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Micro - Exam 2

front 1

What is the asexual process by which a cell divides to form two new cells?

back 1

Binary Fission

front 2

The proper term for CYTOPLASMIC division is.....

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Cytokinesis

front 3

Referring to an organism that grows in the presence OR absence of oxygen gas.

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Facultative

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Referring to an organism that does not require free oxygen.

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Anaerobe

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A lab test in which a statistical evaluation is used to estimate the number of bacteria in a sample of fluid; often employed in determinations of coliform bacteria in water.

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Most Probably Number (MPN)

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Each bacterial cell on an agar growth medium that divides to produce a discrete colony.

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Colony Forming Unit (CFU)

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The time interval between bacterial divisions.

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Generation Time

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An organic substance that helps stabilize the proteins in a bacterial spore, therefore increasing spore resistance.

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Dipicolinic Acid

front 9

The cloudiness of a broth culture due to a bacterial growth.

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Turbidity

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The final portion of a bacterial growth curve in which environmental factors overwhelm the population and induce death; also called death phase.

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Decline Phase

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The portion of a bacterial growth curve in which the reproductive and death rates of cells are equal.

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Stationary Phase

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The portion of a bacterial growth curve during which active growth leads to a rapid rise in cell numbers.

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Llogarithmic Phase or Log Phase

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Referring to a prokaryotic cell requiring low oxygen gas and a high concentration of carbon dioxide gas for metabolism.

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Capnophilic

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A measure of the viable cells by counting the number of colonies on a plate; each colony presumably started from one viable cell.

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Colony Forming Unit

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A prokaryote living under extremely high temperature environments.

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Hyperthermophile

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The initial portion of a bacterial growth curve during which time the cells are preparing for reproduction (binary fission).

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Lag Phase

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A bacterium not inhibited by oxygen gas.

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Aerotolerant

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An accumulation or colony of microorganisms of one species.

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Pure Culture

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A chemically undefined medium in which the nature and quantity of each component has not been identified.

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Complex Medium

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A growth medium in which special nutrients are added to get a species to grow.

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Enriched Medium

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A microorganism that grows at acidic pH's below 4.

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Acidophile

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A procedure to estimate the number of cells in a sample dilution spread on an agar plate.

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Standard plate count

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A growth medium in which different species of microorganisms can be distinguished visually.

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Differential medium

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An organism that live in cold temperature ranges from 0-20 Celsius.

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Psychrophiles

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Referring to microorganisms that grow at 0*C but have a temperature optima of 20*C-40*C.

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Psychrotolarent

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Estimation of the number of cells by observation with the light microscope.

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Direct microscopic count.

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An extremely resistant dormant cell produced by some gram positive bacterial species.

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Endospores

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A process by which a mixed culture can be separated into pure colonies and the colonies isolated.

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Pour plate method

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Referring to an organism that grows best in an oxygen reduced environment.

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Microaerophile

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An archaeal organism living in an extreme environments.

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Extremophile

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A bacterial medium that binds oxygen gas from the atmosphere and creates an environment suitable for anaerobic growth.

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Thioglycollate broth

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A chemically defined medium in which the nature and quantity of each component is identified.

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Synthetic medium

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An organism that grows only in the absence of oxygen gas.

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Obligate Anaerobe

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A polysaccharide derived from marine seaweed that is used as a solidifying agent in many microbiological culture media

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Agar

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A common bacteriological growth medium consisting of beef extract, peptone, water and agar.

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Nutrient Agar

front 36

An archaeal organism living in an extremely acidic environment.

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Extreme halophile

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Referring to prokaryotes that are viable but not culturable.

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VBNC

front 38

An organism living in environments with high concentrations of salt.

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Halophile

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A process by which a mixed culture can be streaked onto an agar plate and pure colonies isolated.

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Streak Plate Method

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A growth medium that contains ingredients to inhibit certain microorganisms while encouraging the growth of others.

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Selective medium

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A microorganism that lives under conditions of high atmospheric pressure.

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Barophile

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The second portion of a bacterial growth curve, in which active growth leads to a rapid rise in cell numbers.

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Llogarithmic or Log phase

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A microbe that grows best without salt but can tolerate low concentrations.

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Halotolerant

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The process of spore formation.

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Sporulation

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An organism that does not require or cannot use oxygen gas for metabolism.

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Anaerobe

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An organism that cannot use oxygen gas for metabolism.

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Obligate anaerobe

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The time duration for a cell to double in number.

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Generation time

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A growth medium containing special nutrients used to grow fastidious organisms.

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Enriched medium

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A differential growth medium that encourages the growth of staphylococci by containing a high percentage of salt, which is inhibitory to most other microorganisms.

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Mannitol Salt Agar (MSA)

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A prokaryote that has an optimal growth temperature above 80*C.

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Hyperthermophile

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An organism that grows best in an oxygen reduced environment.

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Microanaerobe

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A microorganism that cannot grow in the presence of added sodium chloride

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Nonhalophile

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An organism that requires oxygen gas for metabolism.

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Obligate Aerobe

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An organism that lives in high temperature ranges of 40*C-90*C.

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Thermophile

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A chemically defined medium in which the components are not known.

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Complex medium

front 56

The form of reproduction that maintains genetic constancy while increasing cell number.

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Asexual reproduction

front 57

A bacterium not inhibited by oxygen gas

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Aerotolerant

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An organism that that grows in temperature ranges of 20*C-40*C.

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Mesophiles

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The production of ATP in the presence of an inorganic final electron acceptor other than oxygen gas.

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Anaerobic respiration

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The slowing down or prevention of a metabolic pathway when excess end product binds noncompetitively to an enzyme in the pathway.

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Feedback inhibition

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A cyclic series of enzyme-catalyzed reactions in which carbon Acetyl-CoA is released as carbon dioxide; the reactions also yield protons and high-energy electrons that are transported among coenzymes and cytochromes as their energy is released.

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Krebs cycle

front 62

A biochemical process in which light energy is converted to chemical energy and used in carbohydrate synthesis.

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Photosynthesis

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A biochemical process in which amino groups are enzymatically removed from amino acids or other organic compound.

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Deamination

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An anabolic, energy-requiring process.

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Endergonic reaction

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An organism that uses light energy to synthesize nutrients from carbon compounds other than carbon dioxide.

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Photoheterotroph

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An organic molecule that forms the nonprotein part of an enzyme molecule

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Coenzyme

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A metal ion that is needed for enzyme function.

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Cofactor

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An organism that derives energy from organic chemicals and uses the energy to synthesize carbon compounds other than carbon dioxide.

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Chemoheterotroph

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The substance(s) in which an enzyme acts.

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Substrate

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The sum of all biochemical processes taking place in a living cell.

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Metabolism

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The region of the enzyme where the substrate binds.

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Active site

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A pigmented molecule that functions in photosynthesis; exists free in the cytoplasm of prokaryotes and within the chloroplasts of eukaryotes.

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Chlorophyll

front 73

The production of ATP using the organic compound Pyruvate as the final electron receptor.

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Fermentation

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The use of a proton gradient across a membrane to generate cellular energy in the form of ATP.

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Chemiosmosis

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A six-carbon sugar used as a major energy source for metabolism.

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Glucose

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A type of heterotrophic organism that feeds on dead organic matter, such as rotting wood or compost.

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Saprobe

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A pigment located in the membrane systems of purple sulfur bacteria that upon excitement by light, loses electrons and initiates photosynthetic reactions.

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Bacteriochlorophyll

front 78

The inhibition of enzyme function through the binding of a molecule to a non-active site on the enzyme.

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Noncompetitive Inhibition

front 79

A chemical process releasing energy

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Exergonic

front 80

A series of enzyme-catalyzed chemical reactions in which glucose is broken down into two molecules of pyruvate with a net gain of 2 ATP molecules.

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Glycolysis

front 81

The gain of electrons by a molecule.

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Reduction

front 82

A chemical change in which electrons are lost by an atom.

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Oxidation

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A type of heterotrophic organism that feeds on live organic matter such as another organism.

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Parasite

front 84

An organism that uses light energy to synthesize nutrients from carbon dioxide.

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Photoautotroph

front 85

A form of photosynthesis in which molecular oxygen is produced.

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Oxygenic Photosynthesis

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A group of pigments that act as a light trapping system for photosynthesis

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Photosystem

front 87

A substance(s) resulting from a chemical reaction

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