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LAB Exam: BIO MO2A

front 1

independent variable

back 1

a variable (often denoted by x) whose variation does not depend on that of another; aka the experimental condition

front 2

dependent variable

back 2

a variable (often denoted by y) whose value depends on that of another. Also called response variable; aka what is being measured

front 3

pH scale

back 3

measures how acidic or basic a solution is; ranges from 0 to 14

front 4

acidic (pH scale)

back 4

any pH value below 7; a substance that releases or causes the release of hydrogen ions (H+) into a solution

front 5

base (pH scale)

back 5

any pH value above 7; a substances that removes H+ from a solution

front 6

Red Cabbage Indicator

back 6

made of anthocyanins; is a pH indicator

- the color of the cabbage extract depends on the Ph of the solution

front 7

Quiz: Interpreting results for Red Cabbage Indicator

match pH w/ color

acidic to basic

back 7

pH 2 = red

pH 4 = pink

pH 6 = purple

pH 7 = violet

pH 8 = blue

pH 10 = grey/green

pH 12 = green

front 8

Anthocyanins

back 8

are pigments responsible for red, blue, and purple colors in flowers, fruits, and autumn leaves. These pigments will change when the pH changes

front 9

Phenol Red indicator

back 9

demonstrates the acidity of hydrochloric acid and the alkalinity of sodium hydroxide; is an indicator dye

front 10

Quiz: interpreting results for Phenol Red

1. Red =

2. pink =

3. Yellow =

back 10

1. Red = neutral;

2. pink = basic;

3. yellow = acidic

front 11

buffer

back 11

is a solution whose pH resists change on addition of small amounts of either an acid or a base; can either by a weak acid w/ a conjugate base or a weak base w/ a conjugate acid

front 12

buffering capacity

back 12

is the amount of a strong acid or base that has to be added to 1 liter of buffer to a cause a PH change of 1.0 pH unit

front 13

positive control

back 13

contains the variable for which you are testing; it will react positively and demonstrate the test's ability to detect what you expect

front 14

negative control

back 14

does not contain the variable for which you are testing; generally contains just the solvent and does not react in the test

Ex: distilled water w/o solute

front 15

Benedict's test

back 15

identifies reducing sugar based on their ability to reduce the cupric (Cu2+) ions to cuprous oxide at basic (high) pH

- cupric oxide is green to reddish orange

front 16

Quiz: interpreting results for Benedict's test

1. green solution

2. reddish organe

3. no color-change

back 16

1. a green solution indicates a small amount of reducing sugar

2. reddish orange indicates an abidance of reducing sugars

3. non-reducing sugars produce n change in color

front 17

Iodine test

back 17

distinguishes starch from monosaccharides, disaccharides, and other polysaccharides

front 18

Quiz: interpreting results for Iodine test

1. bluish black

2. yellowish brown

3. presence of glycogen

back 18

1. positive test! iodine reacts w/ the starch (child polymer of glucose) and becomes bluish black

2. negative test! iodine does not react w/ carbohydrates that are not coiled and remains yellowish brown

3. glycogen has a slightly different structure than does starch and produces only an intermediate color change

front 19

Biuret Test

back 19

a peptide bond forms between the amino group of one amino acid and the carboxyl group of an adjacent amino acid; the reagent is a 1% solution of copper sulfate

- the peptide bond can be identified by this test

front 20

Quiz: interpreting results for Biuret test

1. presence of peptide bonds

2. no peptide bonds

back 20

1. positive result! Peptide bonds (C-N bonds) in protein complex w/ Cu2+ in biuret reagent and produce a violet color

(the intensity of the violet color correlates w/ the amount of peptide bonds present in the test solution)

2. a negative test for proteins is indicated by no color change

front 21

Test for Lipids

back 21

based on a lipid's solubility characteristics in polar solvents, and their ability to produce translucent grease-marks on unglazed paper

front 22

Quiz: interpreting results for Lipid Test

1. liquid at room temperature

2. solid at room temperature

back 22

1. unsaturated fatty acids aka oils; contains double bonds

2. saturated fatty acids; the number of hydrogen atoms attached to the carbon skeleton is maximized

front 23

Amoeba

back 23

are eukaryotes and single-celled animals that catch food and move about by extending fingerlike projections of protoplasm; can either be free-living in damp environments or parasitic

front 24

Blepharisma

back 24

are unicellular ports that are ciliated and found in fresh and salt water

front 25

Trichonympha

back 25

are single-celled protists that are flagellated and commonly found in the gut of termites

front 26

selectively permeable

back 26

a cell membrane that allows only certain items such as water to pass freely through; regulates the movement of other solutes

front 27

diffusion

back 27

can be fedinef as the movement of molecules across a semipermeable membrane from an area of high concentration to an area of low concentration until equilibrium is reached

front 28

osmosis

back 28

is the movement of a solvent across a selectively permeable membrane from a low solute contraction to a region of high solute concentration

front 29

hypotonic

back 29

more solutes inside, so water comes in

- preferred condition of plants (normal)

- animal cells would burst (lysed)

front 30

isotonic

back 30

both sides of the membrane have the same amount of solutes, so no change; solutions are in equilibrium

- good for animal cells

- plants cells are flacid

front 31

hypertonic

back 31

more solutes outside, so water goes out

- animals cells are shriveled

- plants cells are plasmolyzed

front 32

cofactors

back 32

nonprotein substances, bind to the active site on the enzyme and are essential for the enzyme to work

front 33

cellobiase

back 33

an enzyme that breaks down cellulose to glucose by biding the substrate cellobiose to cellobiase to produce glucose molecules

front 34

photosynthesis

back 34

process only used by plants, algae, and cyanobacteria; captures energy from sunlight and converts it into chemical compounds that every organisms uses to power its metabolism (also a source of oxygen necessary for many living organisms)

front 35

photoautotrophs

back 35

organisms that use light to make their own food; include plants, algae, and cyanobacteria

front 36

heterotrophs

back 36

rely on the sugars produced by photosynthetic organisms for their energy needs include animals, fungi, and most bacteria

front 37

equation for photosynthesis

back 37

6CO2 + 6H20 + energy --- C6H12O6 + 602

front 38

spectrophotmeter

back 38

can differentiate which wavelengths of light a substance can absorb; measure transmitted light and compute from it the absorption

front 39

chromatography

back 39

used to separate pigments based on their polarity

- carotenoid is the least polar (was not on the paper at all)

- chlorophyll b was the most polar (was the most prevalent on the paper)

front 40

chlorophyll

back 40

reflect green light waves; found inside the chloroplast; contributes to photosynthesis; may break down in the fall

(absorbs red and blue)

front 41

anthocyaanin

back 41

reflect red or purple light waves; is produced when chlorophyll starts to break down in the fall

front 42

carotenoid

back 42

reflect orange light waves; present in plant cells, but usually overpowered by green chlorophyll; become visible/turn leaves orange when chlorophlll breaks down in the fall

(absorbed blue and green)

front 43

xanthophtyll

back 43

reflect yellow light waves; present in plant cells, but usually overpowered by green chlorophyl; become visible/turn leaves yellow when chlorophlll breaks down in the fall

front 44

Mitosis

back 44

somatic cells only; produces 2 identical daughter cells; NO crossing over; only 1 cycle

front 45

meiosis

back 45

gametes only; produces 4 different daughter cells; crossing over for variation; 2 cycles

front 46

incomplete dominance

back 46

the condition in heterozygote individuals where their phenotype is intermediate between the two homozygous phenotypes

front 47

law of independent assortment

back 47

genes do not influence each other w/ regards to sorting out alleles into gametes; every possible combination of alleles is equally likely to occur

front 48

law of segregation

back 48

paired unit factors (genes) segregate equally into gametes such that offspring have an equal likelihood of inheriting any combination of factors

front 49

phenotype

back 49

the physical expression of the genes possessed by an organism

front 50

genotype

back 50

a listing of the genes of an organism, whether or not the genes are expressed