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Chapter 7 Microbial Genetics

1.

the transmission of genetic material (DNA and RNA) from an organism to its offspring

heredity

2.

DNA + protein

chromosome

3.

a sequence of DNA that codes for a particular trait

gene

4.

basic unit of heredity

gene

5.

also means location

locus

6.

genes with different information at the same location

alleles

7.

a permanent alternation in DNA

mutation

8.

Deoxyribonucleic Acid

DNA

9.

Ribonucleic Acid

RNA

10.

refers to the genetic information contained in the DNA of the organism

genotype

11.

refers to the specific characteristics displayed by the organism

phenotype

12.

linear sequence of nucleotides of DNA that form a functional unit of a chromosome or a plasmid

gene

13.

information in a specific gene, found at a particular place on the chromosome or plasmid

locus

14.

In what three major ways can the information in DNA be transferred?

1. DNA Replication
2. Transcription
3. Translation

15.

What are the reasons we copy/replicate DNA?

growth
repair
maintenance

16.

In this transferring of information in DNA, DNA produces more DNA

DNA Replication

17.

in this transferring of information in DNA one strand of each DNA molecule is half old and the olther is half new

Semiconservative replication

18.

governs strand assembly and proofreads

DNA polymerase

19.

What do ribosomes make?

proteins

20.

In this part of transferring information in DNA RNA is produced using DNA as a template

transcription

21.

Refers transcription occurs in what?

viruses

22.

forms ribosomes

ribosomal RNA (rRNA)

23.

carries instructions to build proteins to the ribosomes (each three RNA bases specify a particular amino acid or a stop code)

messenger RNA (mRNA)

24.

transfers needed amino acids from cytoplasm to ribosomes

trandfer RNA (tRNA)

25.

Why do ribosomes need amino acids?

to build proteins

26.

in this porption of transferring information within DNA ribosomes "read"messenger RNA and link the coded for amino acids together into proteins

translation

27.

Approximately what percentage of a bacterial cell's energy is used to produce proteins?

80 to 90

28.

What two causes of mutations does your book list?

1. spontaneous
2. induced

29.

mutations that have no know cause

spontaneous

30.

mutations caused by mutagens

induced

31.

tend to increase the mutation rate above the spontaneous rate

mutagens

32.

give two examples of known mutagens

radiation
some chemicals

33.

something that can cause a mutation

mutagen

34.

What are the two tyoes of mutations?

1. Point Mutation
2. Frameshift Mutation

35.

in this type of mutation a single base is replaced and it may or may not cause problems

point mutation

36.

in this type of mutation an insertion or deletion of one or more bases takes place and it always causes a problem

frameshift mutation

37.

these are found in cured meat such as deli meats, hot dogs, and bacon

nitrates and nitrites

38.

cancer causing

carcinogens

39.

one of the first tests done on new products to see if there are mutations

Ames Test

40.

The ames test uses what that can not make histidine

salmonella strain

41.

Why does the ames test use a certain strain of salmonella?

it uses a strain that can not make histidine amino acids so it can't make proteins

42.

What is the end result of the ames test believed to be if the new product does contain mutagens

if salmonella makes histidine right away

43.

removes an amino group (-NH2) from a nitrogenous base to point mutations

deaminating agent

44.

Bacteria have _______ as their genetic material

RNA

45.

Which base is NOT found in DNA?

uracil

46.

The first bacterium to have its genome completely sequenced was:

Haemophilus influenza

47.

The process of reverse transcription creates:

DNA from RNA

48.

During DNA repliction, DNA ploymerase adds nucleotides:

to the 3' end of the growing strand

49.

During DNA replication, the new strand has a sequence ______ to the strand being copied

identical

50.

DNA replication is termed

conservative

51.

The enzyme responsible for transcription is called:

RNA polymerase

52.

In prokaryotes, transcription takes place in the:

ribosomes

53.

Ribosomes are made of

RNA and protein

54.

tRNA is responsible for transporting _______ to the ribosome

amino acid

55.

Each tRNA has a/an _______ that allows it to match up with the correct mRNA codon

DNA sequence

56.

The bond that is created between amino acids is called a _______ bond

ionic

57.

Repressors are molecules that inhibit:

DNA replication

58.

A phenotypoe refers to the ________of an organism

expressed physical characteristics

59.

A mutation that results in a change in DNA sequence, but no change in the amino acid sequence of the protein, is termed ______ mutation

silent

60.

Which of the follwing can induce mutations?

base analogs
ionizinhg radiation
ultraviolent radiation

61.

The Ames Test is used to test whether or not:

a chemical is mutagenic