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Biochem 28

front 1

A healthy adult is 34 hours into fasting. Liver glycogen is depleted. What now maintains blood glucose?
A. Muscle glycogen breakdown
B. Hepatic glycogenolysis
C. Hepatic gluconeogenesis
D. Intestinal glucose absorption

back 1

C. Hepatic gluconeogenesis

front 2

Gluconeogenesis is glucose synthesis primarily from:
A. Noncarbohydrate precursors
B. Stored liver glycogen
C. Dietary polysaccharides
D. Ketone bodies only

back 2

A. Noncarbohydrate precursors

front 3

In humans, gluconeogenesis occurs primarily in:
A. Skeletal muscle cytosol
B. Brain astrocytes mainly
C. Adipose tissue mainly
D. Liver hepatocytes mainly

back 3

D. Liver hepatocytes mainly

front 4

Which is a major gluconeogenic precursor?
A. Palmitate
B. Lactate
C. Cholesterol
D. Acetyl-CoA

back 4

B. Lactate

front 5

The amino acid highlighted as a key gluconeogenic precursor is:
A. Alanine
B. Leucine
C. Lysine
D. Isoleucine

back 5

A. Alanine

front 6

Relative to glycolysis, gluconeogenesis requires:
A. No unique reactions
B. Only one bypass step
C. Complete enzyme replacement
D. Three bypass reactions

back 6

D. Three bypass reactions

front 7

After stopping food intake, liver glycogen breakdown begins to support blood glucose after about:
A. 15 minutes
B. 2–3 hours
C. 12–18 hours
D. 3–5 days

back 7

B. 2–3 hours

front 8

During an overnight fast, blood glucose is maintained mainly by:
A. Glycolysis plus lipogenesis
B. Ketolysis plus proteolysis
C. Glycogenolysis plus gluconeogenesis
D. Glycogenesis plus lipolysis

back 8

C. Glycogenolysis plus gluconeogenesis

front 9

As glycogen stores fall, adipose triacylglycerol breakdown provides:
A. Fatty acids and glycerol
B. Glucose and lactate
C. Ketones and glucose
D. Pyruvate and alanine

back 9

A. Fatty acids and glycerol

front 10

During fasting, fatty acids primarily help by:
A. Converting directly to glucose
B. Providing alternative fuel
C. Becoming DHAP in liver
D. Becoming lactate for liver

back 10

B. Providing alternative fuel

front 11

After ~30 hours of fasting, the only source of blood glucose is:
A. Muscle glycogenolysis
B. Dietary absorption
C. Hepatic glycogenolysis
D. Hepatic gluconeogenesis

back 11

D. Hepatic gluconeogenesis

front 12

Under extreme starvation, an additional site of gluconeogenesis is the:
A. Pancreatic islets
B. Renal medulla
C. Renal cortex
D. Splenic red pulp

back 12

C. Renal cortex

front 13

In extreme starvation, glucose produced by the kidney is mainly used by the:
A. Cerebral cortex neurons
B. Renal medulla cells
C. Hepatic stellate cells
D. Skeletal myocytes

back 13

B. Renal medulla cells

front 14

Most gluconeogenic steps mirror glycolysis, but overall:
A. Carbon flow is unchanged
B. Carbon flow is cyclic
C. Carbon flow is forward
D. Carbon flow is reversed

back 14

D. Carbon flow is reversed

front 15

Glycerol carbons are gluconeogenic because they form:
A. Dihydroxyacetone phosphate
B. Acetyl-CoA carbons
C. Citrate cycle intermediate
D. Oxaloacetate intermediate

back 15

A. Dihydroxyacetone phosphate

front 16

A hepatocyte uses glycolytic intermediates to generate glycerol 3-phosphate. Its key role is:
A. Acetyl-CoA backbone
B. Lactate backbone
C. Triacylglycerol backbone
D. Ketone body backbone

back 16

C. Triacylglycerol backbone

front 17

Liver triacylglycerols are secreted into blood primarily in:
A. HDL
B. LDL
C. Chylomicrons
D. VLDL

back 17

D. VLDL

front 18

Measuring ketones in blood and urine can indicate:
A. Starvation severity or DKA
B. Hyperthyroidism severity
C. Chronic liver failure only
D. SIADH severity

back 18

A. Starvation severity or DKA

front 19

A comatose patient has “fruity” breath. In DKA, the odor is due to:
A. Lactate
B. Acetone
C. Ammonia
D. Ethanol

back 19

B. Acetone

front 20

The “acetone” breath odor is linked to breakdown of which ketone body?
A. β-hydroxybutyrate
B. Propionyl-CoA
C. Acetoacetate
D. Oxaloacetate

back 20

C. Acetoacetate

front 21

Deep, relatively rapid respirations in DKA are termed:
A. Kussmaul respirations
B. Cheyne–Stokes breathing
C. Biot respirations
D. Apneustic breathing

back 21

A. Kussmaul respirations

front 22

Kussmaul respirations occur primarily because:
A. Hypoglycemia stimulates pons
B. Hypoxemia stimulates carotids
C. Fever stimulates medulla
D. Acidosis stimulates respiratory center

back 22

D. Acidosis stimulates respiratory center

front 23

The key gas exhaled more during Kussmaul compensation is:
A. HCO3−
B. CO2
C. Ketones
D. NH4+

back 23

B. CO2

front 24

Which paired finding most supports DKA coma over hypoglycemic coma?
A. Sweating, tremor
B. Confusion, hunger
C. Acetone breath, Kussmaul
D. Pallor, diaphoresis

back 24

C. Acetone breath, Kussmaul

front 25

Severe hyperglycemia in DKA causes polyuria mainly via:
A. Osmotic diuresis
B. SIADH effect
C. Nephrotic syndrome
D. Primary polydipsia

back 25

A. Osmotic diuresis

front 26

Volume depletion from DKA is commonly worsened by:
A. Polyphagia
B. Night sweats
C. Hemoptysis
D. Vomiting

back 26

D. Vomiting

front 27

A patient with DKA is volume depleted. Which hemodynamic profile fits best?
A. Hypertension, bradycardia, edema
B. Hypertension, bounding pulses, edema
C. Dehydration, hypotension, tachycardia
D. Bradycardia, hypothermia, pallor

back 27

C. Dehydration, hypotension, tachycardia

front 28

Why can ethanol carbons not support gluconeogenesis?
A. Ethanol forms glucose directly
B. Produces only acetyl-CoA
C. Produces only oxaloacetate
D. Produces only DHAP

back 28

B. Produces only acetyl-CoA

front 29

In liver, lactate and alanine contribute to gluconeogenesis by forming:
A. Pyruvate
B. Citrate
C. Palmitate
D. Cholesterol

back 29

A. Pyruvate

front 30

Which triacylglycerol component contributes carbon to gluconeogenesis?
A. Fatty acids
B. Sterol esters
C. Glycerol
D. Ketone bodies

back 30

C. Glycerol

front 31

A patient on a plant-heavy diet oxidizes an odd-chain fatty acid. The three ω-end carbons produce:
A. Acetyl-CoA
B. Succinyl-CoA
C. Propionyl-CoA
D. Pyruvate

back 31

C. Propionyl-CoA

front 32

Odd-chain fatty acids are obtained mainly from:
A. Vegetables
B. Dairy fat
C. Red meat
D. Fish oils

back 32

A. Vegetables

front 33

Propionyl-CoA is converted first to:
A. Succinyl-CoA
B. Acetoacetyl-CoA
C. Malonyl-CoA
D. Methylmalonyl-CoA

back 33

D. Methylmalonyl-CoA

front 34

Methylmalonyl-CoA is rearranged to:
A. Oxaloacetate
B. Succinyl-CoA
C. Malate
D. Citrate

back 34

B. Succinyl-CoA

front 35

In odd-chain fatty acid oxidation, net glucose can be made only from:
A. ω-end three carbons
B. Even-chain carbons
C. Acetyl-CoA carbons
D. Middle-chain carbons

back 35

A. ω-end three carbons

front 36

Why can acetyl-CoA not generate pyruvate for gluconeogenesis?
A. Pyruvate kinase is irreversible
B. PEPCK is irreversible
C. PDH reaction is irreversible
D. LDH blocks pyruvate formation

back 36

C. PDH reaction is irreversible

front 37

Which TCA reactions release the two CO2 that prevent net glucose from acetyl-CoA?
A. Citrate synthase, fumarase
B. Isocitrate DH, α-KGDH
C. Malate DH, aconitase
D. Succinate DH, thiolase

back 37

B. Isocitrate DH, α-KGDH

front 38

There is no net glucose synthesis from acetyl-CoA because:
A. Acetyl-CoA cannot enter TCA
B. OAA is fully consumed
C. NADH is always limiting
D. Two CO2 lost per turn

back 38

D. Two CO2 lost per turn

front 39

A 19-carbon fatty acid is oxidized. How many carbons form propionyl-CoA?
A. One
B. Two
C. Three
D. Four

back 39

C. Three

front 40

In a 19-carbon fatty acid, the remaining 16 carbons primarily form:
A. Acetyl-CoA
B. Pyruvate
C. Lactate
D. Glucose

back 40

A. Acetyl-CoA

front 41

In glycolysis, phosphoenolpyruvate is converted to pyruvate by:
A. PEP carboxykinase
B. Pyruvate kinase
C. Pyruvate carboxylase
D. Lactate dehydrogenase

back 41

B. Pyruvate kinase

front 42

Pyruvate carboxylase converts pyruvate to:
A. Citrate
B. Lactate
C. Acetyl-CoA
D. Oxaloacetate

back 42

D. Oxaloacetate

front 43

The enzyme that releases CO2 from OAA while generating PEP is:
A. PEP carboxykinase
B. Pyruvate kinase
C. Hexokinase
D. Citrate synthase

back 43

A. PEP carboxykinase

front 44

Oxaloacetate does not readily cross the:
A. Nuclear membrane
B. Lysosomal membrane
C. Mitochondrial membrane
D. Plasma membrane

back 44

C. Mitochondrial membrane

front 45

Because OAA cannot cross easily, it is often converted to:
A. Citrate or succinate
B. Glucose or glycogen
C. Acetyl-CoA or ketones
D. Malate or aspartate

back 45

D. Malate or aspartate

front 46

Glycerol enters gluconeogenesis at the level of:
A. Glucose 6-phosphate
B. DHAP
C. Citrate
D. Acetyl-CoA

back 46

B. DHAP

front 47

During rapid hepatic ethanol oxidation, the redox state shifts so:
A. NAD+/NADH increases
B. NADPH/NADP+ increases
C. NADH/NAD+ increases
D. FADH2/FAD increases

back 47

C. NADH/NAD+ increases

front 48

Elevated NADH inhibits glycerol use for gluconeogenesis because conversion to DHAP requires:
A. NAD+
B. ATP
C. Biotin
D. FAD

back 48

A. NAD+

front 49

High NADH drives lactate dehydrogenase toward producing:
A. Pyruvate
B. Oxaloacetate
C. Acetyl-CoA
D. Lactate

back 49

D. Lactate

front 50

After heavy alcohol intake, pyruvate generated from alanine is preferentially converted to:
A. Acetyl-CoA
B. Lactate
C. Citrate
D. Glucose

back 50

B. Lactate

front 51

A fasting patient binges alcohol and becomes hypoglycemic. Which precursors are least usable for gluconeogenesis?
A. Lactate, alanine, glycerol
B. Fatty acids, ketones, glycerol
C. Lactate, acetyl-CoA, ketones
D. Alanine, cholesterol, acetate

back 51

A. Lactate, alanine, glycerol

front 52

With high NADH, OAA is preferentially converted to:
A. PEP
B. Citrate
C. Malate
D. Succinyl-CoA

back 52

C. Malate

front 53

With high NADH, DHAP is preferentially converted to:
A. Pyruvate
B. Glycerol 3-phosphate
C. Lactate
D. Oxaloacetate

back 53

B. Glycerol 3-phosphate

front 54

A malnourished patient drinks heavily and then develops confusion and diaphoresis. Most likely metabolic outcome:
A. Hypernatremia
B. Metabolic alkalosis
C. Hyperglycemia
D. Hypoglycemia

back 54

D. Hypoglycemia

front 55

Fructose 1,6-bisphosphatase produces:
A. Fructose 1-phosphate
B. Fructose 6-phosphate
C. Fructose 2,6-bisphosphate
D. Glucose 1-phosphate

back 55

B. Fructose 6-phosphate

front 56

Glucose 6-phosphatase directly generates:
A. Glycogen
B. Glucose 6-phosphate
C. Lactate
D. Free glucose

back 56

D. Free glucose

front 57

Glucose 6-phosphatase is located in the:
A. Endoplasmic reticulum membrane
B. Mitochondrial matrix
C. Cytosolic ribosome
D. Nuclear envelope

back 57

A. Endoplasmic reticulum membrane

front 58

Which condition does NOT stimulate gluconeogenesis?
A. Prolonged exercise
B. High-protein diet
C. High-carbohydrate meal
D. Physiologic stress

back 58

C. High-carbohydrate meal

front 59

Which conversion is a regulated gluconeogenic “bypass”?
A. Lactate to pyruvate
B. PEP to pyruvate
C. Pyruvate to PEP
D. Glucose to G6P

back 59

C. Pyruvate to PEP

front 60

Which conversion is a regulated gluconeogenic “bypass”?
A. F1,6BP to F6P
B. F6P to F1,6BP
C. DHAP to G3P
D. G1P to glycogen

back 60

A. F1,6BP to F6P

front 61

Which conversion is a regulated gluconeogenic “bypass”?
A. Glucose to G6P
B. G6P to glycogen
C. F6P to F1,6BP
D. G6P to glucose

back 61

D. G6P to glucose

front 62

Hepatocytes should funnel PEP toward glucose during fasting. Which enzyme-state set best fits?
A. PDH↑ PC↓ PEPCK↓ PK↑
B. PDH↓ PC↑ PEPCK↑ PK↓
C. PDH↑ PC↑ PEPCK↓ PK↓
D. PDH↓ PC↓ PEPCK↑ PK↑

back 62

B. PDH↓ PC↑ PEPCK↑ PK↓

front 63

During conditions favoring gluconeogenesis, pyruvate dehydrogenase is typically:
A. Inactive
B. Active
C. Induced
D. Cleaved

back 63

A. Inactive

front 64

A fasting liver increases gluconeogenic capacity by inducing which enzyme quantity?
A. Pyruvate kinase
B. Phosphofructokinase-1
C. Hexokinase
D. PEP carboxykinase

back 64

D. PEP carboxykinase

front 65

A patient receives high-dose prednisone for vasculitis. Which lab abnormality is most expected?
A. Low blood glucose
B. High blood glucose
C. Low serum ketones
D. High serum lactate

back 65

B. High blood glucose

front 66

A fasting liver activates fructose 1,6-bisphosphatase. Which pair inhibits it allosterically?
A. Citrate and ATP
B. Glucose and insulin
C. F2,6BP and AMP
D. Pyruvate and NADH

back 66

C. F2,6BP and AMP

front 67

During fasting, hepatic ATP/NADH used for gluconeogenesis comes mainly from:
A. β-oxidation
B. Glycolysis
C. Glycogenesis
D. PPP flux

back 67

A. β-oxidation

front 68

An infant with a fatty-acid oxidation defect develops fasting hypoglycemia. Best mechanistic link?
A. Low hepatic glycogen
B. Low renal glucose
C. High insulin levels
D. Low hepatic energy

back 68

D. Low hepatic energy

front 69

Thirty minutes after a high-carbohydrate meal, glucose rises most typically to:
A. 65–75 mg/dL
B. 80–100 mg/dL
C. 120–140 mg/dL
D. 160–180 mg/dL

back 69

C. 120–140 mg/dL

front 70

After a typical meal, blood glucose returns to fasting range by about:
A. 30 minutes
B. 2 hours
C. 6 hours
D. 12 hours

back 70

B. 2 hours

front 71

A patient with severe hyperglycemia becomes bounded without ketones. Hyperosmolar coma stems mainly from:
A. Cerebral edema
B. Cerebral hemorrhage
C. Hepatic encephalopathy
D. Brain dehydration

back 71

D. Brain dehydration

front 72

About 2 hours after a meal, as glucose nears 80–100 mg/dL, the liver activates:
A. Glycogenolysis
B. Ketogenesis
C. Lipogenesis
D. Glycolysis

back 72

A. Glycogenolysis

front 73

After 5–6 weeks of starvation, blood glucose is closest to:
A. 90 mg/dL
B. 65 mg/dL
C. 45 mg/dL
D. 120 mg/dL

back 73

B. 65 mg/dL

front 74

Glucagon signaling in liver most directly raises intracellular:
A. IP3
B. DAG
C. cAMP
D. cGMP

back 74

C. cAMP

front 75

Glucagon raises hepatic cAMP by activating:
A. Protein kinase C
B. Guanylate cyclase
C. Tyrosine kinase
D. Adenylate cyclase

back 75

D. Adenylate cyclase

front 76

By ~4 hours after a meal, hepatic glucose output relies on glycogenolysis plus:
A. Gluconeogenesis
B. Glycolysis
C. Lipogenesis
D. Ketolysis

back 76

A. Gluconeogenesis

front 77

Glucagon prevents PEP from becoming pyruvate mainly by inactivating:
A. PEP carboxykinase
B. Pyruvate carboxylase
C. Pyruvate kinase
D. Lactate dehydrogenase

back 77

C. Pyruvate kinase

front 78

With pyruvate kinase inactivated, PEP “runs backward” to form:
A. Glucose 1-phosphate
B. Fructose 1,6-bisP
C. Acetyl-CoA
D. Lactate

back 78

B. Fructose 1,6-bisP

front 79

Enzymes unique to gluconeogenesis are most active under:
A. Fasting conditions
B. Postprandial conditions
C. After carbohydrate loading
D. During insulin surges

back 79

A. Fasting conditions

front 80

In fasting liver, acetyl-CoA from fatty acids directly activates:
A. Hexokinase
B. Pyruvate kinase
C. PFK-1
D. Pyruvate carboxylase

back 80

D. Pyruvate carboxylase

front 81

Which set is induced during fasting gluconeogenesis?
A. PFK-1, PK, LDH
B. PEPCK, FBPase, G6Pase
C. HK, PDH, CS
D. GCK, GS, GP

back 81

B. PEPCK, FBPase, G6Pase

front 82

Fructose 1,6-bisphosphatase is favored in fasting because:
A. AMP falls sharply
B. Citrate is absent
C. F2,6BP is low
D. NADH is low

back 82

C. F2,6BP is low

front 83

A carbon source explicitly used for gluconeogenesis is:
A. Glycerol
B. Palmitate
C. Cholesterol
D. Acetoacetate

back 83

A. Glycerol

front 84

During prolonged fasting, fatty acids are oxidized by tissues primarily to:
A. Lactate and ATP
B. Ketones and CO2
C. Glucose and ATP
D. CO2 and H2O

back 84

D. CO2 and H2O

front 85

After several days without food, the brain decreases glucose use by increasing:
A. Amino acid oxidation
B. Fatty acid oxidation
C. Ketone body use
D. Glycerol uptake

back 85

C. Ketone body use

front 86

After ~3–5 days fasting, brain glucose need is roughly:
A. Twice normal
B. One-third normal
C. Unchanged
D. One-tenth normal

back 86

B. One-third normal

front 87

Chronic hyperglycemia narrows microvessels mainly via protein:
A. Dephosphorylation
B. Proteolysis
C. Ubiquitination
D. Cross-linking

back 87

D. Cross-linking

front 88

Microvascular narrowing from chronic hyperglycemia classically targets:
A. Retina, glomeruli, nerves
B. Lung, marrow, spleen
C. Liver, skin, pancreas
D. Heart, brain, muscle

back 88

A. Retina, glomeruli, nerves

front 89

Narrowed renal glomerular microvessels most directly produce diabetic:
A. Retinopathy
B. Nephropathy
C. Neuropathy
D. Cardiomyopathy

back 89

B. Nephropathy

front 90

Narrowed microvessels to peripheral/autonomic nerves most directly produce diabetic:
A. Nephropathy
B. Retinopathy
C. Neuropathy
D. Dermopathy

back 90

C. Neuropathy

front 91

Fasting glucose 80–100 mg/dL is approximately:
A. ~2 mM
B. ~5 mM
C. ~8 mM
D. ~12 mM

back 91

B. ~5 mM

front 92

Post-meal glucose 120–140 mg/dL is approximately:
A. ~2 mM
B. ~5 mM
C. ~12 mM
D. ~8 mM

back 92

D. ~8 mM

front 93

Hepatic fructose 2,6-bisP levels are regulated by:
A. Insulin and glucagon
B. Cortisol and insulin
C. Epinephrine and ADH
D. Growth hormone and T3

back 93

A. Insulin and glucagon

front 94

After ~3–5 days fasting, brain glucose need is about:
A. 120 g/day
B. 80 g/day
C. 40 g/day
D. 10 g/day

back 94

C. 40 g/day