Microbiology lab exam

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Microbiology
Chapters 1-6
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1

parts of the microscope

card image
2

cocci

round shape

3

diplococci

cocci that remain in pairs after dividing

4

streptococci

divides and remain attached in chainlike

5

tetrads

two planes and remain in groups of four

6

staphylococci

multiple planes. grapelike

7

bacilli

rodlike

8

single bacilli

single rods

9

diplobacilli

pairs after division

10

steptobacilli

chains

11

coccobacillus

oval like cocci

12

vibrio

curved rods. like a comma

13

spirillum

helical shape

14

spirochete

helical and flexible

15

spore staining

prepare slide

add culture

air dry (10min)

heat dry (5X)

put on noiling water

flood slide with malachite green

remove after 10mins and rinse with DI water

add safranin

wait 1min

perform microscopy

16

E.coli

gram negative

non acid fast

non sporulating

acid slant and acid butt

17

S. aureus

gram positive

non acid fast

non sporulating

18

bacillus

gram positive

acid fast

sporulating

19

M. smeg

acid fast

20

what test do you perform when a microbe is found to be catalase positive

coagulase test

DNAase

MSA

21

What happens to a medium when H2O2 is added to it

it bubbles which shows that it's catalase positive

22

which organisms came out to be catalase positive

S. aureus and S. epidermis

23

which organisms came out to be catalase negative

S.pyogenes

S. salvarius

S. fecalis

24

Which organism was MSA positive and what color did it turn

S. aureus; yellow

25

which organism was MSA negative and what color did it turn

S. epidermis; red

26

which organism had a clear halo after the DNAase test

S. aureus

27

which organism had no halo after the DNAase test

S. epidermis

28

Which organism clotted for the coagulase test

S. aureus

29

which organism didn't clot for the coagulase test

S. epidermis

30

Which organism turned black for the bile esculin test

S. fecalis

31

which organism turned green for the bile esculin test

S. pyogenes

S. salvaris

32

which organism turned yellow for the phenol red test

S. fecalis

33

which organism turned red for the phenol red test

s. pyogenes

S. salvarius

34

For the blood hemolysis test, which one was gamma

S. fecalis

35

which blood hemolysis came out to be beta

S. pyogenes

36

which blood hemolysis came out to be alpha

S. salvarius

37

what color does the organism turn when it's lactose fermenter

pink

38

what color does the organism turn when it's a non-lactose fermenter

colorless/white

39

what dye is first used in a gram staining

crystal violet followed by acetone decolorizer, then safranin

40

what dye is used in spore staining

malachite green

41

what was added to the TSI tubes

kovac's reagant

42

P. aer

pinkish

alkaline slant

alkaline butt

non lactose

43

P. vul

yellow slant

yellow butt

gas inside

black ppt

44

C. fir

black ppt

creates gas

yellow slant

45

K. pne

lactose fermenter

yellow everywhere

46

S. ent

black ppt only in acid

it turns the media yellow first

47

mordent for gram staining technique

iodine and moist heat

48

what dye is used as a counter stain

methylene blue

49

decolorizer for acid fast stain?

acid alcohol

50

decolorizer in gram staining

acetone alcohol