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Microscopes

1.

magnification

increase in the apparent size of an object.

2.

resolution

  • clearness of an object.
3.

arm

  • supports body tube
4.

base

supports microscope

5.

ocular lens

  • in eyepiece, magnifies 10x
6.

body tube

connects nosepiece to eyepiece

7.

revolving nosepiece

holds objectives

8.

scanning power objective lens

sits over the specimen, magnifies image 4x

9.

low power objective lens

sits over the specimen, magnifies image 10x

10.

high power objective lens

sits over the specimen, magnifies image 40-100x

11.

coarse adjustment knob

  • moves body tube up and down, gross focus.
12.

fine adjustment knob

  • moves tube slightly, sharp focus
13.

diaphragm

adjusts the amount of light passing through specimen

14.

stage

  • platform, where slide sits
15.

stage clips

holds the slide in place

16.

light source

bulb or mirror

17.

compound light microscope

Uses a light source to pass through the specimen and an ocular and objective lens to magnify the image

can look at living organisms

magnifies up to 1000x

18.

stereoscope

also called a dissecting microscope

can look at living organisms

uses combo of light and lens

better for 3D surface structures

magnifies 10's to 100'sx

19.

Transmission Electron Microscope

TEM

transmits beam of electrons through the frozen, thinly sliced specimen (special preparation)

Good to look at the insides of cells, cell parts, etc.

magnifies up to 100,000x

20.

Scanning Electron Microscope

SEM

  • produces 3-D image when the surface is sprayed with a fine metal coating (gold) and the electron beam is passed over the surface.
  • can look at viruses and other external structures
21.

total magnification

the magnification of the ocular lens (10x) x the objective lens