Print Options

Card layout: ?

← Back to notecard set|Easy Notecards home page

Instructions for Side by Side Printing
  1. Print the notecards
  2. Fold each page in half along the solid vertical line
  3. Cut out the notecards by cutting along each horizontal dotted line
  4. Optional: Glue, tape or staple the ends of each notecard together
  1. Verify Front of pages is selected for Viewing and print the front of the notecards
  2. Select Back of pages for Viewing and print the back of the notecards
    NOTE: Since the back of the pages are printed in reverse order (last page is printed first), keep the pages in the same order as they were after Step 1. Also, be sure to feed the pages in the same direction as you did in Step 1.
  3. Cut out the notecards by cutting along each horizontal and vertical dotted line
To print: Ctrl+PPrint as a list

21 notecards = 6 pages (4 cards per page)

Viewing:

Lab

front 1

Identify and give the function of the microscope. Condenser, iris diaphragm, objective lens, ocular lens, fine-coarse focus adjustment

back 1

front 2

Terms to Understand: Resolution, depth of field, working distance, illumination

back 2

Resolution:Ability to see details of object

Depth of field: Vertical distance through which the object is in focus

Working Distance:Between the objective and the slide.

Illumination: lighting

front 3

Be able to calculate the total magnification of a compound light microscope.

back 3

no data

front 4

Know the function of immersion oil

back 4

Function of immersion oil is to prevent refraction (loss) of light

front 5

Estimate the size of an object in a microscope if you know the diameter of the field.

back 5

Example: To determine the field of view for a 40x objective (total magnification 400x) insert the values for the 10x objective (total magnification of 100x)

(100) x (2mm)= (400)x (Y)= 200mm=400Y

200mm/400 = Y

.5=Y

front 6

Calculate how to prepare any volume of any culture medium, given the amount of dehydrated power used to prepare one liter.

back 6

no data

front 7

Describe a colonys shape, size, optical properties, margin, elevation, color

back 7

front 8

Use a drawing of a petri dish and words to describe how to prepare a streak plate.

back 8

1) Aseptically load the loop once

2) streak, using a tight zig zag pattern in the first "quadrant" on SM agar plate

3) flame loop to sterilize it, can use portion of agar to cool loop

4) Reload, overlapping streaks into the 1st section

5)Repeat #3, 4 by overlapping 4-5 times into the second quadrant

6) Repeat #3, 4 by overlapping into the 3rd quadrant

front 9

Describe the proper orientation of petri dishes in the incubator and refrigerator.

back 9

Incubator: Inverted

front 10

why are smears heat fixed?

back 10

You heat fix the bacteria to kill it and make it adhear to the slide

front 11

What is the difference between simple and differential stains?

back 11

Simple stains:

Positive stain: dye with positive charge on colored part attached to cell wall with color. Ex: crystal violet, methylene blue, safranin

Negative Stain: dye with negative charge on colored part leaves cells unstained, background colors Ex: congo red, nigrosin

Differential stains:

Use of 2 or may dyes

After satining, one group of cells are one color; a different type of cell has a different color

Example: Gram += purple; Gram -=pink

front 12

Select the proper sequence for making smears from solid and liquid media.

back 12

no data

front 13

What are the color of gram positive and gram negative organisms?

back 13

Gram Positive: Purple

Gram Negative: Pink

front 14

What are the reagents, in order of use, in the gram stain?

back 14

Gram's Crystal Violet, Grams Iodine, Acetone/alcohol, Gram Safrainin (Come In And Stain)

front 15

What are the functions of the reagents?

back 15

Gram Crystal Violet- Primary stain

Gram Iodine- a mordant that increases affinity between the dye and cell wall

Acetone/alcohol- decolorizing agent

Gram's safranin- secondary stain

front 16

Why do some organisms stain unevenly in terms of gram positiveness?

back 16

no data

front 17

What are the common genera of the endospore producing bacteria?

back 17

Prokaryotic

front 18

What are the colors of the spores and cells in the methods (Schaeffer-Fulton & Gram Stain) used to stain endospores forming bacteria?

back 18

Spores stain green while the vegetative cells are red.

front 19

What is the purpose of using heat during the endospore staining process?

back 19

Heat drives the stain into the cells.

front 20

What are two advantages of using staining rather than positive staining?

back 20

no data

front 21

How does negative staining differ from positive staining?

back 21

Positive stain: dye with positive charge on colored part attached to cell wall with color. Ex: crystal violet, methylene blue, safranin

Negative Stain: dye with negative charge on colored part leaves cells unstained, background colors Ex: congo red, nigrosin