Summer Immuno Lecture 7 Flashcards

A naive lymphocyte first recognizes its specific antigen and begins adaptive immune activation. Where does this recognition phase occur?
A. Primary lymphoid organs
B. Secondary lymphoid organs
C. Peripheral infected tissue
D. Bone marrow sinusoids

What are three secondary lymphoid organs?

____ ____, ____, ____

Which organs are classified as primary lymphoid organs?

____ ____

____

A patient has impaired lymphoid follicle formation. Which immune structures would most directly share this abnormality?
A. Primary lymphoid organs
B. All lymphatic vessels
C. Secondary lymphoid organs
D. All inflamed tissues

The common anatomical structure shared by secondary lymphoid organs is which of the following?

_____ _____

Lymphoid follicles begin as loose networks of which cell type?

_____ _____ _____

Early lymphoid follicles form in regions especially rich in which lymphocyte population?

Follicular dendritic cells are already present by which developmental period?

Which cell type displays antigen primarily to B cells?

____ ____ ____

Which cell type presents antigen primarily to T cells?

____ ____ ____

delete

Follicular dendritic cells can also bind immune complexes using receptors for which antibody region?

____ region

A follicular dendritic cell attracts and activates B cells, leading to intense B-cell proliferation. Over time, this active follicle becomes what?

______ ______

Before adaptive immunity responds effectively to infection, which system must first detect danger?

______ system

B cells proliferating in germinal centers become fragile. Which cells rescue them from apoptosis?

Which secondary lymphoid organ lacks high endothelial venules?

High endothelial venules serve primarily as a doorway for which cells?
A. Neutrophils and eosinophils
B. B and T cells
C. Monocytes and basophils
D. Plasma cells and platelets

High endothelial venules allow lymphocytes to enter secondary lymphoid organs from which compartment?
A. Blood
B. Bone marrow
C. Germinal centers
D. Efferent lymph

High endothelial venules are regions within which vessel type?
A. Arteriole
B. Capillary
C. Venule
D. Lymphatic duct

In a lymph node, high endothelial venules are located in which region?

In a lymph node, B cells mainly accumulate in which area?

In a lymph node, T cells mainly accumulate in which area?

Conventional dendritic cells mainly accumulate in which lymph-node region?

Follicular dendritic cells are mainly located in which lymph-node region?

Lymph-node sinus walls are carpeted with which immune cells?

Movement of immune cells within secondary lymphoid organs is coordinated by which signal type?
A. Chemokines
B. Antibodies
C. Perforins
D. Integrins only

Chemokines are best described as which type of cytokine?
A. Apoptotic cytokines
B. Chemoattractive cytokines
C. Cytotoxic cytokines
D. Complement-fixing cytokines

Follicular dendritic cells produce which chemokine to attract naive B cells?
A. CCR7
B. ICOSL
C. CXCR5
D. CXCL13

After a B cell finds its cognate antigen, what happens to its CXCL13 receptor expression?
A. It increases sharply
B. It remains unchanged
C. It downregulates
D. It switches to CD28

After recognizing cognate antigen, an activated B cell upregulates which chemokine receptor?
A. CXCL13
B. CCR7
C. ICOSL
D. B7

CCR7 expression helps activated B cells migrate toward which region?
A. Medullary sinus
B. Thymic cortex
C. Blood-red pulp border
D. Cortex-paracortex border

The cortex-paracortex border is important because it allows meeting between which cells?
A. B cells and T cells
B. NK cells and neutrophils
C. Macrophages and eosinophils
D. Plasma cells and platelets

Activated helper T cells downregulate signals keeping them in which region?

Activated helper T cells upregulate which receptor to meet activated B cells?
A. CCR7
B. CXCL13
C. CXCR5
D. ICOSL

CXCR5 expression helps helper T cells congregate with activated B cells at which site?
A. Medullary cord
B. Follicle border
C. Splenic red pulp
D. Efferent lymphatic

B cells require help from activated helper T cells. Activated helper T cells also require help from which cells?
A. Activated B cells
B. Resting macrophages
C. Naive neutrophils
D. Medullary thymocytes

The reciprocal help between activated B cells and activated Th cells occurs through what mechanism?
A. Cell-cell contact
B. Antibody secretion only
C. Complement lysis
D. Cytokine diffusion only

Which B-cell surface protein binds CD28 on helper T cells?
A. CCR7
B. ICOSL
C. B7
D. CXCL13

Which B-cell surface protein binds ICOS on helper T cells?
A. B7
B. ICOSL
C. CCR7
D. CXCR5

Which helper T-cell protein binds B7 on the activated B-cell surface?
A. ICOS
B. CD28
C. CCR7
D. CXCR5

Which helper T-cell protein binds ICOSL on the activated B-cell surface?
A. CD28
B. CXCL13
C. CCR7
D. ICOS

A patient has impaired complement deposition on antigen. Which FDC function would be most directly reduced?
A. Binding opsonized antigen
B. Producing IL-2
C. Presenting peptide to T cells
D. Activating thymic selection

Which statement best captures secondary lymphoid organ function?

They support _____ _____

At the follicle border, an activated Th cell directly helps an activated B cell through which ligand-receptor pair?
A. ICOSL–ICOS
B. B7–CD28
C. CD40L–CD40
D. CXCL13–CXCR5

During the B-cell/Th-cell “dance,” which molecule is provided by the helper T cell?

During the B-cell/Th-cell “dance,” which molecule is found on the B-cell surface and binds CD40L?

Activated B cells provide which material to help helper T cells mature?
A. IL-17, IL-21, IL-23
B. Antigen, B7, ICOSL
C. CD40L, IL-2, FasL
D. Perforin, granzyme, IFN-gamma

A helper T cell becomes fully mature at the follicular border after contact with an activated B cell. What is it now called?
A. Th0 cell
B. Treg cell
C. Tfh cell
D. CTL cell

Follicular helper T cells are licensed to rescue which fragile cell population?
A. Germinal-center B cells
B. Naive T cells
C. Marginal-zone macrophages
D. Follicular dendritic cells

A germinal-center B cell receives Tfh help and avoids apoptosis. Which later processes can Tfh cells support?

____ ____ and ____

Somatic hypermutation takes place in the ____ ____ of the germinal center

During affinity maturation, B cells with higher-affinity receptors gain an advantage by doing what?
A. Secreting more IL-12
B. Plucking more antigen
C. Blocking CD40 signaling
D. Losing class II MHC

Higher-affinity B cells present more antigen to Tfh cells using which molecule?

In germinal centers, greater antigen presentation by high-affinity B cells causes what outcome?

Somatic hypermutation selects higher-affinity B cells because these cells receive more help from which cells?

A killer T cell recognizes cognate antigen presented by dendritic cells in a lymph node. Where does activation occur?

A lymph node draining an infected wound becomes enlarged. Which process contributes most directly?

_____ proliferation

In infected draining lymph nodes, medullary sinuses may become clogged by which cells?

_____

Peyer patches are examples of which lymphoid tissue?
A. Thymic tissue
B. Red pulp
C. Bone marrow
D. MALT

Unlike lymph nodes, Peyer patches lack which structure?
A. Outgoing lymphatics
B. Incoming lymphatics
C. Germinal centers
D. B-cell follicles

Peyer patches are topped by which specialized epithelial cells?
A. Goblet cells
B. Paneth cells
C. Enteroendocrine cells
D. M cells

M cells over Peyer patches are not coated with which structures?

____ and ____

M cells sample material from which location?

____ ____

M cells transport sampled intestinal material into which site?

____ ____

The spleen primarily functions as what type of filter?
A. Blood filter
B. Lymph filter
C. Mucus filter
D. Airway filter

Approximately what fraction of cardiac output enters the spleen?
A. 1%
B. 20%
C. 5%
D. 50%

The spleen screens circulating blood approximately every how long?
A. 5 minutes
B. 30 minutes
C. 6 hours
D. 1 day

Compared with lymph nodes and Peyer patches, the spleen is less selective because everything enters from where?
A. Blood
B. Lymph
C. Mucus
D. Thymus

Which organs are selective about where B/T cells and antigens enter?

_____ _____ and _____ _____

In the spleen, T cells are temporarily retained in which region?

_____

In the spleen, B cells are found between which structures?

_____ and _____ _____

The periarteriolar lymphocyte sheath mainly retains which cells?
A. T cells
B. B cells
C. Plasma cells
D. Macrophages

The spleen lacks lymphatics to transport dendritic cells from tissues. How does it compensate?
A. Uses thymic epithelial cells
B. Uses incoming M cells
C. Uses circulating neutrophils
D. Uses resident dendritic cells

In the spleen, resident dendritic cells take up antigens from invaders located where?

Which organ is a main site where B cells can activate without Th-cell assistance?

B cells that can activate without Th-cell help in the spleen are called what?
A. Germinal-center B cells
B. Naive follicular B cells
C. Plasma memory cells
D. Marginal-zone B cells

Marginal-zone B cells are stationed where they contact blood entering the spleen. Where is this?
A. Marginal sinuses
B. Red pulp cords
C. Splenic capsule
D. Central arteriole wall

Secondary lymphoid organs are strategically positioned to intercept which threat?
A. Self-reactive thymocytes
B. Aging erythrocytes only
C. Invaders entering the body
D. Bone marrow precursors

Secondary lymphoid organs provide environments that mobilize weapons appropriate to what?
A. Thymocyte selection stage
B. Likely invading organisms
C. Red-cell deformability
D. Platelet activation status

The most important function of secondary lymphoid organs is bringing together which cells?
A. Platelets and erythrocytes
B. Mast cells and basophils
C. Fibroblasts and osteoblasts
D. Lymphocytes and APCs

Bringing lymphocytes and APCs together increases the probability of activating which immune system?
A. Adaptive immune system
B. Complement system
C. Coagulation system
D. Innate barrier system

The process of B and T cells moving to specific locations is called what?
A. Chemotactic drift
B. Lymphocyte trafficking
C. Germinal rescue
D. Antigen plucking

Virgin and experienced lymphocytes differ mainly in which feature?
A. Nuclear membrane number
B. Immunoglobulin shape
C. Traffic patterns
D. Ribosomal density

Virgin T cells use which molecule to enter lymph nodes through HEVs?

L-selectin on virgin T cells binds which ligand on lymph-node HEVs?
A. MadCAM-1
B. ICOS
C. CD40
D. GlyCAM-1

Virgin T cells use which integrin to enter Peyer patches and intestinal lymph nodes?
A. CD28
B. α4β7
C. CXCL13
D. CD40L

α4β7 on virgin T cells binds which ligand on intestinal HEVs?
A. GlyCAM-1
B. Fas receptor
C. MadCAM-1
D. ICOSL

Virgin T cells circulate through blood and lymph, spending about how long per loop?

If a virgin T cell does not find cognate antigen, it dies after approximately how long?
A. 1 day
B. 30 minutes
C. 1 year
D. 6 weeks

A virgin T cell failing to find cognate antigen eventually dies by what mechanism?

Experienced T cells that have found cognate antigen have their travel passports become what?
A. Expanded
B. Deleted
C. Randomized
D. Restricted

Experienced T cells express adhesion molecules based on what location?
A. Where they activated
B. Where they die
C. Where they circulate randomly
D. Where antibodies bind

A T cell activated in a Peyer patch will increase which adhesion molecule?
A. GlyCAM-1
B. CD40
C. α4β7
D. B7

Increased α4β7 on Peyer-patch-activated T cells helps them do what?
A. Return to mucosal sites
B. Enter thymic cortex
C. Bind splenic red pulp
D. Block dendritic migration

Experienced T cells also carry “combat passports” that help them enter what sites?
A. Primary lymphoid organs
B. Sites of infection
C. Bone marrow niches
D. Thymic medulla

Experienced mucosa-activated T cells express which integrin?
A. α4β7
B. MadCAM-1
C. L-selectin
D. αEβ7

αEβ7 helps experienced mucosal T cells enter which tissue environment?
A. Inflamed mucosa
B. Splenic capsule
C. Thymic cortex
D. Bone marrow

A defect in CD40L expression on activated Th cells would most directly impair which interaction?
A. B-cell CD40 activation
B. HEV entry into nodes
C. Antigen plucking by FDCs
D. M-cell sampling

A B cell fails to provide B7 and ICOSL to a helper T cell. Which process is most directly impaired?
A. CTL granzyme release
B. Helper T-cell maturation
C. M-cell antigen transport
D. Splenic blood screening

A high-affinity B cell outcompetes low-affinity B cells in a germinal center. Which mechanism best explains this?
A. Less antigen presentation
B. Better antigen plucking
C. Loss of class II MHC
D. Reduced Tfh contact

A patient’s draining lymph node is swollen after cellulitis. Which combined mechanism best explains the swelling?
A. Lymphocyte loss, neutrophil apoptosis
B. Platelet trapping, RBC hemolysis
C. Lymphocyte growth, macrophage clogging
D. Fibrosis, thymocyte expansion

A virgin T cell enters intestinal lymphoid tissue. Which receptor-ligand pair is most likely used?
A. CD40L–CD40
B. L-selectin–GlyCAM-1
C. α4β7–MadCAM-1
D. ICOS–ICOSL

A virgin T cell enters a nonintestinal lymph node through HEVs. Which pair is most relevant?
A. αEβ7–mucosal ligand
B. α4β7–MadCAM-1
C. ICOS–ICOSL
D. L-selectin–GlyCAM-1

Structures (in order) from arteriole to venulue as lymph passes a lymph node?

(Medullary Sinus, Cortex, Paracortex, Marginal Sinus)

The subcapuslar sinus of a lymph node contains what two cells?

delete

Where in the spleen does blood first enters and contains resident dendritic cells?

____ ____

____ bacteria can directly activate B-cells without T-cell help.

Peyer’s Patches specialize in turning out Th Cells that secrete ____ cytokine types and B-Cells that secrete ____.

If you are invaded by bacteria, the lymph node will produce _____ cytokine types and B-Cells that secrete _____.