Ch. 20 Study Module

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1

What defect causes pituitary dwarfism?

Lack of growth hormone

2

What is genetic cloning?

A method to produce many copies of a gene

3

True or false? The use of growth hormone isolated from cadavers was banned mainly because there were not enough cadavers to supply the hormone.

false

4

Which of the following statements best describes the function of reverse transcriptase?

It makes complementary DNA (cDNA) from mRNA

5

Which of the following steps would be performed last when screening a cDNA library?

Match the region of radioactivity on the filter with the corresponding plate.

6

How can large quantities of protein be produced from a bacterial colony containing the gene

Put a cDNA encoding the protein into a plasmid with a bacterial promoter sequence.

7

True or false? The term "sticky ends" refers to the overhanging ends on DNA that are generated by restriction enzymes, which can base pair with any DNA molecules that contain complementary sticky ends.

True

8

The unpaired nucleotides produced by the action of restriction enzymes are referred to as _____.

sticky ends

9

What enzyme forms covalent bonds between restriction fragments?

DNA ligase

10

In order to insert a human gene into a plasmid, both must _____

be cut by the same restriction enzyme