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Chapter 7 Microbial Genetics

front 1

the transmission of genetic material (DNA and RNA) from an organism to its offspring

back 1

heredity

front 2

DNA + protein

back 2

chromosome

front 3

a sequence of DNA that codes for a particular trait

back 3

gene

front 4

basic unit of heredity

back 4

gene

front 5

also means location

back 5

locus

front 6

genes with different information at the same location

back 6

alleles

front 7

a permanent alternation in DNA

back 7

mutation

front 8

Deoxyribonucleic Acid

back 8

DNA

front 9

Ribonucleic Acid

back 9

RNA

front 10

refers to the genetic information contained in the DNA of the organism

back 10

genotype

front 11

refers to the specific characteristics displayed by the organism

back 11

phenotype

front 12

linear sequence of nucleotides of DNA that form a functional unit of a chromosome or a plasmid

back 12

gene

front 13

information in a specific gene, found at a particular place on the chromosome or plasmid

back 13

locus

front 14

In what three major ways can the information in DNA be transferred?

back 14

1. DNA Replication
2. Transcription
3. Translation

front 15

What are the reasons we copy/replicate DNA?

back 15

growth
repair
maintenance

front 16

In this transferring of information in DNA, DNA produces more DNA

back 16

DNA Replication

front 17

in this transferring of information in DNA one strand of each DNA molecule is half old and the olther is half new

back 17

Semiconservative replication

front 18

governs strand assembly and proofreads

back 18

DNA polymerase

front 19

What do ribosomes make?

back 19

proteins

front 20

In this part of transferring information in DNA RNA is produced using DNA as a template

back 20

transcription

front 21

Refers transcription occurs in what?

back 21

viruses

front 22

forms ribosomes

back 22

ribosomal RNA (rRNA)

front 23

carries instructions to build proteins to the ribosomes (each three RNA bases specify a particular amino acid or a stop code)

back 23

messenger RNA (mRNA)

front 24

transfers needed amino acids from cytoplasm to ribosomes

back 24

trandfer RNA (tRNA)

front 25

Why do ribosomes need amino acids?

back 25

to build proteins

front 26

in this porption of transferring information within DNA ribosomes "read"messenger RNA and link the coded for amino acids together into proteins

back 26

translation

front 27

Approximately what percentage of a bacterial cell's energy is used to produce proteins?

back 27

80 to 90

front 28

What two causes of mutations does your book list?

back 28

1. spontaneous
2. induced

front 29

mutations that have no know cause

back 29

spontaneous

front 30

mutations caused by mutagens

back 30

induced

front 31

tend to increase the mutation rate above the spontaneous rate

back 31

mutagens

front 32

give two examples of known mutagens

back 32

radiation
some chemicals

front 33

something that can cause a mutation

back 33

mutagen

front 34

What are the two tyoes of mutations?

back 34

1. Point Mutation
2. Frameshift Mutation

front 35

in this type of mutation a single base is replaced and it may or may not cause problems

back 35

point mutation

front 36

in this type of mutation an insertion or deletion of one or more bases takes place and it always causes a problem

back 36

frameshift mutation

front 37

these are found in cured meat such as deli meats, hot dogs, and bacon

back 37

nitrates and nitrites

front 38

cancer causing

back 38

carcinogens

front 39

one of the first tests done on new products to see if there are mutations

back 39

Ames Test

front 40

The ames test uses what that can not make histidine

back 40

salmonella strain

front 41

Why does the ames test use a certain strain of salmonella?

back 41

it uses a strain that can not make histidine amino acids so it can't make proteins

front 42

What is the end result of the ames test believed to be if the new product does contain mutagens

back 42

if salmonella makes histidine right away

front 43

removes an amino group (-NH2) from a nitrogenous base to point mutations

back 43

deaminating agent

front 44

Bacteria have _______ as their genetic material

back 44

RNA

front 45

Which base is NOT found in DNA?

back 45

uracil

front 46

The first bacterium to have its genome completely sequenced was:

back 46

Haemophilus influenza

front 47

The process of reverse transcription creates:

back 47

DNA from RNA

front 48

During DNA repliction, DNA ploymerase adds nucleotides:

back 48

to the 3' end of the growing strand

front 49

During DNA replication, the new strand has a sequence ______ to the strand being copied

back 49

identical

front 50

DNA replication is termed

back 50

conservative

front 51

The enzyme responsible for transcription is called:

back 51

RNA polymerase

front 52

In prokaryotes, transcription takes place in the:

back 52

ribosomes

front 53

Ribosomes are made of

back 53

RNA and protein

front 54

tRNA is responsible for transporting _______ to the ribosome

back 54

amino acid

front 55

Each tRNA has a/an _______ that allows it to match up with the correct mRNA codon

back 55

DNA sequence

front 56

The bond that is created between amino acids is called a _______ bond

back 56

ionic

front 57

Repressors are molecules that inhibit:

back 57

DNA replication

front 58

A phenotypoe refers to the ________of an organism

back 58

expressed physical characteristics

front 59

A mutation that results in a change in DNA sequence, but no change in the amino acid sequence of the protein, is termed ______ mutation

back 59

silent

front 60

Which of the follwing can induce mutations?

back 60

base analogs
ionizinhg radiation
ultraviolent radiation

front 61

The Ames Test is used to test whether or not:

back 61

a chemical is mutagenic