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Exercise 2-2 Micro Lab: Colony Morphology

front 1 How does a colony form?	back 1 When a single bacterial cell is deposited on a solid nutrient medium, it begins to divide. One cell makes two, two makes four, four make eight. Eventually a visible mass of cells, a colony, appears.
front 2 In what 4 ways are color, size, shape, and texture of microbial growth determined?	back 2 1. genetic makeup of the organism 2. nutrient availability 3. temperature 4. incubation
front 3 The 5 basic categories of colony morphology	back 3 1. colony shape 2. margin (edge) 3. elevation 4. texture 5. pigment production (color)
front 4 3 types of colony shape	back 4 1. round 2. irregular 3. punctiform (tiny, pinpoint)

front 5 5 types of margin	back 5 1. entire (smooth with no irregularities) 2. undulate (wavy) 3. lobate (lobed) 4. filamentous 5. rhizoid (branched like roots)
front 6 entire	back 6 smooth with no irregularities
front 7 undulate	back 7 wavy
front 8 lobate	back 8 lobed

front 9 rhizoid	back 9 branched like roots
front 10 5 elevations of colonies	back 10 1. flat 2. raised 3. convex 4. pulvinate (very convex) 5. umbonate (raised in the center)
front 11 pulvinate	back 11 very convex
front 12 umbonate	back 12 raised in the center

front 13 3 types of texture	back 13 1. moist 2. mucoid 3. dry
front 14 4 types of pigment production (color)	back 14 1. opaque 2. translucent 3. shiny 4. dull
front 15 colony counter	back 15 used to view subtle differences in colony shape and size
front 16 what 2 things allow greater observation of detail in a colony counter?	back 16 1. transmitted light 2. magnifying glass

front 17 colony counter is best determined with ______.	back 17 reflected light
front 18 The grid in the colony counter background is a ______.	back 18 counting aid
front 19 6 colonies studied in this lab	back 19 1. micrococcus luteus 2. corynebacterium xerosis 3. lactobacillus plantarum 4. mycobacterium smegmatis 5. bacillus subtilis 6. proteus miabilis
front 20 Name these elevations, left to right	back 20 1. raised 2. raised, spreading edge 3. flat, raised margin 4. growth into medium

front 21 Name these elevations, left to right	back 21 1. convex 2. umbonate 3. plateau 4. flat
front 22 Name these margins, left to right	back 22 1. smooth, entire 2. rhizoid 3. irregular (erose) 4. lobate 5. filamentous
front 23 3 types of descriptions used to describe colonial morphology:	back 23 1. color 2. surface characteristics 3. consistency 4. optical properties
front 24 2 types of surface characteristics	back 24 1. dull 2. shiny

front 25 3 types of consistency	back 25 1. dry 2. butyrous-buttery 3. moist
front 26 2 types of optical properties	back 26 1. opaque 2. translucent
front 27 What bacteria is this? What is the color, shape, elevation and margin?	back 27 Staphylococcus epidermidis Color - white Shape - circular Elevation - raised Margin - entire
front 28 What bacteria is this? What 3 features that describe it?	back 28 Providencia stuartii 1. shiny 2. buff 3. convex

front 29 What bacteria is this? What 3 features that describe it?	back 29 Klebsiella pneumoniae 1. mucoid 2. raised 3. shiny
front 30 What bacteria is this? What 3 features that describe it?	back 30 Chromobacterium violaceum 1. shiny 2. purple 3. convex
front 31 What bacteria is this? What is the color, shape, elevation and margin of it?	back 31 Enterococcus faecium Color - white Shape - circular Elevation - Convex Margin - Entire
front 32 What is this? 5 features of the colonies	back 32 A) Bacillus cereus 2) Bacillus anthracis 1. dull 2. dry 3. raised 4. rough-textured 5. gray

front 33 What is being shown in these two views? What bacteria is this? What are the 3 features of this bacteria?	back 33 A) Alcaligenes Faecalis side view showing a raised center B) Alcaligenes Faecalis showing spreading edge 1. umbonate 2. opaque center 3. spreading edge
front 34 What is being shown here?	back 34 Filamentous growth
front 35 What bacteria is being shown in A and B and what are the differences between them? What 2 features of the bacteria are being showed here? Where is this bacteria found?	back 35 A) Clostridium sporaogenes grown on sheep blood agar and viewed with reflected light B) Clostridium sporaogenes grown on nutrient agar and viewed with transmitted light 1. irregular 2. rhizoid It is found in soil
front 36 _____ is an opportunistic pathogen	back 36 Staphylococcus epidermidis

front 37 ______ is a frequent isolate in urine samples obtained from hospitalized and catheterized patients	back 37 Providencia stuartii
front 38 ______ is found in human and animal feces	back 38 Enterococcus faecium
front 39 What bacteria is this? What does this growth demonstrate?	back 39 Alcaligenes faecalis Demonstrates spreading attributable to motility and is translucent
front 40 _______ is found in soil and water and rarely produces infections in humans	back 40 Chromobacterium violaceum

front 41 What are these slides showing? What bacteria is this?	back 41 The effect of age on colony morphology after 24 and 48 hours of growth Bacillus subtilis A) After 24 hours B) After 48 hours (note worm like appearance)
front 42 What bacteria is this? This bacteria produces colonies with what 2 features?	back 42 Bacillus subtilis 1. raised margin 2. dull surface
front 43 what bacteria is this? what feature of this colony is being shown here?	back 43 Mycobacterium smegmatis punctiform
front 44 What bacteria is this? In A, what 4 features of the bacteria are exhibited? What is being shown here in B?	back 44 Coynebacterium xerosis In A: 1. round 2. dull 3. buff 4. convex In B: circular

front 45 What bacteria is this? What is being demonstrated in this dish?	back 45 Erwinia amylovora the irregular shape and spreading edges
front 46 _________ is a plant pathogen.	back 46 Erwinia amylovora
front 47 What bacteria is shown here? What is this slide demonstrating?	back 47 Proteus vulgaris swarming growth pattern due to bacteria motility
front 48 What bacteria is shown here? What is being demonstrated here?	back 48 Pseudomonas aeruginosa Mucoid texture

front 49 _____________ is found in soil and water and can cause infections in burn patients	back 49 Pseudomonas aeruginosa
front 50 What are these samples showing?	back 50 Mixed soil cultures and diversity
front 51 What bacteria is this showing? How do we know?	back 51 Staphylococcus aureus White growth on blood agar plate demonstrating B-hemolysis is characteristic of staph. In A) the agar is darkening from the hemolysis. In B) there is weak hemolysis.
front 52 What bacteria is this?	back 52 Chromobacterium violaceum

front 53 What bacteria is on the left? the right? What is being demonstrated here?	back 53 Left - Micrococcus luteus Right - Kocuria rosea Pigment production in similar species of bacteria
front 54 What bacteria is this? What is being shown here with the encircled sections?	back 54 Serratia marcescens the influence of age on pigment production after 24 and 48 hours
front 55 What bacteria is being shown here? What is being demonstrated here?	back 55 Serratia marcescens influence of temperature on pigment production when grown in 37 degrees C on left and 25 degrees C on right
front 56 What bacteria is being shown here? What is being demonstrated here?	back 56 Chromobacterium violaceum The influence of nutrient availability on pigment production. The nutrient agar (right) has less nutrients so less pigment.

front 57

What bacteria is shown here?

What is being demonstrated here?

back 57

Pseudomonas aeruginosa

diffusible blue-green pigment