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Microbiology Exam 3

front 1

Catabolism

back 1

Involves the breakdown of complex organic molecules to extract energy and form simpler end products

front 2

Anabolism

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uses the energy to synthesize necessary macromolecules and cell structures from simple precursors

front 3

Substrate

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Promotes a reaction by serving as a physical site upon which the reactant molecules, can be positioned for various interactions

front 4

Why can vitamin deficiencies prevent the complete holoenzyme from forming?

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Because the most important components of coenzymes are vitamins.

front 5

Thermal Energy

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From molecular motion

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Radiant Energy

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From visible light or other rays

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Electrical energy

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From a flow of electrons

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Mechanical energy

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From a physical change in position

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Atomic energy

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From reactions in the nucleus of an atom

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Chemical energy

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Present in the bonds of molecules

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Three main catabolic pathways of aerobic respiration

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Glycolysis, Kreb's Cycle, Electron Transport Chain

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Enzymes are made of

back 12

proteins

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Holoenzyme

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An enzyme complete with its apoenzyme and cofactors

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Apoenzyme

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The protein part of an enzyme as opposed to the nonprotein or inorganic cofactors

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Cofactor

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An enzyme accessory. It can be organic, such as coenzymes, or inorganic, such as Fe2+, Mn2+, Zn2+, or other metallic ions.

front 16

Coenzyme

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A complex organic molecule, several of which are derived from vitamins (e.g., nicotinamide or niacin, riboflavin). A coenzyme operates in conjunction with an enzyme. Coenzymes serve as transient carriers of specific atoms or functional groups during metabolic reactions.

front 17

Nucleotide Analogs definition

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a compound that is structurally similar to a nucleotide, which is a building block of DNA and RNA. Nucleotide analogs are used as anti-cancer and anti-viral drugs.

"fake nucleotides"

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Nucleotide analogs (examples)

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Acyclovir, Ribavirin, Azidothymidine

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What can Acyclovir combat?

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Herpesviruses- herpes simplex virus infections , varicella zoster (chickenpox), and herpes zoster (shingles)

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What can Ribavirin combat?

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Respiratory syncytial virus (RSV), hemorrhagic fevers

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What can Azidothymidine combat?

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HIV

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Strategies for Suspecting Drug Resistance and Identifying Drug Resistant Pathogens

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Clinical Observation, In Vitro studies, gene copy number analysis, gene sequence comparison

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In vitro vs in vivo

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In vitro- in a test tube

In vivo- in the host

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Purines

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Adenine, guanine

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Pyrimidines

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Cytosine, thymine, uracil

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Neutrophils

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The most common WBC, they have multilobed nuclei and are very phagocytic

front 27

Cytochromes

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The redox carriers of the electron transport chain that have a tightly bound metal atom responsible for accepting and donating electrons are