Light Microscopy Flashcards


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created 7 days ago by sophiaisstudying
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1

What is the Eyepiece lens used for?

It is looked through to see the image and also magnifies the image.

2

What is an Objective lens used for?

magnifying the image, there are typically three different objective lenses

3

What is the Stage used for?

It supports the slide

4

What is the Clip used for?

It holds the slide in place

5

What is the Handle used for?

To carry the microscope

6

What is the Lamp used for?

It shines a light up through the slide so the image can be seen more easily

7

What are the Focusing Knobs used for?

They bring the stage up and down to bring the image into focus.

8

Why do you need a thin slice of your specimen?

It needs to be able to let light through for you to be able to see it clearly

9

How do you secure the specimen in place?

With a pipette put either one drop of water or mountant in the middle of the clean slide

10

What is Mountant?

A clear, gloopy liquid

11

How should you place your specimen on the slide?

Using tweezers

12

In what situation will you need to use a stain?

If your specimen is completely transparent or clear, a drop of stain will be necessary to make it visible

13

Why do you press the cover slip with a needle?

To get rid of air bubbles

14

Whats the first thing you need to do when your ready to view?

Clip the slide with your specimen onto the stage.

15

What lens do you FIRST set?

The lowest-powered objective lens (the one that produces the lowest magnification)

16

Which knob do you use to move the stage up and down?

The coarse adjustment knob

17

Which knob do you use to adjust the focus and get a clear image?

Fine adjustment knob

18

What do you do if you need a higher magnification?

Switch to a higher-powered objective lens and refocus.