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Molecular Biology

front 1

Steps to convert DNA sequence to amino acid sequence

back 1

-mRNA complementary to DNA (U replaces T)

- RNA assembled in 3'-5' direction in DNA transcribed 5'-3' direction in RNA

- divide into 3 letter codon 5'-3' direction

- find codon to identify 3 letter abbreviation for amino acid that corresponds to each codon

front 2

Translation

back 2

- performed by ribosomes of reading mRNA and a synthesizing protein

- RNA as amino acid and binds to codon of mRNA to tRNA

- initiation, the translation start codon of mRNA

- amino acid attached to tRNA by enzymes

front 3

Role of RNA in protein synthesis

back 3

Transcription - pre-mRNA, sn-RNA, mRNA

Translation - rRNA, tRNA

Not used - RNA primer

front 4

Purpose of cell cycle in mitosis

back 4

Create two genetically identical daughter cells from one parent cell

front 5

How cancer cells are related in mitosis

back 5

Cancer is the result of uncontrolled mitosis

front 6

What happens ONLY during S-Phase of interphase of mitosis

back 6

Chromosomes duplicate to form sister chromatids

front 7

Equation for 2 homologous pairs of chromosomes in mitosis

back 7

2n=2

front 8

Where does meiosis occur

back 8

Exclusively in reproductive organs

front 9

Mitosis of a diploid cell results in

back 9

Daughter nuclei that are also diploid

front 10

Roles of mRNA

back 10

- produced after steps of RNA processing

- template for protein synthesis

- carries genetic info from nucleus to cytoplasm

front 11

Translation

back 11

creates a polypeptide (sequence of amino acids) based on sequence of nucleotides in a mRNA molecule

front 12

Transcription

back 12

Create an mRNA molecule based on the sequence of nucleotides in a DNA molecule

front 13

Carbon Fixation

back 13

- Incorporation of carbon into inorganic compounds, begins with CO2 from air into organic molecules present in chloroplast

- Occurs in Calvin Cycle in stroma

front 14

Mutations

back 14

Changes in genetic material of a cell

front 15

Point Mutations

back 15

Chemical changes in just one base pair

front 16

Nucleotide Substitutions

back 16

- Silent

- Missense

- Nonsense

front 17

Nucleotide Insertion or Deletion - Frameshift Mutations

back 17

- Insertion results in nonsense

- Deletion results in missense

front 18

3 steps in translation

back 18

-Initiation - start codon binds with tRNA with amino acid MET

- Elongation - mRNA code is read and polypeptide built correctly

- Termination - stop codon binds to a release factor and a polypeptide is released

front 19

Steps in Elongation

back 19

- H-Bonds to peptide

- Peptide bonds to connect peptides

- mRNA shifts making more codons available to start cycle again

front 20

Function of tRNA and ribosomes

back 20

-tRNA brings specific amino acid to matching codon in the ribosomes

- ribosome translation happens polypeptide is form

front 21

How RNA is modified after transcription

back 21

- Info for coding is non-coded introns regions

- Exons for coding regions expressed

- Splicing takes out introns and connects exons together

front 22

Caps and Tails (AAA)

back 22

- Tail - adenine nucleotides at end forms a tail of RNA (AAA)

- 5' cap added begins transcription recognizing signal for ribosomes to bind to mRNA

- 3' end bind with AAA

front 23

General Role of Transcription and Translation

back 23

Transcription unit, segment of DNA to be transcribed into RNA

- Initiation - involved promotes TATA box and transcription factors

- Elongation - RNA polymerase adds nucleus to the 3' end of the growing molecule

- Termination - specific sequence of DNA signals transcription to stop

- RNA transcript is released

front 24

Role of Promoter and Terminator

back 24

- Starts with primer ends with terminator

- Poly A tails in transcription unit DNA sequence that signals transcription to translation AAA

front 25

How RNA polymerase recognized where transcription should begin

back 25

- RNA polymerase does not need any primers, does not require a promoter

- Promoter, beginning of gene, a specific nucleotide sequence nucleotide sequence nucleotide sequence that signals the transcription start point

- TATA box is an important promoter in eukaryotes

front 26

Codon

back 26

- Triplet code series of non-overlapping three nucleus words transcribes words of a gene

(Transcription)

front 27

Anaerobic Respiration

back 27

Catabolic pathway in which inorganic molecule other than oxygen accept electrons at the downhill end of the electron transport chain

front 28

G2 of Interphase

back 28

- Chromosomes duplicated, uncondensed

- Two centrosomes with two centrioles formed by duplication of a single centrosome regions that organize microtubules of spindle

- NE encloses nucleus

front 29

RNA processing

back 29

RNA polymerase are joined to one another by spliceosomes, exons are expressed, 5' cap consists of modified guanine nucleotide

front 30

Flow of Information

back 30

DNA-RNA-Protein

front 31

Role of amino acids in a poly chain

back 31

Three base sequence of mRNA

front 32

mRNA

back 32

Before molecule of mRNA can be translated into a protein on the ribosome, mRNA must be transcribed from sequence of DNA

front 33

Aerobic Respiration

back 33

Catabolic for organic molecule using O2 as the final electron acceptor in an electron transport chain, ultimately producing ATP, most efficient catabolic pathway used in most eukaryotic cells and many prokaryotic organisms

front 34

Light Reaction

back 34

- Occurs in the thylakoid

- Split H20 and releases O2

- reduce electron acceptor NADP+ to NADPH

- generate ATP from ADP by phosphorylation

- NAD+ accepts high energy electrons

front 35

Prophase

back 35

- Chromosomes begin to condense

- Duplicated

- Nucleus intact

- Coiling happens

- Mitotic Spindle forms

front 36

Telophase and Cytokinesis

back 36

- 2 daughter nuclei form

- chromosomes less condensed

- mitosis complete

- cytokinesis divides cell

- separate unduplicated chromosomes

front 37

Anaphase

back 37

- NO NE

- each chromosome becomes 2 daughter chromosomes

- cell begins to elongate

- microtubules shorten, pulling chromosomes toward opposite poles

front 38

Metaphase

back 38

- centrosomes at opposite end of cell

- microtubules now attached to kinetochores at each sister chromatin

- align down plate

front 39

Prometa Phase

back 39

- NE breaks up

- attaching to kinetochores

- most condensed form

- beginning to line up

- centrosomes move begin to connect

front 40

What occurs during Calvin Cycle

back 40

- CO2 is reduced

- ATP is hydrolyzed and NADPH is oxidized

- CO2 combines with 5 carbon compound

front 41

Energy used to produce ATP comes from

back 41

movement of H+ through membrane, chemiosmosis diffusion of H+ through ATP synthesis provides energy to produce ATP

front 42

NAD+ in photosynthesis

back 42

is reduced then carries electrons to the Calvin Cycle

front 43

Relation between Light Reaction and the Calvin Cycle

back 43

NADH provides reducing power by donating electrons and ATP provides energy which is converted to ADP

front 44

During Photosynthesis and Electrochemical Gradient

back 44

Is formed across the Thylakoid Membrane, in chloroplasts the electron transport chain pumps protons from the stroma to the thylakoid space, these locations are separated by thylakoid membranes

front 45

Input and Output of Carbon Fixation

back 45

Inputs - CO2, ATP, NADPH

Outputs- Sugar, ADP, NADPH+, Pi

Light dependent reactions of photosynthesis produce ATP and DANPH, which are then used in glucose synthesis during Calvin Cycle

front 46

Carbon Fixation

back 46

Cycle begins by incorporating CO2 from air into organic molecules already present in chloroplast. Calvin Cycle, then reduces the fixed carbon into carbs by the addition of electrons.

front 47

Histone

back 47

Highly alkaline proteins found in eukaryotic cells, nuclei that package and order the DNA into structural units calles nucleosomes

front 48

Fundamental differences between producers and consumers

back 48

Autotrophs, self feeders - sustain self without eating anything derived from other living beings, produce inorganic molecules from CO2 and other inorganic raw materials obtained from their environment - Producers

Heterotrophs - other feeders, rely on other organisms for organic carbon from which to build cells and obtain energy - Consumers, may eat producers, other consumers including dead producers or consumers

front 49

Calvin Cycle

back 49

Carbon enters cycles as CO2 and leaves as GP3, 3 revolutions to produce one G3P, uses all ATP and NADPH from light reaction, products are ADP and NADP+

front 50

Light Reaction

back 50

Converts light energy to chemical energy, uses ATP and NADPH splits H2O releases CO2 into atmosphere

front 51

Autrotroph

back 51

Self-feeders, producers, creates organic carbon molecules from inorganic CO2 and other inorganic starting materials

front 52

Meiosis II

back 52

All cells are haploid

front 53

Stomata

back 53

Microscopic pores in leaf, CO2 enters and O2 exits

front 54

Heterotrophs

back 54

Rely on other organisms for organic compounds, consumers

front 55

Chloroplast

back 55

Cellular organelle (plastid) where photosynthesis takes place

front 56

Mesophyll

back 56

Chloroplasts found mainly in cells, tissue in interior of leaf

front 57

Stroma

back 57

Chloroplast has 2 membranes surrounded in a dense fluid

front 58

Thylakoid

back 58

Connected sac in chloroplast which compose a 3rd membrane system, separates stroma from thylakoid space

front 59

Why plants are green

back 59

Pigments can only observe certain wavelengths of light, absorb blue and red the rest is reflected, we can see green, the rest of the visible light is reflected

front 60

Photosystems

back 60

Biochemical reaction in plants by which chlorophyll absorbs light energy for photosynthesis

front 61

Photophosphorylation

back 61

Synthesis of ATP from ADP and Pi occurs in a plant using radiant energy absorbed during photosynthesis

front 62

Pigments & Wavelenghs

back 62

Chlorophyll can only absorb certain wavelengths of lights

front 63

Importance of a cell division

back 63

Reproduction

Growth

Tissue Repair

front 64

Genome

back 64

Entirety of a cells genetic material

front 65

Chromosome

back 65

Packaged typically very long segment of DNA, involved proteins called histones in eukaryotes

front 66

Fermentation Vs Cellular Respiration

back 66

Both processes produce ATP for cell use

Fermentation does not use O2

front 67

How NADH is cycled back to NAD+

back 67

O2 is not present, pyruvate undergoes fermentation, NADH and H+ from glycolysis will be reduced back NAD+ so glycolysis can continue

front 68

NADH in fermentation and Cellular Respiration

back 68

Acts as an electron shuttle, when O2 not present

front 69

During photosynthesis

back 69

O2 is produced from H2O via a series of reactions associated with photosystem II

front 70

G2 of Interphase

back 70

Centrisomes with centriole pairs

NE

Nucleus

Chromosomes duplicated and uncondensed

front 71

Chromatin

back 71

Entire complex of DNA and protein that is the building of material of chromosome

front 72

Feedback Inhibition

back 72

Phenomenon where the output of a process is used as an input to control the behavior of the process itself, oftentimes limiting the production of more product. Although negative feedback is used in the context of inhibition, negative feedback may also be used for promoting a certain process.

front 73

Fate of CO2 in photosynthesis

back 73

Appear in sugar molecules and in H2O

front 74

Source of O2 in photosynthesis

back 74

H20, splitting H2O

front 75

Chlorophyll molecule are in which part of the chloroplast

back 75

Thylakoid Membranes, embedded

front 76

How does CO2 enter the leaf

back 76

Through stomata

front 77

In photosynthesis plants use carbon from ____________to make sugar and other organic moleclues

back 77

CO2

front 78

Stacks of thylakoids

back 78

In higher plants thylakoids are organized into a granum-stroma membrane assembly. A granum (plural grana) is a stack of thylakoid discs. Chloroplasts can have from 10 to 100 grana. Grana are connected by stroma thylakoids, also called intergranal thylakoids or lamellae.

front 79

Stomata

back 79

any of the minute pores in the epidermis of the leaf or stem of a plant, forming a slit of variable width that allows movement of gases in and out of the intercellular spaces

front 80

Mesophyll

back 80

Chloroplasts are found, tissue in the interior of the leaf

front 81

Kinetochure

back 81

Complex of proteins with the centromere of a chromosome during cell division to which the microtubules attach

front 82

Mitotic Spindle

back 82

Segregates chromosomes to 2 daughter cells during mitosis, major structural element of the spindle are microtubules

front 83

Centrosome

back 83

Organelle near nucleus of a cell that contains the centrioles in an animal cell and form which the spindle fibers develop in cell division

front 84

Centromere

back 84

Each sister chromatid has a centrosome region made up repetitive sequences in the chromosomal DNA where the chromatid is attached most closely to its sister chromatid

front 85

Photosystem

back 85

System is formed by reaction center, light harvesting complexes primarily electron acceptors that cluster located in thylakoid membrane

front 86

Sister Chromatid

back 86

Each duplicated chromosome consists of 2, which are joined copies of the original chromosome

front 87

When chloroplast absorb light

back 87

Electrons become excited, has more energy potential

front 88

Role of NAD+ in photosynthesis

back 88

Forms NAD+ to be used in Calvin Cycle

front 89

DNA polymerase

back 89

Adds nucleotides after Primase, attaches nucleotides to form DNA strand, replaces RNA with DNA

front 90

DNA Ligase

back 90

Enzyme joins fragments with sugar backbone to continuous DNA strand

front 91

Primase

back 91

Starts complimentary RNA chain, enzyme puts down primers as starting points for copying DNA

front 92

Lead and Lagging

back 92

Can't move in same direction, because moving in 5' - 3' direction fragments find primer synthesize, need a naked prime end

front 93

Topiosomerase

back 93

Protein, swivels breaks down and rejoins DNA strand

front 94

Lagging Strand

back 94

Made in fragments, okazaki, 5'-3' direction copying DNA, needs a naked 3' prime end

front 95

Helicase

back 95

Enzyme, that unzips double helix of DNA at replication fork

front 96

RNA Primer

back 96

Creates a 3' end start point, by primase drops on a naked 3' end for DNA Polymerase, duplication of DNA will begin here

front 97

Leading Strand

back 97

5'-3' direction of sequence of DNA being copied, new DNA copied

front 98

Origins of Replication

back 98

Short stretches of a sequence of DNA, begins at specific locations within chromosomes, where DNA is copied,

front 99

Role of tRNA

back 99

Major structural component of ribosomes, involved in binding of both RNA and tRNA

front 100

Role of tRNA

back 100

Implement genetic code, translates info from sequence of nucleotides to sequence of amino acid that make protein

front 101

Plants as producers

back 101

Produce inorganic CO2 into organic molecules

front 102

Missense

Nonsense

Silent

back 102

Causes wild type amino acid replicated with different amino acid

Stop codon

No change in wild type amino acid sequence

front 103

Frameshift mutations have severe effects because

back 103

Substitution missense affects only 1 codon, frameshift missense affects all codons downstream

front 104

A swaps with T

back 104

Silent or Missense

front 105

Anticodons on tRNA that binds mRNA codon

back 105

UUU

front 106

True of tRNA

back 106

transfer amino acid to growing polypeptide change, molecule involved in translation of mRNA

front 107

RIbozyme

back 107

biological catalyst made of RNA

front 108

Introns & Extrons

back 108

Introns are removed

Extrons are expressed

front 109

Functions of RNA polymerase

back 109

Unwinds double helix and adds nucleotides to growing strand of RNA

front 110

Where does RNA polymerase begin transcribing a gene into mRNA

back 110

after Promoter

front 111

Flow of information

back 111

DNA-RNA-Protein

front 112

Mutated DNA sequences produces a protein that differs in one central amino acid from normal protein

back 112

Addition or Deletion

front 113

1 addition and 1 deletion

back 113

would not change reading frame of gene sequence that follows mutation

front 114

codon specifies 1 amino acid

back 114

yes, only 1

front 115

Ribosomes are

back 115

found in cytoplasm, site of translation

front 116

RNA segments are joined by another spliceosomes

back 116

Exons, expressed regions

front 117

spliceosomes are composed of

back 117

small RNAs and proteins

front 118

During RNA processing _______________added to 3' end of RNA

back 118

Poly A tail added, long string of adenine

front 119

During RNA processing _______________added to 5' end of RNA

back 119

5' cap consists of guanamine

front 120

direction of synthesis of a RNA transcript

back 120

5'-3'

front 121

Promoter

back 121

region of DNA at which process of transcription begins

front 122

RNA polymerase

back 122

untwists a portion of DNA double helix

front 123

Codon sequence of __________ bases and specifies ____________will be installed into polypeptide chains

back 123

3....amino acids

front 124

RNA processing converts RNA transcript into

back 124

mRNA

front 125

polypeptide ares assembled from

back 125

amino acids, monomers

front 126

process which pre-mRNA is edited to mRNA

back 126

RNA processing

front 127

process which information in a strand of mRNA is used to construct protien

back 127

translation, info encoded in RNA is used to synthesize a polypeptide

front 128

in a nucleosome, DNA is wrapped around

back 128

histones

front 129

elongation of leading strand during DNA synthesis

back 129

depends on action of DNA polymerase

front 130

what holds DNA strands apart

back 130

single stranded DNA binding proteins

front 131

role of ligase in elongation of lagging strand

back 131

joins okasaki fragments

front 132

leading - lagging

back 132

leading strand synthesized in same direction as movement of fork

lagging strand opposite direction

front 133

old DNA strand is __________for assembly of a new DNA strand

back 133

template

front 134

primase

back 134

responsible for catalyzing formation of RNA primer

front 135

synthesis of a new strand begins with synthesis of

back 135

RNA primer complementary to a preexisting strand, synthesis of DNA stand begins with formation of RNA primer

front 136

after DNA replication

back 136

each new strand of DNA is double helix consists of one old DNA strand and one new DNA strand

front 137

short segments of newly synthesized DNA at joined into continuous strand by

back 137

ligase

front 138

an old DNA strand is used as a __________for the assembly of a new DNA strand

back 138

template

front 139

synthesis of a new strand begins with synthesis of a

back 139

RNA primer complementary to a preexisting strand

front 140

why is new DNA strand complimentary to 3'-5' strands assembled in short segments

back 140

DNA polymerase can assemble DNA only in 5'-3' direction

front 141

action of helix creates

back 141

replication forks and replication bubbles, fork is transition between paired and unpaired DNA strands

front 142

first step in replication of DNA is catalyzed by

back 142

helicase

front 143

after DNA replication

back 143

each new DNA double helix consists of one old and one new DNA strand

front 144

in DNA helix A pairs with _______ of complementary strand and G pairs with ________of complementary strand

back 144

T.....C, base pairing

front 145

single strand binding proteins

back 145

prevents H-Bonding, binds after replication fork

front 146

topiosomerase

back 146

binds ahead of replication fork, breaks covalent bonds in DNA backbone

front 147

helicase

back 147

breaks H-Bonds, binds at replication fork

front 148

chemical structure of DNA and its nucleotides

back 148

5'en H-Bond 3'end,

deoxyribose sugar, phosphate group in 5'end

nitrogenous base in 3'end

3' end - 5'end

front 149

materials DNA polymerase needs in order to synthesize a complete strand of DNA

back 149

all four deoxyribonucleotide triphosphates (A C T G)

single stranded DNA template

3' OH end of a new DNA strand

front 150

addition of nucleotide onto DNA strand is endergonic_________provides energy to drive reaction

back 150

release of pyrophosphate from incoming nucleotide, then hydrolysis of pyrophosphorate to inorganic phosphate

front 151

a hydroxyl is present at 3' end of growing DNA strand, what is present of 5'end

back 151

phosphate group, joins next deosyribonucleotide onto growing strand

front 152

role of DNA polymerase during synthesis of DNA

back 152

enzyme that catalyzes addition of nucleotide onto the 3' end of a growing DNA strand, using existing strand as a template

front 153

DNA content is halved during

back 153

Meiosis I & II

front 154

During meiosis II cells are

back 154

haploid

front 155

DNA is made of

back 155

genes

traits are heritable characterists,

front 156

chromosomes specifies position on locus

back 156

building material of chromatids, passed on in gametes, found in nucleus

front 157

sister chromatids separate from each other

back 157

during mitosis & meiosis II

front 158

what happens during meiosis but not mitosis

back 158

synapsis, pairing of homologous pairs of chromosomes only occurs during prophase I

front 159

mitosis results in formation of how many cells.....

meiosis results in formation of how many cells.......

back 159

2 diploid cells

4 haploid cells

front 160

a diploid cells whose somatic cells each contain 32 chromosomes produces gametes containing________chromosomes

back 160

16

front 161

at end of meiosis I

back 161

cells are haploid

front 162

normal human gametes carry________chromosomes

back 162

23

front 163

human gametes are formed by

back 163

meiosis

front 164

plant cells reproduce by

back 164

asexual reproduction

front 165

two sister chromatids are joined at centromere prior to meiosis

back 165

barring mutation, must be identical

front 166

chromosomes has 2 chromatids joined at centromere, what process led to this

back 166

formed by duplication of a chromosome, formation of chromatids

front 167

diploid cells

back 167

contain 2 sets of chromosomes

front 168

diploid cells

back 168

cell contains 2 copies of each chromosome 1 from each parent

front 169

recombinant chromosomes

back 169

each chromosome in gametes are composed of material derived form both parents

front 170

processes led to most genetic variation in sexually reproducing organisms

back 170

crossing over

random fertilization

independent assortment of chromosomes in meiosis

front 171

during________a spindle forms in a haploid cell

back 171

prophase I

front 172

at end of __________& cytokinesis there are 4 haploid cells

back 172

telophase II

front 173

during__________chromosomes align single file along equator of a haploid cell

back 173

metaphase II

front 174

homologous chromosomes migrate to opposite poles during

back 174

anaphase I

front 175

synapsis occurs during

back 175

prophase I

front 176

end of ____________& cytokinesis haploid cells contain chromosomes each consist of 2 sister chromatids

back 176

telophase I and cytokinesis

front 177

during________sister chromatids separate

back 177

anaphase II

front 178

meiosis II typically produces________each of which is___________

back 178

4...haploid

front 179

meiosis I produces___________cells each of which is

back 179

2......haploid

front 180

in mitosis, metaphase contains 20 sister chromatids, how many chromosomes will be present in G1 cell

back 180

10

front 181

in mitosis, diploid cell with 5 chromosome pairs (2n=10) how many sister chromatids will be found in nucleus of prophase

back 181

20

front 182

in mitosis, interphase is best described as what portion of the cell life

back 182

resting stage before cell division, accounts for 90% of cell cycle

front 183

cell a has 1/2 as much DNA as b, c, & d in a mitotically active tissue, cell a is most likely in

back 183

G1

front 184

in mitosis, cells line up on cell plate beginning to develop across the middle of cell and nuclei are forming on either side of cell plate

back 184

cytokinesis

front 185

in mitosis, m phase checkpoint ensures that all chromosomes are attached to the mitotic spindle, if this does not happen, cells would likely be arrested in

back 185

metaphase

front 186

in mitosis what happens during interphase

back 186

replication of DNA

front 187

in mitosis, during what phase do chromatids become chromosomes

back 187

anaphase

front 188

in mitosis metaphase is characterized by

back 188

alignment of chromosomes on the equator of the cell

front 189

in, mitosis name of microtubule organizing center in animal cells, as an identifiable structure present during all phases of cell life

back 189

centrosomes

front 190

in mitosis G1 is associate with

back 190

normal growth and development

front 191

in, mitosis what phase does the cell grow and replicate both its organelles and its chromosomes

back 191

interphase

front 192

in mitosis, both the contents of the nucleus and cytoplasm are divided

back 192

mitotic phase

front 193

in, mitosis during prophase a homologous pair of chromosomes consist of

back 193

2 chromosomes and 4 chromatids

front 194

in mitosis, spindle fibers attach to kinetochures during what phase

back 194

prometaphase

front 195

centromeres divide and sister chromatids become full-fledged during

back 195

anaphase

front 196

chromosomes become visible during what cycle in mitosis

back 196

prophase

front 197

cytokinesis often, but not always accompanies

back 197

telophase

front 198

what phase are nucleoli present in

back 198

interphase

front 199

eukaryotic chromosomes are composed of

back 199

DNA and protein

front 200

what organism did process of photosynthesis most likely originate

back 200

prokaryotes

front 201

what molecule is the main product of the calvin cycle

back 201

G3P and sugar

front 202

where does calvin cycle take place

back 202

stroma

front 203

why do leaves change color

back 203

other pigments such as carotenoids are still present in the leaves

front 204

function of light reaction of photosynthesis

back 204

conversion of solar energy to chemical energy

front 205

conditions photosynthesis and cellular respiration occur in most plants

back 205

photosynthesis only occurs in the light cycle

cellular respiration occurs only in the dark cycle

front 206

light reactions of photosynthesis use_______and produce___________

back 206

water.....NADPH in calvin cycle

front 207

outputs for photosynthesis

back 207

c6h12o6, 6O2

front 208

inputs for photosynthesis

back 208

CO2 , light energy, water

front 209

photosynthesis produces

back 209

O2, G3P