Microbiology - chapter 9

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Microbiology
Chapter 9
exam question chapter 9
updated 4 years ago by marciacaroline
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1

The reaction catalyzed by reverse transcriptase is

mRNA - cDNA

2

Advantage of using E coli to make a human gene product

its genes are well known

3

Which of the following is an agricultural product made by DNA techniques?

a) frost retardant

b) Bacillus thuringiensis insecticide

c) nitrogen fixation

d) glyphosate resistant crops

4

if you have inserted a gen in the Ti plasmid, the next step is

inserting the Ti plasmid into Agrobacterium

5

Biotechnology involves the

a) use of microorganisms t make desired products, the use of animal cells t make vaccines, and the development of disease-resistant crop plants

6

Which of the following is a desired characteristics of DNA vectors used in gene cloning procedures

a) self-replication

b) has a selectable marker

c) circular form of DNA

d) replicate in several species

7

An advantage of synthetic DNA is the ability to

insert desired restriction sites into the DNA sequence

8

An advantage of cDNA is that it

lacks introns

9

Genomic libraries contain

gene introns

10

Pieces of DNA stored in yeast cell are called a

library

11

A population of cells carrying a desired plasmid is called a

clone

12

Self-replicating DNA used to transmit a gene from one organism to another is a

vector

13

The Human Genome Project was focused on

determine the nucleotide sequence of the entire human genome

14

The steps in the PCR procedure

1. Incubate at 94 C to denature DNA strands

2. Incubate at 60 C for primer hybridization

3. Incubate at 72 C for DNA synthesis

15

Which of the following techniques are used to introduce recombinant DNA into plants?

a) gene guns

b) protoplast fusion

c) Ti plasmids and Agrobacterium

d) electroporation

16

PCR can be used to identify an unknown bacterium because

the RNA primer is specific

17

The random shotgun method is used in

genome sequencing

18

Restriction enzymes are

Bacterial enzymes that destroy phage DNA

19

A vector is a self-replicating segment of DNA such as

plasmid or viral genome

20

A shuttle vector is a plasmid that is used to move pieces of DNA among organisms such as bacterial, fungal and plant cells

True

21

The Ti plasmid isolated from Agrobacterium can be used to insert DNA into any type of plant

False

22

The Bt toxin derived from Bacillus thuringiensis has been introduced into some crop plants to make them resistant to insect destruction

True