Microbiology an Introduction: Selective/Differential Media Flashcards


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1

Which types of media in this lab are selective for Gram+ organisms ?

Mannitol Salt Agar

2

on what basis are the Gram + selective media also differential?

for mannitol fermentation

3

Which types of media used in this lab are selective for Gram - organisms?

MacConkey agar and Eosin Methylene Blue

4

on what basis for the Gram- selective media also differential?

for lactose fermentation

5

what color is mannitol salt agar before it is inoculated

red

6

how can the appearance of MSA plate change with a positive differential result

color change to yellow

7

What color is MacConkey agar before it is inoculated?

Raspberry

8

How is the appearance of MAC plate change with a positive differential result?

pink with precipitate

9

what color is eosin methylene blue agar before it is inoculated?

burgundy purple

10

how is the appearance of EMB plate change with a positive differential result ?

shiny metallic green

11

What is the definition of a selective medium?

grow only certain types of organisms and actively preventing some types from growing

12

what is the definition of a differential medium?

grow all types of microbes but it causes some organisms to look different

13

Which type medium used in lab so far this semester is neither selective nor differential?

Tryptic Soy Agar

14

what is the purpose of differential media ?

for identification

15

what is the purpose of selective media ?

for identification

16

grow all types of microorganisms but it causes some organisms to look different

differential media

17

grow only certain types of organisms actively preventing some types from growing

selective media

18

what is enrichment media

it is designed to promote the growth of a rare organism

19

designed to promote the growth of rare organisms

enrichment media

20

which bacteria would it be if the eosin methylene blue plate is shiny metallic green?

Escherichia coli

21
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Escherichia coli

22

which bacteria would it be if in MacConkey agar is pink with precipitate ?

Escherichia coli

23
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Escherichia coli

24
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Enterobacter aerogenes

25

which bacteria would it be in eosin methylene blue if it were pink and moist ?

Enterobacter aerogenes

26

what bacteria would it be in MacConkey of it were pink without precipitate ?

Enterobacter aerogenes

27
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Enterobacter aerogenes

28
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Proteus vulgaris

29

what bacteria would it be on a MacConkey agar and it were no new color on plate and is brownish

Proteus vulgaris

30
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Proteus vulgaris

31

What is the bacteria eosin methylene blue if there is no color change?

Proteus vulgaris

32

strong lactose fermenter

Escherichia coli

33

medium lactose fermenter

Enterobacter aerogenes

34

does not ferment lactose

Proteus vulgaris

35
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Staphylococcus aureus

36

which bacteria would be on a mannitol salt agar if it were yellow

Staphylococcus aureus

37
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Staphylococcus epidermidis

38

what bacteria would it be if mannitol salt agar remains red, and maybe more pink?

Staphylococcus epidermidis

39

weak mannitol fermenter

Staphylococcus epidermidis

40

strong mannitol fermenter

Staphylococcus aureus

41

EMB selective

against Gram +, for Gram -

42

MAC selective

against Gram +, for Gram -

43

EMB differential

lactose fermentation

44

MAC differential

lactose fermentation

45

MSA selective

against Gram -, for Gram +

46

MSA differential

mannitol fermentation