Microbio Labelling Flashcards


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created 6 years ago by Delanie_Rodriquez
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updated 6 years ago by Delanie_Rodriquez
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Serratia Marcescens

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Micrococcus luteus

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Ameba proteus

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Plasmodium falciparum

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Staphylococcus epidermidis

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Bacillus megaterium

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E Coli, Pseudomonas, and Myobacterium

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Trypanosoma cruzi

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Trichomonas Vaginalis

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Paramecium

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Bacillus megaterium

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Steps of Gram Stain

1. Crystal Violet (Primary) 1 min

2. Iodine (Mordant) 1 min

3. Alcohol (Decolorizer) 5-10 secs

4. Safranin (Counterstain) 1 min

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Acid Fast Stain

1. Carbolfuchsin (Primary) USE Bibulous Paper!

2. Heat (Mordant) 5-10 mins

3. Acid Alcohol (Decolorizer) 5-10 secs

4. Methylene Blue (Counterstain) 1 min

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Endospore Stain

1. Malachite Green (Primary) USE Bibulous Paper!

2. Heat (Mordant) 5-7 mins

3. Rinse with Water (Decolorizer)

4. Safranin (Counterstain) 1 min

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Gram Stain Positive Color and Why

Purple, Thick Peptidoglycan

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Gram Stain Negative Color and Why

Red, Thin Peptidoglycan

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Acid Fast Stain Positive Color and Why

Red, Contains Mycolic Cell Wall

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Acid Fast Stain Negative Color and Why

Blue, Lacks Mycolic Cell Wall

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Reason for Endospore Stain

To distinguish between metabolically active vegetative cells and dormant, resistant spores