Exercise 4-2 Micro Lab: Columbia CNA with 5% Sheep Blood Agar
Columbia CNA with 5% sheep blood
is a differential, undefined, selective media that allows growth of gram positive organisms and stops or inhibits growth of most gram negative organisms
Which 3 bacteria does the Columbia CNA help grow?
staphylococci, streptococci, enteroccocci
What 6 carbon energy sources are in the Columbia CNA?
2. digest of animal tissue
3. beef extract
4. yeast extract
5. corn starch
6. sheep blood
In Columbia CNA, sheep blood provides the _________ and yeast extract provides the ________
What attacks gram negative bacteria in the Columbia CNA and how?
antibiotics colistin and nalidixic acid (CNA) interfere with DNA replication and affect membrane integrity
Which three bacteria are CNA antibiotics effective against?
How does sheep's blood help with the differentiation of gram positive organisms?
based on hemolytic reaction