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79 notecards = 20 pages (4 cards per page)

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Micro Lab Final

front 1

What is the tool used for spreading plates?

back 1

Glass spreader or hockey stick

front 2

What do we use to inoculate semi-solid media

back 2

inoculation needle

front 3

Bacteria used in bacteriophage lab

back 3

Streptomyces greseus

front 4

How do you calculate titer/VCN?

back 4

(# of colonies x dilution factor)/mL pipetted

front 5

What is a plaque and how are they formed?

back 5

A plaque is a clearing in a bacterial lawn caused by a bacteriophage eating the nutrients on the agar and reproducing

front 6

What type of plaques are formed in the lysogenic and lytic cycle?

back 6

Lysogenic produces cloudy plaques whereas lytic produces clear plaques

front 7

Define coliphage and bacteriophage

back 7

coliphage: a bacteriophage that specifically infects e.coli

bacteriophage: a virus that infects bacteria

front 8

What does PFU stand for?

back 8

Plaque forming units

front 9

What is an obligate intracellular parasite?

back 9

an organism that can only reproduce inside of a host cell

front 10

lytic cycle stages

back 10

attachment, penetration, biosynthesis, maturation, and lysis

front 11

Are coliforms gram negative or positive and what do they ferment?

back 11

Gram negative and lactose fermenting rods

front 12

Two advantages of colisure test

back 12

Quick results: in under 24 hours

color change: easy to interpret

front 13

What is the principle indicator organism in fecal contamination

back 13

e.coli

front 14

wavelength of light used to interpret colisure test

back 14

365 nm

front 15

Name of enzyme in coliforms (all)?

back 15

B-Galactosidase

front 16

Name of enzyme in e.coli?

back 16

B-Glucuronidase

front 17

Principle behind oxidase test

back 17

Prescence of enzyme cytochrome c oxidase

front 18

Name of indicator in developing oxidase test

back 18

TMPD

front 19

What organism tested positive for the oxidase test

back 19

Pseudomonas Vibrioionaceae

front 20

What tested negative for oxidase test

back 20

Enterobacteriae

front 21

Review Rhizopus on plate

back 21

Black and tan and fuzzy

front 22

Review Aspergillus on plate

back 22

Solid black plate with little white

front 23

Media used for growing fungi in lab

back 23

SDA: sabouraud dextrose agar

front 24

define dimorphic fungi

back 24

produce anitbiotics ie.penicillium produces penicillin

front 25

What is lichen? How do they help each other?

back 25

Algae and fungi engaging in a symbiotic relationship

Algae provides carbohydrates and fungi provides structure, moisture, and protection

front 26

Enzyme that detoxifies hydrogen peroxide how do you test for it

back 26

Catalase

bubbles

front 27

What organism was negative for catalase test? positive?

back 27

n= streptococcus species

p= staphlyococcus aureus

front 28

What reagent was used for oxidase test

back 28

Hydrogen peroxide

front 29

Two stages of protozoa

back 29

Trophozoite: active, feeding, motile

Cyst: dormant, resistant stage

front 30

Two characteristics of protozoa

back 30

Unicellular eukaryotes

motile

front 31

4 classes of protozoa

back 31

Mastigophora: motile using flagella

Ciliophora: motile using cilia

Sarcodina: move using pseudopodia

Apicomplexa: non motile:glide

front 32

What reagent in used in developing starch hydrolysis test

back 32

grams iodine

front 33

Principle behind starch hydrolysis test

back 33

Produce amylase to break down starch

front 34

Example of mesophile

back 34

e.coli

front 35

example of thermophile

back 35

s. stearothermophile

front 36

what is a pandemic and epidemic

back 36

Worldwide spread

epi= increased number of cases in a specific area

front 37

Pathogen in epidemiology experiment

back 37

pseudomonas putida

front 38

MMWR?

back 38

Morbidity and mortality weekly report from the CDC

front 39

p. putida requires what to grow?

back 39

toluic acid

front 40

What is a pilus? what is a plasmid?

back 40

pilus: initiates conjugation by attaching to recipient cell

plasmid: small circular dna molecule that replicates independently of chromosomal dna

front 41

plasmid responsible for degrading toluic acid

back 41

TOL plasmid

front 42

back 42

Fusarium: fungi

front 43

back 43

Rhizopus stolonifer: fungi

front 44

back 44

Penicillium: fungi

front 45

back 45

Aspergillus: fungi

front 46

back 46

Saccaromyces: fungi

front 47

back 47

Penicillium fungi

front 48

back 48

Aspergillus niger fungi

front 49

back 49

Rhizopus stolonifer fungi

front 50

back 50

Saccaromyces cervisiae fungi

front 51

back 51

Fusarium fungi

front 52

back 52

chlamyldomonas algae

front 53

back 53

Spyrogira algae

front 54

back 54

Volvox algae

front 55

back 55

Diatoms algae

front 56

back 56

Desmids algae

front 57

back 57

anabaena cyanobacteria

front 58

back 58

Geleocapsa Cyanobacteria

front 59

back 59

Oscillatoria cyanobacteria

front 60

back 60

Spirulina cyanobacteria

front 61

back 61

Paramecium protozoan

front 62

back 62

Blepharisma protozoan

front 63

back 63

Vorticella protozoan

front 64

back 64

amoeba proto

front 65

back 65

Euglena proto

front 66

back 66

Stentor proto

front 67

back 67

trypanosoma proto

front 68

back 68

Plasmodium (ring stage) proto

front 69

back 69

Giardia Lambia

front 70

back 70

Trichomonas vaginillis proto

front 71

auxotroph v prototroph

back 71

auxotroph: mutant that cannot synthesize a required nutrient

prototroph: wild-type organism that can synthesize all required nutrients

front 72

One use of algae in lab

back 72

Agar

front 73

Study of algae is called

back 73

phycology

front 74

two modes of action for antibiotics

back 74

inhibiting cell wall synthesis and inhibiting bacterial protein growth

front 75

Name of antibiotic susceptibility test? name of media?

back 75

Kirby bauer test and the media was mueller hinton agar

front 76

advantage of using e-test over kirby bauer test?

back 76

Gives exact MIC (minimum inhibtory concentration)

front 77

difference between antiseptic and disinfectant?

back 77

Antispectic is for living skin, disinfectant is for inanimate surfaces

front 78

Structure of importance within fungi

back 78

septate hyphae

front 79

What is the name of the red pigment produced by serratia marcescens

back 79

Prodigiosin