Summer Immuno Lecture 2 Flashcards

Which organ normally produces most complement proteins?

A researcher measures complement proteins in serum. Which protein should be most abundant?

In the _____ complement pathway, C3 is continuously split into which products?

Which C3 fragment is highly reactive after spontaneous C3 breakdown?

C3b rapidly binds chemical groups on nearby bacterial surfaces. Which groups are targeted?
A. Amino or hydroxyl
B. Sulfhydryl or methyl
C. Phosphate or carboxyl
D. Aldehyde or ketone

If C3b fails to bind a target group quickly, it is neutralized. What is the approximate time window?
A. 6 seconds
B. 60 seconds
C. 6 microseconds
D. 60 microseconds

Free C3b that misses a bacterial surface is neutralized by which molecule?
A. Oxygen
B. Water
C. Glucose
D. Albumin

After C3b stabilizes on a bacterial surface, which complement protein binds next?
A. C5
B. C9
C. B
D. MASP

After factor B binds stabilized C3b, which protein clips factor B?
A. D
B. C9
C. C5
D. MBL

Cleavage of factor B on surface-bound C3b forms which complex?
A. C3bC5b
B. C5b678
C. C4b2a
D. C3bBb

C3bBb enzymatically cleaves additional C3 molecules. What type of enzyme is C3bBb?
A. Ligase
B. Convertase
C. Polymerase
D. Kinase

In the alternative pathway, C3bBb primarily cleaves which complement protein?

C3bBb generates more C3b, which can form more C3bBb. What does this create?
A. Positive feedback
B. Negative feedback
C. Competitive inhibition
D. Clonal deletion

C3bBb can bind another molecule to become a C5-cleaving complex. Which molecule is added?
A. C1q
B. C4b
C. MASP
D. C3b

The C3bBb-C3b complex cleaves which complement protein?

C5 cleavage produces which two fragments?
A. C5a and C5b
B. C3a and C3b
C. C4a and C4b
D. C6a and C6b

Which complement fragment initiates membrane attack complex assembly?
A. C3a
B. C4b
C. C5b
D. C5a

C5b combines with which proteins to form the MAC?

In MAC formation, which components form the membrane-anchoring stalk?

Which MAC component forms the membrane pore?
A. C9
B. C8
C. C5a
D. C3b

A gram-negative bacterium dies after complement forms a pore through its membrane. Which outcome occurred?
A. Antigen presentation
B. Thymic selection
C. Bacterial lysis
D. Class switching

Human cells avoid complement injury partly because blood enzymes inactivate which molecule?

Which cell-surface protein accelerates C3b inactivation?

_____ _____ _____

Which cell-surface protein accelerates C3bBb destruction?

____ ____ ____

CD59 is also known by which name?
A. Properdin
B. Protectin
C. Factor D
D. Factor B

CD59 protects host cells by preventing which event?
A. C3 cleavage
B. C5a release
C. Factor B binding
D. C9 attachment

The central recognition molecule in the lectin pathway is which protein?
A. MBL
B. MASP
C. C3bBb
D. CD59

Mannose-binding lectin is central to which activation pathway?
A. Classical
B. Alternative
C. Lectin
D. Terminal

In blood, mannose-binding lectin binds which associated protein?
A. Factor B
B. MASP
C. C9
D. C5b

In the lectin pathway, MBL attaches to which bacterial surface sugar?
A. Glucose
B. Galactose
C. Fructose
D. Mannose

In the lectin pathway, which component acts as the convertase?
A. MASP
B. MBL
C. C9
D. MCP

In the lectin pathway, MASP clips which complement protein?
A. C5
B. C9
C. C3
D. Factor B

The classical pathway depends on which immune molecule?
A. Mannose
B. C3bBb
C. B2-microglobulin
D. Antibody

Which pathway is antibody-independent?
A. Classical pathway
B. Alternative pathway
C. Fc receptor pathway
D. Plasma cell pathway

C3b can be clipped into an inactive opsonin. What is it called?
A. iC3b
B. C3a
C. C5b
D. C9

iC3b still helps immune defense mainly by promoting which process?

Which two complement fragments help form MACs?

Which complement fragments act as chemoattractants?
A. C3a and C5a
B. C3b and C5b
C. C6 and C7
D. C8 and C9

C3a and C5a recruit immune cells toward inflammation. What is this function called?

Which complement fragment is especially powerful as a chemoattractant?
A. C3b
B. C5a
C. C5b
D. C9

C5a is especially powerful at attracting which immune cells?

_______

C3a and C5a are also classified as what?
A. Anaphylatoxins
B. Immunoglobulins
C. Opsonins
D. Convertases

C3a and C5a can contribute to which systemic reaction?
A. Hemolytic anemia
B. Neutropenic fever
C. Anaphylactic shock
D. Thymic aplasia

Which cells are considered professional phagocytes?
A. B cells and T cells
B. Eosinophils and basophils
C. Plasma cells and mast cells
D. Macrophages and neutrophils

Which professional phagocyte acts as a sentinel cell in tissues?
A. Neutrophil
B. Macrophage
C. B cell
D. Platelet

Where are macrophage sentinel cells typically positioned?
A. Below exposed tissue surfaces
B. Inside erythrocyte cytoplasm
C. Within antibody constant regions
D. Attached to C9 pores

Macrophage sentinels are especially found near tissues exposed to what?
A. Bone marrow
B. Thymic cortex
C. External environment
D. Red pulp

A C3 molecule spontaneously breaks apart near a bacterial membrane. Why must this occur very close to the target?
A. C3a binds only nuclei
B. C3b is rapidly neutralized
C. C9 blocks bacterial binding
D. MASP destroys C3b

A tissue macrophage is primed by inflammatory cytokines and begins presenting antigen to helper T cells. Which molecule is upregulated?
A. Class I MHCs
B. IgE receptors
C. Class II MHCs
D. C9 channels

A resting macrophage becomes an antigen-presenting cell after activation. What change best explains this?
A. Increased class II MHC
B. Decreased lysosome number
C. Loss of cytokine secretion
D. Reduced antigen display

A macrophage is exposed to IFN-gamma during infection. What is the expected functional result?
A. Antibody secretion
B. Neutrophil apoptosis
C. Hemoglobin production
D. Macrophage priming

Which cytokine is best known for priming resting macrophages?

IFN-gamma is produced mainly by which cells?
A. Plasma cells and eosinophils
B. Mast cells and basophils
C. Neutrophils and monocytes
D. Helper T cells and NK cells

A macrophage receives direct stimulation from bacterial lipopolysaccharide. What state can it enter? ______

Lipopolysaccharide is classically associated with which bacteria?

A hyperactivated macrophage releases a cytokine that can kill tumor cells and virus-infected cells. Which cytokine is this?

TNF produced by hyperactivated macrophages can directly help kill which target?
A. Virus-infected cells
B. Resting macrophages
C. Red blood cells
D. Memory B cells

Besides killing abnormal cells, TNF also helps perform which function?
A. Produce hemoglobin
B. Form neutrophil NETs
C. Block all cytokines
D. Activate immune cells

A hyperactivated macrophage increases what organelle?

_____

A macrophage can also increase production of _____ _____ during hyperactivation.

A macrophage encounters a parasite too large to ingest. Which hyperactivated ability helps attack it?
A. Antibody secretion
B. BCR expression
C. Lysosomal dumping
D. Hemoglobin release

A neutrophil leaves bone marrow. On average, when will it die?
A. About 5 days
B. About 3 weeks
C. About 1 month
D. About 60 microseconds

Compared with macrophages, neutrophils are generally not which type of cell?
A. Phagocytes
B. Innate immune cells
C. Antigen-presenting cells
D. Short-lived cells

Neutrophils uniquely ____ tissues with destructive enzymes during infection.

A dying neutrophil releases web-like structures that trap bacteria and fungi. What are these called?
A. MHC complexes
B. Selectin ligands
C. NETs
D. Antibodies

Neutrophil extracellular traps (NETs) can trap or kill which organisms?
A. Bacteria only
B. Bacteria, viruses, fungi, parasites
C. Viruses only
D. Parasites only

Before infection, endothelial cells display which adhesion molecule?

Neutrophils normally carry which surface molecule for rolling?
A. Selectin ligand
B. ICAM
C. TNF
D. MBL

During infection, nearby macrophages release which two cytokines to activate endothelium?

IL-1 and TNF cause endothelial cells to produce which molecule?

Selectin on endothelium binds which neutrophil molecule?
A. ICAM
B. Integrin
C. MHC II
D. Selectin ligand

Selectin binding to neutrophil selectin ligand causes what?
A. Neutrophil lysis
B. Antibody secretion
C. Neutrophil rolling
D. Class switching

While rolling, a neutrophil samples nearby tissue for which signals?
A. Inflammatory signals
B. Antibody chains
C. Hemoglobin fragments
D. Thymic hormones

Which inflammatory signal can a rolling neutrophil detect?

____

Which bacterial signal can help activate a rolling neutrophil?
_____

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Neutrophil integrin binds which endothelial molecule?
A. Selectin ligand
B. MBL
C. ICAM
D. C9

Integrin binding to ICAM causes which neutrophil behavior?
A. Faster rolling
B. Antibody release
C. Thymic exit
D. Full stopping

A neutrophil firmly adheres to endothelium after integrin-ICAM binding. What comes next?
A. MHC II loss
B. Exit to tissue
C. IgG secretion
D. B-cell activation

Which chemoattractant encourages stopped neutrophils to enter tissue?

Bacterial protein fragments containing formyl methionine are also called what?
A. C5a peptides
B. LPS peptides
C. f-met peptides
D. MHC peptides

f-met peptides help guide _____ toward the _____ site.

A macrophage detects gram-negative LPS before major adaptive activation. Which receptor group enables detection?
A. B-cell receptors
B. Pattern-recognition receptors
C. Antibody constant regions
D. T-cell receptors only

Pattern-recognition receptors are used by immune cells to recognize what?
A. Invasion patterns
B. Hemoglobin variants
C. Antibody classes
D. Thymic hormones

Pattern-recognition receptors detect pathogen-associated molecular patterns, also called what?
A. DAMPs
B. NETs
C. PAMPs
D. MACs

Pattern-recognition receptors detect damage-associated molecular patterns, also called what?
A. NETs
B. PAMPs
C. MHCs
D. DAMPs

LPS from gram-negative bacteria is an example of what?
A. DAMP
B. PAMP
C. NET
D. MAC

Intracellular molecules released after cell death are examples of what?
A. PAMPs
B. Antibodies
C. DAMPs
D. Selectins

Approximately how many receptor types are included among Pattern-recognition receptors?
A. Two
B. Five
C. Ten
D. Over twenty

Which PRR family is described as most studied?
A. Integrins
B. Toll-like receptors
C. Selectins
D. Immunoglobulins

How many Toll-like receptors are described in this material?
A. Ten
B. Five
C. Three
D. Twenty

Which Toll-like receptor detects lipopolysaccharide?

TLR4 is anchored in which location?

____ ____

TLR4 points in which direction?
A. Inward
B. Outward
C. Nuclear
D. Cytosolic only

Which TLR detects viral single-stranded RNA?

TLR7 detects ssRNA from viruses such as which examples?
A. Influenza and HIV-1
B. E. coli and Salmonella
C. HSV and bacteria
D. Staph and Strep

Which TLR detects double-stranded DNA?

TLR9 detects dsDNA from which sources?
A. Influenza and HIV-1
B. Bacteria and herpes simplex
C. Fungi and parasites
D. E. coli LPS only

TLR7 and TLR9 are located on which structure?

_____

TLR7 and TLR9 point in which direction?
A. Inward
B. Outward
C. Extracellular
D. Nuclear

Which molecule lets endothelium slow neutrophils during inflammation?
A. Integrin
B. Selectin
C. IFN-gamma
D. f-met

A neutrophil exits blood by squeezing between endothelial cells. What drives this movement?
A. Hemoglobin binding
B. Antibody neutralization
C. Chemoattractant gradients
D. Class switching

There are two important characteristics of ____:

1) They recognize ____ characteristics of classes of invaders.
2) They recognize specific structural features that are so important to pathogens that they cannot be easily altered or mutated, as the pathogen would not likely ____ the change.

The innate immune system’s primary defense against viruses is which system?

____ system

A virally infected cell’s PRRs detect viral material. Which warning proteins are produced?

IFN-alpha and IFN-beta mainly interfere with which process?
A. Bacterial chemotaxis
B. Viral reproduction
C. Antibody class switching
D. Neutrophil rolling

IFN-alpha and IFN-beta are classified as which interferon type?
A. Type II
B. Type III
C. Type IV
D. Type I

IFN-gamma is classified as which interferon type?
A. Type I
B. Type II
C. Type III
D. Type IV

Type I interferons bind virus-infected cells and induce which response?
A. Antiviral protein production
B. Factor D cleavage
C. Antibody secretion
D. NET formation

An uninfected cell binds interferon but has no viral attack. What happens initially?
A. Immediate apoptosis
B. Antibody secretion
C. Business as usual
D. Class II loss

An interferon-warned cell later becomes infected by virus. What is the expected outcome?
A. Proliferation
B. Apoptosis
C. Opsonization
D. Phagocytosis

Many cells can produce type I interferon, but which WBC produces the most?
A. Neutrophil
B. Plasma B cell
C. Helper T cell
D. Plasmacytoid dendritic cell

Plasmacytoid dendritic cells are especially important for producing which molecules?
A. IFN-alpha and IFN-beta
B. IgG and IgA
C. C5b and C9
D. IL-2 and histamine

Natural killer cells defend against infections partly by producing what?

Which cytokine can natural killer cells produce?

Besides cytokine production, NK cells defend by forcing target cells into what process?
A. Apoptosis
B. Opsonization
C. Neutralization
D. Class switching

NK cells can inject suicide enzymes into target cells using which protein?
A. Perforin
B. Selectin
C. ICAM
D. MASP

Perforin helps NK cells deliver which molecules?
A. Complement proteins
B. Suicide enzymes
C. Antibody chains
D. MHC molecules

NK cells can also trigger apoptosis through which surface ligand?
A. Fas ligand
B. Selectin ligand
C. IL-2 ligand
D. C3b ligand

Fas ligand on an NK cell binds which target-cell protein?
A. C3b
B. Fas
C. ICAM
D. MBL

Fas-Fas ligand interaction causes the target cell to do what?
A. Self-destruct
B. Present antibodies
C. Release hemoglobin
D. Produce C9

NK cells differ from T cells because NK cells lack what?
A. Cytokines
B. T-cell receptors
C. Inhibitory receptors
D. Activating receptors

Instead of T-cell receptors, NK cells use which receptor types?
A. Activating and inhibitory
B. Heavy and light
C. Alpha and beta
D. Soluble and secreted

NK activating receptors motivate the cell to do what?
A. Not kill
B. Kill
C. Secrete antibody
D. Mature thymically

NK inhibitory receptors motivate the cell to do what?
A. Not kill
B. Kill immediately
C. Produce antibodies
D. Form MACs

The NK “don’t kill” signal comes from recognition of which molecule?
A. Class II MHC
B. Class I MHC
C. IgG
D. C3bBb

The NK “kill” signal comes from recognition of unusual surface what?

_____ and _____

Unusual foreign carbohydrates or proteins suggest a cell is stressed by what?
A. Infection or cancer
B. Normal differentiation
C. Thymic maturation
D. Antibody secretion

A virus hijacks a host cell and stops class I MHC production. Which immune cell may miss it?
A. Neutrophil
B. Killer T cell
C. NK cell
D. Macrophage

Why would a class I MHC-deficient infected cell evade killer T cells?
A. No class I display
B. Excess IFN-gamma
C. Too much C3b
D. More Fas ligand

A class I MHC-deficient infected cell is placed near an NK cell. What happens?
A. NK ignores it completely
B. NK receives “don’t kill” signal
C. NK likely induces apoptosis
D. NK becomes a plasma cell

Why do NK cells kill cells with low class I MHC?
A. They detect antibodies
B. They lack inhibitory signal
C. They require BCR signaling
D. They bind class II MHC

In NK-macrophage cooperation, bacterial LPS first binds which cell?

____ cell

After LPS stimulation, NK cells produce which macrophage-priming cytokine?

IFN-gamma from NK cells does what to macrophages?
A. Primes macrophages
B. Destroys macrophages
C. Blocks TNF release
D. Prevents LPS binding

Primed macrophages can become hyperactivated by direct contact with what?
A. IgD
B. LPS
C. Hemoglobin
D. IL-2

A hyperactivated macrophage produces which cytokine in this feedback loop?

After producing TNF, the macrophage responds by secreting which cytokine?

IL-12 plus TNF influences NK cells to increase production of what?

In the NK-macrophage loop, TNF increases NK-cell expression of which receptor?
A. IL-2 receptors
B. T-cell receptors
C. B-cell receptors
D. Class II receptors

IL-2 acts as what for NK cells?

____ ____

IL-2 allows NK cells to do what?
A. Proliferate
B. Form antibodies
C. Express hemoglobin
D. Make C9 pores

In the NK-macrophage loop, which cytokine primes macrophages?
A. TNF
B. IFN-gamma
C. IL-2
D. IL-12

In the NK-macrophage loop, which cytokine helps induce IL-12 release?
A. TNF
B. IFN-alpha
C. IL-2
D. IgE

After IFN-gamma primes macrophages, what can LPS directly trigger?
A. Hyperactivation
B. Class I loss
C. Antibody switching
D. NET release

Which two cytokines together increase NK IFN-gamma output?
A. IL-12 and TNF
B. IL-2 and IFN-beta
C. IgG and IgA
D. C3a and C5a

Which cytokine causes NK cells to upregulate IL-2 receptors?
A. TNF
B. IFN-alpha
C. IFN-beta
D. IL-4

Activated macrophages can produce which complement protein?
A. C3
B. C9
C. C5a
D. CD59

Activated macrophages can produce C3 and which factors?
A. Factors B and D
B. Factors H and I
C. Factors C and E
D. Factors A and C

Complement proteins start being made during which fetal period?
A. First trimester
B. Second trimester
C. Third trimester
D. After birth

NK cells mature in the ____ ____ and found in blood, spleen or liver.

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