Microbiology: Post-Lab Quizzes (1-7) Flashcards

Those organisms that pose very little risk of disease for healthy students and they can still be capable of causing infection under certain circumstances are rated

Though we will not use these organisms during lab, these organisms can cause disease in healthy adults, pose a lethal risk, and do not respond to vaccines or antimicrobial therapies

Which container would you put a used coverslip in?

Which sections of the SDS contain general information about the chemical, identification hazards, compositions, safe handling practices, and emergency control measures

Which machine is used to heat a mixture and stir a mixture using electromagnetic forces?

Which piece of equipment measures the amount of material based on absorbance?

Which tool is used to move microorganisms between cultures and can be repeatedly sterilized?

Which machine agitates a mixture in a test tube to make a homogenized mixture?

Which piece of glassware is used to move liquid culture around an agar plate evenly?

Which pipet moves a fixed volume?

Which lenses are typically binocular?

Which objective lens is used for quickly scanning the microscope slides?

This type of microscope causes the light source to hit the specimen at a severely oblique angle

This is the bending of light as it passes from one medium to another

Which one of the following phylum is considered an algae?

Which of the following is not in the group Alveolata?

Which one of the following bacterial phyla is Gram positive?

Which phylum contains the plant pathogenic fungi known as smuts and rusts?

Which phylum is an animal microbe?

Which virus classification does not match the description?

Class VI- double stranded DNA with reverse transcriptase

Which type of stain does not stick to the cell, but dries around the cell boundary creating a silhouette?

Which type of stain uses both basic and acidic dyes?

Which type of stain uses two dyes- a primary dye and a counterstain?

This type of differential stain reacts to a waxy material called mycolic acid in the bacterium's cell wall

The method completed in lab using crystal violet and safranin

With Gram staining, Gram positive bacteria will stain as which color?

Which spore staining, vegetative cells will appear as which color?

With acid-fast staining method, non-acid-fast bacteria will stain which color?

The first step in smear preparation from a broth is?

Differential staining with an endospore stain _______ a heat-fixed smear

These media contain agar for thickening but do not form a firm substrate

These media contain substances that absorb oxygen or slow the penetration of oxygen

This type of media contains one or more agents that inhibit the growth of certain microbes but not another

This type of media has large molecules such as proteins, polysaccharides, and lipids that can vary greatly in exact composition

When agar media is placed in a test tube and is allowed to solidify vertically, it is called a

This media is a general purpose media used for nonfastidious microbes including many bacteria and fungi

This selective and differential media is used to isolate enteric bacteria that hydrolyze urea

Which of the following is commonly used to culture algae?

Which piece of glassware is used to sterilize media in?

The method uses media to separate out individual microbes to make pure culture is known as

The first step when removing a colony from a slant is

The first step in inoculating an agar plate is

Which tool is used to sterilize the inoculating loop?

Growing microbes in culture is important to increase visibility and manage microbes in an artificial environment is which of the following

This method takes a mixed culture and selects a single colony to result in a pure culture

This type of media can grow several types of microbes but are designed to bring out visible differences among those microbes

Which selective media uses methylene blue to inhibit the growth of non-target bacteria?

MSA is selective for which bacteria?

EMB agar is selective because only bacteria that ferments this sugar produce colored colonies

Colonies of E. coli on EMB agar will produce what color colonies?

In this phase, the cells reach the maximum rate of cell division

In this phase, there are few cells present

How many cells are present after 20 generations (starting from 1 cell) of exponential growth?

A dilution of 1mL of bacteria from broth into 999mL broth or water

A serial dilution of 1:10 followed by 1:10 gives a final dilution of

A serial dilution of 1:4 followed by 1:2 gives a final dilution of

A dilution of 0.1mL bacteria from broth in 999.9mL broth or water

A 1:1,000,000 is a common dilution factor, which serial dilution will end with this dilution?

Which generation time is calculated from 30 x log 2 / (log 1257 - log 536)?

Two samples have the following absorbance readings 0.23 (A) and 0.17 (B). Which one has more cells present?

The generation of cellular energy, ATP, can have metabolic reactions that require a particular amount of which physical property?

Microbes that are flexible and can grow with or without oxygen are

When using a fluid thioglycollate media which type of bacteria would be present at the top of the media?

Which bacteria have optimal growth between 45°C to 75°C?

When the temperature increases past the optimal, these denature

When the temperature increases past the optimal, lipids can be destroyed affecting which structure?

When the temperature increases past the optimal, the hydrogen bonding in the RNA breaks down and the proteins denature

A bacterium that grows at a pH of 4 is a

An environment that has more solutes than a cell is

These microbes can grow in moderate concentration of sodium chloride