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Micro Lab Exam 1

front 1

Why are microorganisms hard to see in wet preparations?

back 1

because they move around too quickly and are not stained. They also blend in with their environment.

front 2

Can you distinguish the prokaryotic organisms from the eukaryotic organisms? Explain.

back 2

eukaryotic cells have organelles bound by membranes (i.e. they're compartmentalized) while much of the cellular machinery

prokaryotic cells are floating around freely.

front 3

Why isn’t the oil immersion lens used in the hanging-drop procedure?

back 3

It uses refraction to increase the resolution, therefore, since the hanging-drop procedure has a different refraction index, the oil immersion lens cannot refract the light correctly, due to its incompatibility.

front 4

What is the advantage of using Petri plates rather than test tubes in microbiology?

back 4

Gives room for different colonies to grow

front 5

What are bacteria using for nutrients in nutrient agar?

back 5

Carbon, nitrogen, phosphorus, sulfur, energy

front 6

What is the purpose of the agar?

back 6

To use a solid agent

front 7

How could you determine whether the turbidity in your nutrient broth tube was from a mixture of different microbes or from the growth of only one kind of microbe?

back 7

Transfer the cell sample to a petri dish with agar and see if different types of colonies grow.

front 8

WHAT TWO NAMES DOES SCIENTIFIC NOMENCLATURE ASSIGN?

back 8

GENUS (BEGINS WITH CAPITAL LETTER AND IS ITALICISED)

SPECIES (BEGINS WITH LOWER CASE)

front 9

EUKARYOT INCLUDES ALL OF THE FOLLOWING EXCEPT?

back 9

VIRUSES

front 10

CRYPTOSPORIDIOSIS CAN BE SPREAD IN WATER SUPPLY. WHAT IS IT CAUSED BY?

back 10

PROTOZOA

front 11

WHAT ARE PRIONS?

back 11

INFECTIOUS PRETENTIOUS PROTEINS

front 12

WHAT KIND OF DISEASES DO PRIONS CAUSE?

back 12

MAD COW DISEASE (IN COWS)

SPONGYIFORM ENCEPHALOPATHIES (IN HUMANS) AKA Creutzfeldt–Jakob disease

SCRAPIE (IN SHEEP)

front 13

WHAT TYPE OF IMMUNE CELLS DOES HIV DESTROY?

back 13

CD4 CELLS

front 14

BIOREMEDIATION USES MICROBES TO DO WHAT?

back 14

THEY CLEAN, BREAKDOWN POLLUTANTS & TOXIC WASTE

front 15

WHAT IS THE SMALLEST COMPONENT OF A PURE SUBSTANCE?

back 15

ATOM

front 16

AN ISOTOPE OF OXYGEN HAS AN ATOMIC NUMBER OF 8 AND AN ATOMIC WEIGHT OF 18 HOW MANY NEUTRONS DOES IT HAVE?

back 16

10

front 17

WATER HAS AN UNEQUAL DISTRIBUTION OF CHARGES AND IS CALLED A?

back 17

POLAR MOLECULE

front 18

WHAT TYPE OF CHEMICAL REACTION BREAKS BONDS AND THEN FORMS NEW BONDS?

back 18

EXCHANGE REACTIONS OR REVERSIBLE

front 19

WHY DO SATURATED FATS BECOME SOLID MORE EASILY?

back 19

BECAUSE THE FATTY ACIDS ARE CLOSELY PACKED THERE IS A DOUBLE BOND AND IT CAUSES A KINK IN THE SHAPE.

front 20

WHICH LEVEL OF PROTEIN STRUCTURE REFERS TO OVERALL 3 DEMENTIAL STRUCTURE OF A POLYPEPTIDE CHAIN?

back 20

TERTIARY STRUCTURE

front 21

WHAT IS IN THE PRIMARY STRUCTURE?

back 21

STRAIGHT CHAIN OF AMINO ACIDS

front 22

WHAT IS IN THE SECONDARY STRUCTURE?

back 22

ALPHA HELIX OR BETA HELIX

front 23

WHAT ARE TWO OR MORE FULLY FOLDED PROTEINS INTERACTING WITH EACH OTHER CALLED?

back 23

QUATERNARY STRUCTURE

front 24

WHAT IS THE FUNCTION OF DIAPHRAM ON A COMPOUND MICROSCOPE?

back 24

CONTROLS THE AMOUNT OF LIGHT ENTERING THE CONDENSOR LENSE (LEVER ON THE LIGHT)

front 25

MOST OCULAR LENSES MAGNIFY SPECIMENS BY A FACTOR OF?

back 25

10

front 26

OBJECTIVES SMALLER THAN ABOVE .2MM SUCH AS VIRUS CAN BE VIEWED BY?

back 26

ELECTRON MICROSCOPES

front 27

CAPSULES ARE STAINED BY?

back 27

NEGATIVE STAINING

front 28

BACTERIA THAT DIVIDE AND FORM GRAPELIKE STRUCTURES ARE?

back 28

STAPHYLOCOCCUS

front 29

HOW LONG ARE MOST BACTERIA?

back 29

2-8 MICRONS