Aseptic technique Flashcards

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created 7 years ago by Sherae45
updated 7 years ago by Sherae45
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Were all your transfers successful?

Variable answer


How do you know your transfer to a broth was successful?

How do you know your transfers to agar slants were successful?

Success is presence of growth


If any of your transfers were unsuccessful, suggest possible errors that may have been made in the transfer process

Failure is no growth;or growth of a wide variety of colonies, contamination


Provide three reasons why the use of aseptic technique is essential when handling microbial cultures in the laboratory

When handling microbial cultures, aseptic technique limits containation of yours and your workspace with the microbes in the cultures, and it limits contamination of your cultures with unwanted environmental microbes


Provide two examples of how heat is used during inoculation of a tube culture

The flame from a Bunsen burner is used to sterilize transfer instruments (e.g., inoculation loop) and is used to flame the opening of the tube after the cap is removed and before the cap is replaced


How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial sample to/from an agar plate

Since the opening of a plate is not readily flamed, one should hold the lid over the top of the open plate when inoculating so that air contamination is limited. Working near a flame is also useful


Where should a label be written on an agar plate

Labels should be written on the bottom of the agar plate


How should agar plates be incubated? Why?

Agar plates should be incubated in an inverted position to prevent condensation on the agar surface that would spread the inoculated organisms


Disinfectants are effective against which types of organisms? Which types of organisms may remain on the lab bench even after disinfection? What disinfectants is used in your laboratory

Disinfectants, such a bleach and alcohol, are generally useful against vegetative cell and viruses but may not completely eradicate bacterial endospores


Compare and contrast the growth of bacteria in different physical types of media (broths, slants, and agar plates). What might be the advantages and disadvantages of using each type

The presence of growth in a liquid media such as a broth is indicated by turbidity or cloudiness. Individual colonies are not visible and one species could not be separated from another. Solid media such as slants allow for visible growth on the surface that can be observed for color and texture, etc. Plates allow for isolated colony


A disinfectant is used on your work surface

(A) before the beginning of laboratory procedures

(B) after all work is complete

(C) after any spill of live microorganisms


To retrieve a simple from a culture tube with an incoulating loop, the cap of the tube is

Removed and held with the fingers of the loop hand


An inoculating loop or needle is sterilized using heat

Until the entire wire is bright red


Which of the following world be a correctly labeled agar plate?

S. aureus on the bottom


Noah wanted to transfer Staphylococcus aureus from a broth to an agar plate. He picked up the broth culture, removed the cap, and flamed the mouth of the tube. He inserted an inoculating loop to obtain a bacterial sample. Then he flamed the mouth of the tube and replaced the cap. Noah opened the lid of a labeled agar plate diagonally and used the loop to streak the surface of the agar. After closing the lid, he flamed the loop in an incinerator and put it back in its container. The plate was incubated upside down for 24-48 hours. What did Noah do wrong in this transfer

He did not use the transfer tool correctly