria, bacteria provide nitrogen for the ferns
What are the problems with cyanobacteria blooms?
can produce chemicals that can poison people and animals, and can devastate eco systems which will also cause economic problems and reduce tourism
Be able to indentify Nostoc bacteria from a slide
chain-like green wormy lookin boy
what are archaea
prokaryotic organisms often found in extreme environments, distinct from bacteria
what are protists
single or multi-cellular Eukaryotes that can be plant, animal, or fungi like. Its a catch all box for organisms that defy organization into other categories, is paraphyletic
Methods of Protist motion
psuedopodia (foot like), flagella (like an appendage), cilia (hair like), (or amoeba like)
Be able to identify slides of the following:
Diatoms
Trypanosoma
Spirogyra
Vovox
Salpingoeca
Trihonympha
See lab background handout for slides
Diatoms (two plates like a
petri dish, sometimes ladder like or with many
ridges)
Trypanosoma (looks kinda like a spanish dancer flat worm,
has a flagella)
Spirogyra (spiral shaped chloroplast)
volvox
(colonial round, flagella, green - each inner orb is also
round)
Salpingoeca (happy little alien baby with collar and
flagella)
trichonympha (like a weird combo of jellyfish, looks
like a gross ice cream cone with cillia)
Know all the parts to a fungus
hyphae, mycellium, fruiting body, spores (or zygotes)
Know all the parts to a mushroom
cap, gills, ring, stem, cup, mycellium
What kind of Troph are fungi
heterotroph, specifically saprobes for decomposers, or parasites for parasite species
Phyla names and general characteristics
Ascomyota (sac fungi) yeast/pennicillin
Zygomycota (conjugation
fungi) bread mold
Basidiomycota (club and shelf) traditional
mushrooms
Chytridiomycota (only fungus with flagella) BD frog
fungus
Glomeromycota (symbionts with trees/plants)
How does chyrtidio fungus kill frogs
damages skin which damages respiratory system
Be able to identify the following through slides
Rhizopus (large spores on the end of stems)
Penicillium (like
tiny spores on the end of oval)
Sacchoarmyces ceviseae (like
grains of rice, some with hyphae)
Amanita phalloides ( white stem
with yellowish top, collar near the top, large volva)
Allomyces
(black eyed pea with long flagella)
Lichen cross section (algae
layer, mycellium, lower cortex)
Rhizopus
large spores on the end of stems, sporangiospores and zygospores, is bread mold
penicillum
looks tiny spores on the end of an oval, sac like reproductive structure called ascus, is ascomycota
sacchoramyces
looks like rice, is yeast
amanita phalloides
death cap mushroom, white with yellow top
allomyces
flagellated zoospores, looks like a black eyed pea with a long flagella