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UAB Bio 123 Lab Exam 2

front 1

What do enzymes do?

back 1

Speed up reactions/ catalyze them

front 2

How does temperature affect enzymes?

back 2

If temp is too low, there is not enough kinetic energy, if temp is too high, enzymes denature, Rate of reaction increases as temp increases to a maximum then declines when temperature continues to increase

front 3

Enzymes

back 3

Proteins that speed up or catalyze a reaction

front 4

Active site

back 4

Substrate binding site

front 5

Denaturation

back 5

Breakdown of secondary and tertiary structures of protein

front 6

Competitive inhibitor

back 6

Binds and competes for active site on enzyme

front 7

Non-Competitive Inhibitor

back 7

Impede activity without binding to active site (Changes active site shape)

front 8

Ea (activation energy)

back 8

energy needed to make a reaction occur, catalysts provide this to reactions

front 9

Cofactors

back 9

Usually metallic ions that assist enzymes

front 10

Coenzymes

back 10

Organic cofactors

front 11

Catecholase

back 11

found in fruits and veggies (potatoes), facilitates reaction between catechol and oxygen

front 12

What is catechol converted to?

back 12

Benzoquinone

front 13

How does pH affect enzymes?

back 13

Each enzyme has an optimal pH, if too high or too low enzymes denature

front 14

How does enzyme concentration affect enzymes?

back 14

More enzymes means faster product, with more enzymes means more active sites, more enzymes raise saturation level

front 15

How does substrate concentration affect enzymes?

back 15

When raised the velocity of the reaction will increase until it reaches saturation point (maximum) then remain constant

front 16

Cellular Respiration Formula

back 16

C6H12O6 + 6 O2 --> 6 CO2 + 6 H2O + Energy

front 17

Heterotrophs

back 17

Do not use photosynthesis but obtain food from outside sources

front 18

Facultative anaerobes

back 18

can live in aerobic and anaerobic conditions (Ex: yeast)

front 19

Glycolysis: aerobic or anaerobic?

back 19

Anaerobic

front 20

Where does glycolysis happen?

back 20

In cytoplasm

front 21

What does glycolysis produce?

back 21

4 ATP 2 pyruvate

front 22

Dehydrogenase

back 22

Transfers H+ from substrate to coenzyme

front 23

What coenzymes does glycolysis produce?

back 23

2 NADH

front 24

Where does fermentation happen?

back 24

Cytoplasm

front 25

What is fermentation? What are the types?

back 25

Metabolizes pyruvate when O2 is unavailable, produces 2 ATP, yields alcohol and CO2 or lactic acid

front 26

Why is there iodoform (flaky yellow precipitate) when yeast, NaOH, and lugols are mixed?

back 26

Iodoform forms from interaction with the alcohol because fermentation happened

front 27

What is the transition reaction?

back 27

Happens in mitochondria, converts pyruvate to CoA, makes CO2 as a by-product

front 28

Where does the Kreb Cycle happen?

back 28

Matrix of mitochondria

front 29

Krebs: aerobic or anaerobic?

back 29

Aerobic

front 30

what are Coenzymes of Krebs?

back 30

NADH and FADH2

front 31

what are Products of Krebs?

back 31

1 CoA = 1 ATP, 2 CO2, 3 NADH, & 1 NADPH doubled for complete glucose

front 32

What is used to phosphorylate ADP to ATP?

back 32

Electron transport chain, ATP synthase uses H+ gradient

front 33

Where is the electron transport chain?

back 33

Inner membrane mitochondria

front 34

Cristae

back 34

Inner folds of mitochondria

front 35

H2O

back 35

By-product of electron transport chain

front 36

O2

back 36

Final electron acceptor of electron transport chain

front 37

Photosynthesis equation

back 37

6 CO2 + 6 H2O + light energy --> C6H12O6 + 6 O2

front 38

What does photosynthesis do?

back 38

Captures sun light energy and stores it in chemical bonds of carbohydrates

front 39

Stomata

back 39

Underside of leaves, closes in intense heat

front 40

Xylum

back 40

Water transport system (roots to stem)

front 41

Where does light reaction happen?

back 41

Thylakoids

front 42

Granum

back 42

Stack of thylakoids

front 43

Stroma

back 43

Outside of granum

front 44

Chlorophyll A

back 44

Main chlorophyll pigment

front 45

Chlorophyll B

back 45

More O's in ring structure than other chlorophyll pigment

front 46

Carotenoids

back 46

Accessory pigments for excess light

front 47

Xanthophyll

back 47

Pigment within vacuoles and do not perform photosynthesis

front 48

Light-Dependent Reaction Equation

back 48

H2O + ADP + Pi + NADP+ --> ½ O2 + ATP + NADPH + H+

front 49

Which photosystem phosphorylates ADP?

back 49

Photosystem I

front 50

What splits H2O in photosynthesis?

back 50

light

front 51

Where do the things from noncyclic photosystem II go?

back 51

O2 into atmosphere, H+ to electron transport, electrons to photosystem I

front 52

What wavelength is violet?

back 52

400nm

front 53

What wavelength is blue?

back 53

450nm

front 54

What wavelength is green?

back 54

500nm

front 55

What wavelength is yellow?

back 55

550nm

front 56

What wavelength is orange?

back 56

600nm

front 57

What wavelength is red?

back 57

700nm

front 58

What wavelength is gamma?

back 58

< 1nm

front 59

What wavelength is X-ray?

back 59

<1-100nm

front 60

What wavelength is UV?

back 60

100-350nm

front 61

What wavelength is infrared?

back 61

750- <1 millimeter

front 62

What wavelength are microwave waves?

back 62

<1 millimeter- 1 meter

front 63

What wavelength are radio waves?

back 63

Thousands of meters

front 64

Active spectrum

back 64

relative effectiveness of different wavelengths of light (colors) for light- dependent processes

front 65

Which pigment is heaviest?

back 65

Chlorophyll B

front 66

Which pigment is lightest?

back 66

Carotenoids

front 67

Absorption spectrum

back 67

absorbance vs wavelength graphs

front 68

Dark reaction equation

back 68

6 CO2 + 18 ATP + 12 NADPH + 12 H+ -->C6H12O6 + 18 ADP + 18 Pi + 12 NADP+ + 6 H2O

front 69

What happens in dark reaction?

back 69

Energy is stored by carbon dioxide fixation in carbohydrates

front 70

What is the result of mitosis?

back 70

Two identical daughter cells

front 71

Karyokinesis

back 71

nucleus division

front 72

cytokinesis

back 72

cytoplasm division

front 73

Chromatin

back 73

loosely packaged, not condensed DNA

front 74

Chromatid

back 74

condensed DNA

front 75

Centromeres

back 75

Waist of chromosome where sister chromatids attach

front 76

When does replication happen?

back 76

S phase

front 77

What happens in G2?

back 77

Cells are preparing to divide

front 78

What percent of a cells life is interphase?

back 78

90%

front 79

what are the Three phases of interphase?

back 79

G1, G2, S

front 80

What is the "restriction phase"?

back 80

G1

front 81

G0

back 81

Non dividing phase

front 82

Cell cycle

back 82

Describes life history of actively dividing cells

front 83

What happens in prophase?

back 83

Chromatin condenses, Centrioles go to poles and produce spindle fibers

front 84

Centrosome

back 84

2 centrioles

front 85

What does microtubules form from?

back 85

tubulin

front 86

Where do the spindle fibers attach?

back 86

Kinetochore

front 87

What happens in metaphase?

back 87

Sister chromatids line up at metaphase plate, Attached by spindle fibers to opposite centrioles

front 88

What happens in anaphase?

back 88

Sister chromatids separate at centromeres, Spindle fibers shorten at kinetochore end, Separated chromatids are called daughter chromosomes

front 89

What happens in telophase?

back 89

Spindle apparatus disappears, Nuclei and nucleoli reform, Chromosomes decondense and reform diffuse chromatin, 2 product daughter cells are identical, cytokinesis

front 90

What is cytokinesis? How does this happen in animals? Plants?

back 90

Separation of cytoplasm, cleavage furrow, cell plate

front 91

What does a cell do after mitosis?

back 91

Return to interphase

front 92

What happens to organelles during mitosis?

back 92

ER and golgi break down and are rebuilt, Lysosomes/ ribosomes get split between two cells, Mitochondria and chloroplasts are also split between cells

front 93

What is meiosis?

back 93

The production of sex cells or gametes

front 94

Diploid

back 94

2n

front 95

Haploid

back 95

n

front 96

Spores

back 96

haploid, germinate to form haploid individuals

front 97

What is the product of meiosis?

back 97

4 daughter cells with 1/2 # of chromosomes than parent cell

front 98

Homologous pairs

back 98

identical in size and shape

front 99

Locus

back 99

where genes for a particular trait are found

front 100

Allele

back 100

2 types of one gene

front 101

Homozygous

back 101

When alleles are the same genotype (gene content)

front 102

Heterozygous

back 102

When alleles are different genotypes (gene content)

front 103

Phenotype

back 103

appearance

front 104

Mendel's first law

back 104

states that alleles segregate in meiosis, when two haploid gametes combine during fertilization, two alleles are then present in offspring

front 105

Mendel's second law

back 105

states that alleles of unlinked genes assort independently

front 106

What happens in prophase I?

back 106

Homologous chromosomes come together and synapse, Tetrad forms (2 chromosomes)

front 107

Synaptonemal complex

back 107

Helps pair the homologs

front 108

Where does crossing over happen?

back 108

Chiasmata

front 109

What do centrioles do during Prophase I?

back 109

Centrioles replicated prior to division and move to poles

front 110

When do spindle fibers appear?

back 110

Prophase I

front 111

What happens in metaphase I?

back 111

Homologs line up at metaphase plate

front 112

What happens in anaphase I?

back 112

Homologs separate, Pulled to opposite sides by kinetochore microtubules

front 113

What happens in Telophase I?

back 113

Another centriole division, Cytokinesis, Produces 2 cells

front 114

What happens in Interphase II?

back 114

No replication

front 115

What happens in prophase II?

back 115

Centrioles to poles

front 116

What happens in metaphase II?

back 116

Sister chromatids at metaphase plate

front 117

What happens in anaphase II?

back 117

Sister chromatids loosely associated around centromere region, Sister chromatids are separated

front 118

What happens in telophase II?

back 118

Nuclear membranes form, Cytokinesis, 4 different daughter cells