chapter 16 q & a

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1

The leading and the lagging strands differ in that

the leading strand is synthesized in the same direction as the movement of the replication fork, and the lagging strand is synthesized in the opposite direction.

2

A new DNA strand elongates only in the 5' to 3' direction because

DNA polymerase can only add nucleotides to the free 3' end.

3

What is the function of topoisomerase?

relieving strain in the DNA ahead of the replication fork

4

What is the role of DNA ligase in the elongation of the lagging strand during DNA replication?

It joins Okazaki fragments together.

5

Which of the following help(s) to hold the DNA strands apart while they are being replicated?

single-strand binding proteins

6

Which of the following would you expect of a eukaryote lacking telomerase?

a reduction in chromosome length in gametes

7

Which of the enzymes removes the RNA nucleotides from the primer and adds equivalent DNA nucleotides to the 3' end of Okazaki fragments?

IV DNA polymerase I

8

Which of the enzymes covalently connects segments of DNA?

I helicase

9

Which of the following sets of materials are required by both eukaryotes and prokaryotes for replication?

double-stranded DNA, four kinds of dNTPs, primers, origins

10

In a linear eukaryotic chromatin sample, which of the following strands is looped into domains by scaffolding?

the 30-nm chromatin fiber

11

Which of the following statements describes the eukaryotic chromosome?

It consists of a single linear molecule of double-stranded DNA plus proteins.

12

Which of the following statements describes chromatin?***

Heterochromatin is highly condensed, whereas euchromatin is less compact.