Eosin Methylene Blue Agar
- It contains the dyes eosin and methylene blue, which inhibit the growth of gram-positive bacteria and therefore select for gram-negative bacteria.
- Strong acid production by E. coli precipitates
What kind of streak plate is this?
eosin methylene blue
What kind of streak plate is this?
Casein Agar
Nutrient agar plate?
Right side
Citrate Test
A) (-)
B) (+)
Starch Hydrolysis
Left:(-)
Right: (+)
Casein Hydrolysis
Top: (-)
Bottom: (+)
Gelatin Hydrolysis
A) (+)
B) (-)
Catalase test
A) (-)
B) (+)
What test is this?
Lactose
What test is this?
Glucose
What test has a red top?
INC
Acid Fast Stain Procedure (pg.92)
1. Carbolfuchsin-->Heat fix (5 min)
2. Acid Alcohol (1 min)
3. Methylene Blue (1 min)
Gram Stain Procedure (pg.87)
1. Crystal violet (1 minute)
2. Iodine (1 minute)
3.
Alcohol (1 minute)
4. Safranin (1 minute)
Endospore Stain Procedure (pg.99)
1. Malachite Green over steam (10 min)
2. Water
3.
Safranin (30s)
Nitrate Reduction Test
I N C