magnification
increase in the apparent size of an object.
resolution
- clearness of an object.
arm
- supports body tube
base
supports microscope
ocular lens
- in eyepiece, magnifies 10x
body tube
connects nosepiece to eyepiece
revolving nosepiece
holds objectives
scanning power objective lens
sits over the specimen, magnifies image 4x
low power objective lens
sits over the specimen, magnifies image 10x
high power objective lens
sits over the specimen, magnifies image 40-100x
coarse adjustment knob
- moves body tube up and down, gross focus.
fine adjustment knob
- moves tube slightly, sharp focus
diaphragm
adjusts the amount of light passing through specimen
stage
- platform, where slide sits
stage clips
holds the slide in place
light source
bulb or mirror
compound light microscope
Uses a light source to pass through the specimen and an ocular and objective lens to magnify the image
can look at living organisms
magnifies up to 1000x
stereoscope
also called a dissecting microscope
can look at living organisms
uses combo of light and lens
better for 3D surface structures
magnifies 10's to 100'sx
Transmission Electron Microscope
TEM
transmits beam of electrons through the frozen, thinly sliced specimen (special preparation)
Good to look at the insides of cells, cell parts, etc.
magnifies up to 100,000x
Scanning Electron Microscope
SEM
- produces 3-D image when the surface is sprayed with a fine metal coating (gold) and the electron beam is passed over the surface.
- can look at viruses and other external structures
total magnification
the magnification of the ocular lens (10x) x the objective lens