Microbiology Lecture Midterm Flashcards


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1

use of chemical agents to harm or kill microbes is called?

Chemotherapy

2

the first disease shown to be bacterial in origin

Anthrax

3

experiments conducted by John T. Needham using infusions…….which theory he supported?

Spontaneous Generation

4

following bacterial cell structures plays an important role in the creation of biofilms?

Flagella

5

Cholesterols are typically found in ________?

Eukaryotic cytoplasmic membrane

6

Lysosomes result from the endocytosis of food particles by eukaryotic cells. True or false

False

7

Carbolfuchsin is a dye used in….what kind of stains?

Both the acid fast and flagellar

8

The kingdoms included in the Linnaeus system of classification are?

Animal and plant

9

The function of a mordant in staining

~Fix the specimen to the slide

~ Intensifying stains in cell or tissue preparations

10

In Gram staining, ethanol-acetone is used...

Decolorizing agent

11

A single basic dye is used in simple stains. True or false

True

12

Anabolic reactions may be characterized as

Forming large molecules from smaller molecules

13

An organic compound gains an electron from another molecule. The organic compound…?

Has been reduced

14

The molecule that an enzyme acts upon is known as its..

Substrate

15

Which of the following statements concerning enzymes is FALSE…

a. They are usually, but not always, proteins.
b. They form a temporary intermediate compound with a substrate.
c. They can be denatured if the pH of their environment is too high or too low.
d. They can be used to catalyze a chemical reaction over and over again.
e. They always function best at 37°C.

E. They always function best at 37C

16

Following statements concerning glycolysis is TRUE?

A) It produces ATP by oxidative phosphorylation.
B) It occurs in the cell membranes of bacteria.
C) It both requires the input of ATP and produces ATP.
D) It is an alternative to fermentation.
E) It involves ribulose 5-phosphate as an intermediate

C) It both requires the input of ATP and produces ATP.

17

Many vitamins are important for the enzymes because ……?

Coenzymes

18

What is the final product in glycolysis…

two pyruvate (pyruvic acid) molecules

19

As the concentration of substrate increases, the activity of the enzyme decreases.

False

20

A fastidious organism might be grown on which of the following types of media

Enriched media

21

MacConkey agar plates represent? What kind of medium?

Both a differential and selective

22

A microorganism found living under conditions of high ________ is a barophile

Hydrostatic pressure

23

Photoheterotrophs use carbon dioxide as their sole carbon source.. True or False

False

24

Following statements is true of bacterial plasmids

A) They are found in the nucleoid.
B) They can replicate autonomously.
C) They carry genes for essential metabolic functions.
D) They are small circular DNA molecules.
E) They are small circular DNA molecules that can replicate autonomously.

E) They are small circular DNA molecules that can replicate autonomously.

25

Following is involved in translation

~mRna

~tRna

~rRna

26

Semiconservative DNA replication means that

Every double helix in the new generation of an organism consists of one complete “old” strand and one complete “new” strand wrapped around each other

27

Which are frameshift mutations….

Insertion mutations and deletion mutations

28

Frederick Griffith discovered…What ?

was the first to reveal the “transforming principle,” which led to the discovery that DNA acts as the carrier of genetic information.

29

The term "semiconservative replication" means that both strands of a DNA molecule are a mix of newly replicated and original template DNA... True or False

False

30

The phenotype of an organism reflects only part of its genotype….true or false

True

31

Use of microorganisms to produce useful products is known as

Biotechnology

32

Mutagens are useful in biotechnology research for

Producing organisms with altered phenotypes

33

What is the function of the high temperature step in PCR (polymerase chain reaction)?

Break hydrogen bonds for strand separation

34

Gene therapy.

A medical approach that treats or prevents disease by correcting the underlying genetic problem; Altering the genes inside your body's cells in an effort to treat or stop disease.

35

The microbes residing on human skin are part of the human microbiome... True or False

True

36

Endospores

~Produced by Gram-positive bacteria Bacillus and Clostridium

~Are stable resting stages that barely metabolize

~Can be difficult to destroy. Several species of bacteria produce toxins that cause disease.

37

Mycoplasmas are pleomorphic because

They have no cell walls

38

anaerobic Clostridium species are troublesome pathogens largely because of their capacity for

Endospore production

39

Mycobacterium includes species responsible for

Tuberculosis

40

The fusion of two gametes produces a…????

Zygote

41

Merozoites are a result of

Schizogony

42

Sexual reproduction in multicellular Ascomycota leads to the production of..What ?

Asci

43

Hyphae are associated with which of the following

Mold

44

Lichens are composed of a symbiosis of fungi and which of the following

Alga or cyanobacteria

45

Fertilized chicken eggs are used to culture some vaccine strains.

True

46

In what ways do viruses differ from other pathogens?

~Viruses lack cytoplasm and organelles.

~Viruses lack genetic material.

~Viruses are composed of both protein and lipid.

47

A(n) ________ is a virus that infects bacterial cells.

Bacteriophages

48

Host specificity of a virus is due to

Interactions between viral and cellular surface molecules

49

How are prions different from all other known infectious agents?

They lack nucleic acid

50

What is a virion?

~Complete virus particles and include a nucleic acid, a capsid, and in some cases an envelope

~an extracellular virus consisting of a protein coat (capsid) a nucleic acid core, either DNA or RNA, together known as a nucleocapsid called an envelope.