Those organisms that pose very little risk of disease for healthy students and they can still be capable of causing infection under certain circumstances are rated

BSL-1

Though we will not use these organisms during lab, these organisms can cause disease in healthy adults, pose a lethal risk, and do not respond to vaccines or antimicrobial therapies

BSL-4

Which container would you put a used coverslip in?

Broken glass container

Which sections of the SDS contain general information about the chemical, identification hazards, compositions, safe handling practices, and emergency control measures

1-8

Which machine is used to heat a mixture and stir a mixture using electromagnetic forces?

Hot plate/magnetic stirrer

Which piece of equipment measures the amount of material based on absorbance?

Spectrophotometer

Which tool is used to move microorganisms between cultures and can be repeatedly sterilized?

Inoculating loop

Which machine agitates a mixture in a test tube to make a homogenized mixture?

Vortex mixer

Which piece of glassware is used to move liquid culture around an agar plate evenly?

Spreader

Which pipet moves a fixed volume?

Pasteur piper

Which lenses are typically binocular?

Ocular lens

Which objective lens is used for quickly scanning the microscope slides?

4x

This type of microscope causes the light source to hit the specimen at a severely oblique angle

Darkfield

This is the bending of light as it passes from one medium to another

Refraction

Which one of the following phylum is considered an algae?

Rhodophyta

Which of the following is not in the group Alveolata?

Parabasilids

Which one of the following bacterial phyla is Gram positive?

Firmicutes

Which phylum contains the plant pathogenic fungi known as smuts and rusts?

Basidiomycota

Which phylum is an animal microbe?

Platyhelminthes

Which virus classification does not match the description?

Class VI- double stranded DNA with reverse transcriptase

Class I- double-stranded DNA

Class IV- single stranded RNA (+)

Which type of stain does not stick to the cell, but dries around the cell boundary creating a silhouette?

Negative staining

Which type of stain uses both basic and acidic dyes?

Positive staining

Which type of stain uses two dyes- a primary dye and a counterstain?

Differential staining

This type of differential stain reacts to a waxy material called mycolic acid in the bacterium's cell wall

Acid-fast staining

The method completed in lab using crystal violet and safranin

Gram staining

With Gram staining, Gram positive bacteria will stain as which color?

Purple/blue

Which spore staining, vegetative cells will appear as which color?

Red

With acid-fast staining method, non-acid-fast bacteria will stain which color?

Blue

The first step in smear preparation from a broth is?

Draw a "target circle" on the bottom of the microscope slide

Differential staining with an endospore stain _______ a heat-fixed smear

requires

These media contain agar for thickening but do not form a firm substrate

Semisolid

These media contain substances that absorb oxygen or slow the penetration of oxygen

Anaerobic growth

This type of media contains one or more agents that inhibit the growth of certain microbes but not another

Selective

This type of media has large molecules such as proteins, polysaccharides, and lipids that can vary greatly in exact composition

Complex media

When agar media is placed in a test tube and is allowed to solidify vertically, it is called a

Deep

This media is a general purpose media used for nonfastidious microbes including many bacteria and fungi

Nutrient Agar or both (NA or NB)

This selective and differential media is used to isolate enteric bacteria that hydrolyze urea

EMB

Which of the following is commonly used to culture algae?

Salt agar

Which piece of glassware is used to sterilize media in?

Media bottle

The method uses media to separate out individual microbes to make pure culture is known as

Isolation

The first step when removing a colony from a slant is

Flame an inoculating loop

The first step in inoculating an agar plate is

Open the lid of the petri dish

Which tool is used to sterilize the inoculating loop?

Micro-incinerator

Growing microbes in culture is important to increase visibility and manage microbes in an artificial environment is which of the following

Inoculation

This method takes a mixed culture and selects a single colony to result in a pure culture

Sub-culturing

This type of media can grow several types of microbes but are designed to bring out visible differences among those microbes

Differential

Which selective media uses methylene blue to inhibit the growth of non-target bacteria?

EMB

MSA is selective for which bacteria?

Staphylococcus sp.

EMB agar is selective because only bacteria that ferments this sugar produce colored colonies

Lactose

Colonies of E. coli on EMB agar will produce what color colonies?

Green

In this phase, the cells reach the maximum rate of cell division

Log phase

In this phase, there are few cells present

Lag phase

How many cells are present after 20 generations (starting from 1 cell) of exponential growth?

1,048,576

A dilution of 1mL of bacteria from broth into 999mL broth or water

1:1000

A serial dilution of 1:10 followed by 1:10 gives a final dilution of

1:100

A serial dilution of 1:4 followed by 1:2 gives a final dilution of

1:8

A dilution of 0.1mL bacteria from broth in 999.9mL broth or water

1:10,000

A 1:1,000,000 is a common dilution factor, which serial dilution will end with this dilution?

10^-6

Which generation time is calculated from 30 x log 2 / (log 1257 - log 536)?

24.4

Two samples have the following absorbance readings 0.23 (A) and 0.17 (B). Which one has more cells present?

A

The generation of cellular energy, ATP, can have metabolic reactions that require a particular amount of which physical property?

Oxygen levels

Microbes that are flexible and can grow with or without oxygen are

Facultative anaerobe

When using a fluid thioglycollate media which type of bacteria would be present at the top of the media?

Obligate aerobes

Which bacteria have optimal growth between 45°C to 75°C?

Thermophiles

When the temperature increases past the optimal, these denature

Enzymes

When the temperature increases past the optimal, lipids can be destroyed affecting which structure?

Cell membrane

When the temperature increases past the optimal, the hydrogen bonding in the RNA breaks down and the proteins denature

Ribosomes

A bacterium that grows at a pH of 4 is a

Acidophile

An environment that has more solutes than a cell is

Hypertonic

These microbes can grow in moderate concentration of sodium chloride

Halotolerant