30 notecards = 8 pages (4 cards per page)
General (Complex) Medium
Contains necessary nutrients, vitamins, minerals, etc. Most types of non-fastidious organisms, but not all, are able to grow on this type of media.
Contains added ingredients such as blood, serum, vitamins, growth factors, etc.
Contains an indicator designed to cause certain organisms to develop a different appearance from other microbes growing on the same medium. The colonies of one particular bacteria might be a different color than those of another bacterial species growing on the same medium.
Permits or encourages the growth of certain organisms while preventing or inhibiting the growth of others.
Many of the media commonly used in the lab possess both selective and differential qualities.
Eosin Methylene Blue Agar
Clear plate with iodine added after growth
Mannitol Salt Agar
Opaque red plate
Blood Agar- Alpha hemolysis
Partial destruction, greenish agar (S. salivarius)
Blood Agar- Beta hemolysis
Total destruction, clear zone (Strep. pyogenes)
Blood Agar- Gamma hemolysis
Negative no change(Staph. epidermidis)
Hektoen Enteric Agar
Dark Blue Plate
Phenol Red Carbohydrate Fermentation Broth
Red Tube with durham tube
Triple Sugar Iron Agar
Red slant tube, contains glucose, lactose, sucrose.
Triple Sugar Iron Agar (positive fermentation of only glucose)
red slant, yellow butt
Triple Sugar Iron Agar (positive fermentation of glucose, lactose, and/or sucrose plus positive gas formation.)
Citrate Agar Slants
Green slant tube
Clear to yellowish slant
SIM Medium (Semisolid)With positive Hydrogen Sulfide
SIM Medium (Semisolid)With positive motility
+ motility -- growth diffuses away from stab line (Enterobacter)
SIM Medium (Semisolid) with positive indole production
+ indole (after adding Kovac's reagent) -- pink or red ring
Motility GI Medium/ TTC (MOT) with positive motility
Optichin Disk Test
Red plate painted with white circle (optichin)