parts of the microscope

cocci

round shape

diplococci

cocci that remain in pairs after dividing

streptococci

divides and remain attached in chainlike

tetrads

two planes and remain in groups of four

staphylococci

multiple planes. grapelike

bacilli

rodlike

single bacilli

single rods

diplobacilli

pairs after division

steptobacilli

chains

coccobacillus

oval like cocci

vibrio

curved rods. like a comma

spirillum

helical shape

spirochete

helical and flexible

spore staining

prepare slide

add culture

air dry (10min)

heat dry (5X)

put on noiling water

flood slide with malachite green

remove after 10mins and rinse with DI water

add safranin

wait 1min

perform microscopy

E.coli

gram negative

non acid fast

non sporulating

acid slant and acid butt

S. aureus

gram positive

non acid fast

non sporulating

bacillus

gram positive

acid fast

sporulating

M. smeg

acid fast

what test do you perform when a microbe is found to be catalase positive

coagulase test

DNAase

MSA

What happens to a medium when H₂O₂ is added to it

it bubbles which shows that it's catalase positive

which organisms came out to be catalase positive

S. aureus and S. epidermis

which organisms came out to be catalase negative

S.pyogenes

S. salvarius

S. fecalis

Which organism was MSA positive and what color did it turn

S. aureus; yellow

which organism was MSA negative and what color did it turn

S. epidermis; red

which organism had a clear halo after the DNAase test

S. aureus

which organism had no halo after the DNAase test

S. epidermis

Which organism clotted for the coagulase test

S. aureus

which organism didn't clot for the coagulase test

S. epidermis

Which organism turned black for the bile esculin test

S. fecalis

which organism turned green for the bile esculin test

S. pyogenes

S. salvaris

which organism turned yellow for the phenol red test

S. fecalis

which organism turned red for the phenol red test

s. pyogenes

S. salvarius

For the blood hemolysis test, which one was gamma

S. fecalis

which blood hemolysis came out to be beta

S. pyogenes

which blood hemolysis came out to be alpha

S. salvarius

what color does the organism turn when it's lactose fermenter

pink

what color does the organism turn when it's a non-lactose fermenter

colorless/white

what dye is first used in a gram staining

crystal violet followed by acetone decolorizer, then safranin

what dye is used in spore staining

malachite green

what was added to the TSI tubes

kovac's reagant

P. aer

pinkish

alkaline slant

alkaline butt

non lactose

P. vul

yellow slant

yellow butt

gas inside

black ppt

C. fir

black ppt

creates gas

yellow slant

K. pne

lactose fermenter

yellow everywhere

S. ent

black ppt only in acid

it turns the media yellow first

mordent for gram staining technique

iodine and moist heat

what dye is used as a counter stain

methylene blue

decolorizer for acid fast stain?

acid alcohol

decolorizer in gram staining

acetone alcohol