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The Prokaryotes: Domains Bacteria and Archaea

front 1

Which system has five kingdoms made up of Monera (procaryotes); Protista (eukaryotic, unicellular); Fungi (eukaryotic, multicellular, chitin); Plantae (eukaryotic, multicellular) and Animalia (eukaryotic, multicellular)?

back 1

Whittaker's Five-Kingdom System

front 2

What is Woese's Three Domains based on?

back 2

Comparison of sequence of ribosomal RNA (rRNA)

front 3

The three domains are above the kingdoms. Name the three domains.

back 3

Eubacteria (prokaryotic and contain bacteria)

Archaea (Prokaryotic and contain archaeabacteria)

Eucarya (contains all eukaryotic kingdoms)

front 4

How many volumes are contained in Bergey's Manual 1st Edition? Name them.

back 4

Four volumes: I. Gram negative

II. Gram positive

III. Other Gram negative bacteria

IV. Actinomycetes and other Gram positive bacteria

front 5

What are the division of the volumes in Bergey's Manual 1st edition based on?

back 5

Gram reaction

cell shape

cell arrangement

Oxygen requirements

motility

metabolic properties

front 6

How many volumes are contained in Bergey's Manual 2nd edition? Name them.

back 6

Five volumes I. Archaea and deeply branching phototrophic bacteria

II. The Proteobacteria (Gram negative)

III. The Firmicutes (Gram positive)

IV. Odd bacteria

V. Actinobacteria

front 7

What does Low G+C mean?

back 7

Less G and C in the DNA

front 8

What does High G+C mean?

back 8

More G and C in the DNA

front 9

What are the groups included in Proteobacteria?

back 9

Alpha

Beta

Gamma

Delta

Epsilon

front 10

True or False. The firmicutes are gram negative.

back 10

False

front 11

True or False. The proteobacteria are gram negative

back 11

True

front 12

What is the phylogeny of proteobacteria based on?

back 12

Ribosomal RNA studies

front 13

Which of the proteobacteria are nitrogen-fixing of which the most common is Rhizobium?

back 13

Alpha

front 14

What industrial benefit does the alpha proteobacteria offer?

back 14

They produce acetic acid from ethyl alcohol and help to make vinegar

Acetobacter

Gluconobacter

front 15

What are obligate intercellular parasites?

back 15

Bacteria that must live inside the cell of the host

front 16

How are alpha proteobacteria medically important?

back 16

Rickettsia, Arthropod-borne, spotted fever

R. prowazekii - Epidemic typhus (Lice on humans)

R. typhi - Endemic murine typhus (fleas on rats)

R. rickettsii - Rocky Mountain Spotted Fever (Ticks)

front 17

Give an example of bacteria - vector - disease in the alpha proteobacteria group.

back 17

Ehrlichia - Tick-borne - ehrlichiosis

front 18

What cures most patients with alpha proteobacteria infections?

back 18

Doxycyxcline

front 19

What is the best way to prevent typhus?

back 19

Inoculation with the typhus vaccine series before traveling to endemic areas and to avoid contact with lice.

front 20

True or False. All proteobacteria is not gram negative.

back 20

False

front 21

Alpha proteobacteria are chemoautotrophic. What does it mean?

back 21

They oxidize nitrogen for energy (lose electron or breakdown CO2)

Fix CO2 - carbon source

front 22

What is the alpha proteobacteria plant pathogen and what does it do?

back 22

Agrobacterium - insert a plasmid into plant cells, inducing a tumor

front 23

The Beta proteobacteria are medically important and very dangerous. Why?

back 23

Bordetella are chemoheterotrophic and rod-shaped - B. pertussis causes whooping cough

Burkholderia are nosocomial infections - B. cepacia is used for pesticides and dangerous for cystic fibrosis

front 24

Neisseria is a beta proteobacteria - which diseases are attributed to this bacteria?

back 24

N. meningitidis

N. gonorrhoeae

front 25

What is the Gram status and shape is Neisseria?

back 25

Gram negative cocci

front 26

Which two bacteria will never be used in the lab due to its dangerous nature?

back 26

Neisseria - beta proteobacteria

Pseudomonas - gamma proteobacteria

front 27

When it comes to metabolism - how does alpha proteobacteria differ from beta proteobacteria?

back 27

Alpha are chemoautotrophic and beta are chemoheterotrophic

front 28

What is the largest subgroup of proteobacteria?

back 28

Gamma

front 29

The enterics are included in the gamma proteobacteria - what are they?

back 29

Located in the intestines and colon and all are facultatively anaerobic and have peritrichous flagella. Includes: Klebsiella

Proteus

Salmonella

Serratia

front 30

Which gamma proteobacteria is considered very dangerous due to being an opportunistic pathogen? Why?

back 30

Pseudomonas because they can change their genome to use any food product. Found in the burn unit; green in color and smells like grapes.

front 31

Which of the pseudomonas are nitrogen fixing?

back 31

Azotobacter and azomonas

front 32

Which gamma proteobacteria causes pneumonia and where are they found?

back 32

Legionellales - in streams, warm-water pipes and cooling towers

front 33

Which gamma proteobacteria are found in salt water or raw fish?

back 33

Vibrionales

front 34

Which disease is vibrionales known to cause?

back 34

Cholera and gastroenteritis

front 35

What is another very dangerous gamma proteobacteria that require heme fraction and NAD cofactor?

back 35

Haemophilus

front 36

Which type of media has to be used with Haemophilus proteobacteria?

back 36

Blood agar (TSA + 5% agar) that has been boiled causing the release of X factor -referred to as chocolate agar due to its dark color

front 37

Which type of media does Neisseria require?

back 37

TSA + 5% agar

front 38

Which bacteria infections are caused by Haemophilus influenzae?

back 38

Meningitis, pneumonia, bronchitis, septic arthritis, earaches

front 39

Which proteobacteria are microaerphilic with helical or vibrioid rods and motile by means of flagella?

back 39

Epsilon proteobacteria

front 40

Which of the epsilon proteobacteria have one polar flagellum and are gastroenteritis?

back 40

Campylobacter

front 41

Which epsilon proteobacteria causes spontaneous abortion in domestic animals?

back 41

C. fetus

front 42

Which epsilon proteobacteria is the leading cause of baterial diarrhea?

back 42

C. jejuni

front 43

Which epsilon probacteria causes heartburn, peptic ulcers and stomach cancer?

back 43

Helicobacter or helicobacter pylori

front 44

Which group are low G + C and gram positive?

back 44

Firmicutes

front 45

Which of the firmicutes are endospore-producing and obligate anaerobes?

back 45

Clostridium

front 46

Which of the firmicutes are endospore-producing rods and Bacillus?

back 46

Bacillales

front 47

Which of the bacillales are cocci?

back 47

Staphylococcus

front 48

Which are generally aerotolerant anaerobes and lack an electron transport chain?

back 48

Lactobacillales

front 49

What are some typical lactobacillales?

back 49

Lactobacillus (in yogurt)

Streptococcus

Enterococcus

Listeria (gram positive rod) - if contracted in pregnancy, 40% chance of miscarriage

front 50

Which group are not contagious, wall-less and pleomorphic?

back 50

Mycoplasmatales

front 51

Which bacteria causes "walking" pneumonia?

back 51

M. pneumoniae

front 52

What are the gram positive and high G + C called?

back 52

Actinobacteria

front 53

Which of that actinobacteria are normal flora in the throat?

back 53

Corynebacterium

front 54

Corynebacterium causes diphtheria and strep throat but how are they visually different?

back 54

In diphtheria, there is a gray layer of infection in the throat and in strep throat, there is a yellow layer of infection.

front 55

Which of the nonproteobacteria gram negative bacteria are oxygenic photoautotrophs?

back 55

Cyanobacteria

front 56

What are the basic characteristics of cyanobacteria?

back 56

oxygenic photosynthesis

gliding motility

fix nitrogen

front 57

What type of photosynthesis requires no oxygen?

back 57

Anoxygenic photosynthesis which is purple and green photosynthetic bacteria

front 58

Which nonproteobacteria gram negative bacteria is the longest gram negative bacteria?

back 58

Fusobacteria

front 59

Which group do spirochaetes and bacteriodetes fall under?

back 59

nonproteobacteria gram negative

front 60

Which of the bacteriodetes is found in the mouth and large intestine?

back 60

Bacteroides