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Microbiology Exam 1

front 1

The lipopolysaccharide (LPS) that is found in the outer membrane of gram negative bacteria is also known as?

back 1

Murein

front 2

Bacteria are good models to use because they?

back 2

Share many biochemical/ physiological properties with more complicated organisms

front 3

Which shows the incorrect complementary base pairing?

back 3

G:T

front 4

The polynucleotide strands of DNA are linked along their lengths by _______ bonds.

back 4

Hydrogen

front 5

The N terminal in a protein

back 5

Is the end characterized by a free amino group

front 6

Acidic or basic amino acids are

back 6

Readibly soluble in water, able to form ions and considered hydrophilic

front 7

Which of the following is not true of bacterial plasmids?

back 7

They are required for host growth and/ or reproduction

front 8

Water?

back 8

Is polar

is referred to as a universal solvent

makes up 70% ( by wt) of an organism

is often a product or reactant in chemical reactions

front 9

The opposite results obtained by scientists apparently doing the same experiments in investigating spontaneous generation?

back 9

Shows the importance of repeating experiments

shows the importance of exactly duplicating experimental conditions

led to further experiments that ultimately furthered knowledge

front 10

A weak, attractive force between nearby molecules is called a ?

back 10

Hydrogen bond

front 11

Carbohydrates?

back 11

May interconvert between ring and linear structures

front 12

The microscope which allows the specimen to appear 3-dimensional is the?

back 12

Interference microscope

front 13

ATP is best described as?

back 13

The energy molecule of cells

front 14

Which of the following strains is/are considered differential?

back 14

Acid fast stain and gram stain

front 15

Bacteria and Archean ribosomes are known as _______ ribosomes, based on their sedimentation coefficient.

back 15

70s

front 16

The subunits ( building blocks) of proteins are?

back 16

Amino acids

front 17

Although penicillin inhibits peptidoglycan synthesis, bacterial cells will continue to grow normally in the presence of penicillin in an ________ environment.

back 17

Isotonic

front 18

A microbiologist obtained two pure isolated biological samples: one of the virus, and one a viroid . The labels came off during a move from one lab table to the next , however. The scientists felt she could distinguish between the two samples by analyzing for the presence of a single type of molecule. What type of molecule would she be looking for to differentiate between the two?

back 18

Protein

front 19

Amino acids in proteins are linked to one another by peptide bonds between the?

back 19

Carboxyl group of one amino acid and the amino group of another

front 20

Immunofluorescence ?

back 20

Uses fluorescently tagged molecules, makes use of the specificity in binding of antibodies and would require a special UV microscope

front 21

Which is true of simple diffusion of water?

back 21

Water usually enters a cell and produces a tremendous osmotic pressure and the diffusion ultimately relies on the selectively permeable nature of the cell membrane.

front 22

The sugars found in nuclei can acids consists of ?

back 22

5 carbon atoms

front 23

The purines of DNA are?

back 23

Adenine and guanine

front 24

Which may be or is an RNA molecule ?

back 24

GGGCCCA and GCCCUUA

front 25

The idea of spontaneous generation postulated that?

back 25

Living organisms could spontaneously arise from Non-living material

front 26

In general, when saturated fats are compared to unsaturated fats ( assuming the same number of carbon atoms in the molecule)

back 26

Unsaturated fats have a lower melting temperature

front 27

GCCCAAG is a molecule of?

back 27

Cannot tell as written

front 28

HIV/AIDS can be categorized as a new or emerging infectious disease. By putting it into a category , we are effectively saying that

back 28

This infection hasn't been observed in the human population prior to recent ( approximately years or so sooner) outbreaks

front 29

Which is not true about enzymes?

back 29

Have high-energy bonds between phosphates

front 30

What type of binding holds one strand of DNA to the complementary strand of DNA?

back 30

Hydrogen

front 31

Shrinkage of the plasma membrane away from the cell wall when the bacterium is placed in hypertonic environment is called?

back 31

Plasmolysis

front 32

All of the following pertain to 14 C except it?

6

back 32

Has 14 neutrons

front 33

In viewing a microscope specimen , oil is used to?

back 33

Decrease the refraction and increase the resolution

front 34

Prior to staining , smears of microorganisms are heat fixed in order to

back 34

Attach it firmly to the slide

front 35

Viruses are often referred to as ?

back 35

Infectious agents

front 36

Which of the following is found in RNA but not in DNA?

back 36

Ribose and Uracil

front 37

The cell type which lack a membrane bound nucleus are found in the ?

back 37

Prokaryotes and Archaea

front 38

Which is true of carbohydrates?

back 38

They may be part of the structure of bacteria

the may serve as a source of food

they contain carbon, hydrogen and oxygen in a 1:2:1 ratio

they may be bonded to proteins to form glycoproteins

front 39

Endospores are?

back 39

A dormant cell type

front 40

Penicillin would be most effective against ?

back 40

Growing bacteria and gram positive bacteria

front 41

The scientific name of an organism includes its?

back 41

Genes and species

front 42

The term antiparellel ?

back 42

Referees to the opposite orientation of the two strands in DNA

front 43

Proteins that are loosely attached to the cytoplasmic membrane are called?

back 43

Peripheral

front 44

The energy storage form of ATP?

back 44

Contains ribose

front 45

Endospores represents a challenge to the fields of industrial and medical microbiology because?

back 45

They are resistant to harsh environments, thus allowing survival of spore forming organisms under conditions in which nonsporulating cells would not survive and spore forming organisms are often dangerous pathogens are correct.

front 46

The valence number is the?

back 46

Number of outermost electron

front 47

Phagocytosis?

back 47

Is the ingestion of particles and may be performed by animal cells

front 48

Scientists recently cloned Luis Pasteur and put him back to work in the lab. He promptly developed a gel that breaks down proteins. Since he hasn't been around for some time; he's unsure what the best application for his invention might be. Help him out what pathogenic item would this gel be most effective and safe at eliminating?

back 48

Viruses on the surface of the skin

front 49

Viruses ?

back 49

Are obligate intracellular parasites

front 50

The twisted ladder shape of DNA is a result of the covalently bonded building blocks called?

back 50

Nucleotides

front 51

A research laboratory is investigating environmental factors that would inhibit growth of Archaea. One question they have is if adding the antibiotic penicillin would be effective in controlling their growth. What do you think the outcome would be if they tried this?

back 51

The penicillin wouldn't affect the archaea because it prevents crosslinking of peptidoglycan-Archaea don't have this compound in their cell walls

front 52

The system by which organisms are named is referred to as ?

back 52

Nomenclature

front 53

A monosaccharide with 5 carbon atoms will have __________ hydrogen atoms and ________ oxygen atoms.

back 53

10,5

front 54

Which group below contain single- celled and multicellular organisms?

back 54

Algae and fungi

front 55

The term used to describe bacteria that are rod shaped is?

back 55

Bacillus

front 56

If electrons are gained or lost in the formation of a bond, the bond is termed ?

back 56

Isotonic

front 57

The nucleus?

back 57

Is a double membrane sac containing DNA and is found in eukaryotes

front 58

A microscope that exposes specimens to ultraviolet, violet, or blue light and forms an image within the light emitted at a different wavelength is called?

back 58

Fluorescence

front 59

If while investigating spontaneous generation, Pasteur had his laboratory located in a stable?

back 59

The results would, most likely, have supported the idea of spontaneous generation

front 60

Which molecules are associated with the cell wall of gram positive bacteria?

back 60

Peptidoglycan, D- form amino acids and teichoic acids

front 61

Viruses may only be grown ?

back 61

In living cells

front 62

Which is not usually true of Archaea ?

back 62

They contain peptidoglycan as part of their cell walls

front 63

Rotating the following answers any way necessary, but not changing their sequence, which are complementary to the sequence 5' AGGCUAAC 3'?

back 63

3' TCCGATTC 5'

front 64

Endotoxin?

back 64

Consist of LPS

front 65

In the gram staining procedures, the primary stain us?

back 65

Crystal violet

front 66

A new drug is developed that targets and binds to the lipid A portion of LPS from gram negative bacterial cells. This drug shows a high degree of activity and binding in a test tube setting against purified lipid A. Based on this info?

back 66

We should next proceed by moving to testing in a test tube using whole gram negative cells to see if it binds with the same strength.

front 67

Bacteria are present on the body?

back 67

Constantly

front 68

Charged atoms are termed?

back 68

Ions

front 69

All of the following are monosaccharides except?

back 69

Glycogen

front 70

Extrachromasomal DNA is found in ________.

back 70

Mitochondria and plasmids

front 71

How many times more acidic is a solution with a pH of 3 than a solution with a pH of 6?

back 71

1000

front 72

The atomic number for an atom of a specific element is equal to?

back 72

The number of protons in a single atom of that element

front 73

The major criteria used in placing bacteria into different groups is based on differences in?

back 73

Cell wall structure

front 74

Bacteria are useful to study because?

back 74

They produce protein in a similar manner to more complex organisms

they replicate DNA in a similar manner to more complex organisms

they produce energy in a similar manner to more complex organisms

they are grown quickly, easily, and cheaply.

front 75

pH?

back 75

Is a measure of the hydrogen ion concentration

front 76

The components of fats are fatty acids and?

back 76

Glycerol

front 77

Ionic bonds?

back 77

Results from transferring electrons

front 78

Outside a cell, viruses are?

back 78

Inactive

front 79

The gram staining procedure is an example of?

back 79

Differential staining

front 80

The order of reagents in the gram stain reaction are?

back 80

Crystal violet, iodine, alcohol and safranin

front 81

Protein denaturation can?

back 81

Occur due to certain chemicals

occur due to pH changes

occur due to high temperature

cause the protein to no longer function

front 82

The helices and sheets of amino acids from a proteins ?

back 82

Secondary structure

front 83

Which is the correct form?

back 83

Staphylococcus aeurus and S. Aureus

front 84

Which of the following is useful in distinguishing between prokaryotic and eukaryotic cells?

back 84

The presence of a membrane-delimited organelles within the cytoplasm

front 85

Basic dyes?

back 85

Have positive charges

front 86

Atoms that gain electrons are now?

back 86

Negatively charged

front 87

Which of the following represents a synthesis reaction?

back 87

A+B ----> AB

front 88

The proteins of bacteria that are involved in the movement of small molecules into the cell, are called?

back 88

Transport proteins, permeases and carriers

front 89

The alpha helix and beta pleated sheet are examples of?

back 89

Secondary structures

front 90

The carbohydrates found in nucleic acids are?

back 90

Ribose and deoxyribose

front 91

The type of chemical bond linking amino acids together is a ?

back 91

Peptide bond

front 92

Which microscope would be the best selection for examination of a virus?

back 92

Scanning electron microscope

front 93

Cellulose is a major component of plants and is only directly digested by?

back 93

Microorganisms

front 94

The characteristics common to all lipids is their?

back 94

Solubility in organic solvents and hydrophobic nature

front 95

A 45X objective and a 10X ocular produce a total magnification of?

back 95

450X

front 96

The most important feature of a protein is its ?

back 96

Shape

front 97

Chemotaxis is a process by which bacteria?

back 97

Move toward an attractant or away from a repellent

front 98

The primary structure in a protein?

back 98

Refers to the sequence of amino acids

front 99

Endospores represent a challenge to the fields of industrial and medical microbiology?

back 99

Endospores are resistant to harsh environments, thus allowing survival of endospore forming organisms under conditions in which non endospore forming cells would not survive, and endospore forming organisms are often dangerous pathogens.

front 100

C6 H12 O6 + C6 H12 O6----> C12 H22 O11+ H20 represents?

back 100

Dehydration synthesis