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34 notecards = 9 pages (4 cards per page)

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Chapter 1-5, Lab basics

front 1

What quantity of a sample would you need to make a 500uL of a 1/4 dilution

back 1

125u/L

front 2

How many grams of NaCl would you need to make 250mL of a 5% w/v solution?

back 2

12.5

front 3

How many grams of H2SO4 (MW=98) are in 750 mL of 3N H2SO4?

back 3

110.25

front 4

How many microliters in 0.2 mL?

back 4

200

front 5

If the mean of a control is 3.6mg/dL and standard deviation is 0.2 mg/dL, what is the acceptable QC range?

back 5

3.2-4.0 mg/dL

front 6

There are 150g of NaCl per 500 mL solution. (MW = 58.45) What is the molarity?

back 6

5.13 M

front 7

How many grams H2SO4 (MW=98) are in 500 mL of 4N H2SO4 ?

GEW=49

back 7

98 grams

front 8

How many grams of sulfosalicicyclic acid (MW = 254) are required to prepare a 500mL of a 3% (w/v) solution ?

back 8

15 g

front 9

What is the equation for beers law?

back 9

Au/As xCs =Cu

front 10

List two methods used to produce clinical lab grade water?

back 10

Distillation and reverse osmosis

front 11

What 3 items that should be monitored during water purification process

back 11

Resistivity, bacterial contamination, pH

front 12

Identify 3 types of glassware used in the lab

back 12

Borosilicate, low actinic, corex

front 13

Identify four types of plastics used in lab.

back 13

Polypropylene, polyethylene, Teflon, polycarbonate, polystyrene

front 14

What is a TC pipette?

back 14

"Rinse Out" pipettes. Contain exact amount of liquid that must be completely transferred.

front 15

What is TD pipette?

back 15

Designed to drain by gravity, must be held vertically, volume is obtained when draining stops

front 16

TD/blowout is

back 16

Pipette designed after fluid drains must be blown out to get fluid out of tip accurately ( 2 frosted bands)

front 17

What are the six major parts of the spectrophotometer ?

back 17

Light source,entrance slit, monochromator, sample holder, photodetector, amplifier and readout

front 18

What is type 1 water ?

back 18

Purest and is expensive, used in maximum precision testing

front 19

What is type 2 water?

back 19

Use for general lab usage

front 20

What is type 3 water ?

back 20

Used for rinsing of glassware, and media prep, etc.

front 21

Low actinic glassware is

back 21

Amber red in color, contents are visible

front 22

Swing-bucket and fixed angle head rotors are found in?

back 22

Centrifuges

front 23

What is an example of a photodetector?

back 23

A photomultiplier tube

front 24

A release of light energy by a molecule after reacting with a chemical is ______

back 24

Chemiluminescence

front 25

The test menu refers to a list of what???

back 25

Test offered by clinical lab

front 26

OSHA standards are found in what document?

back 26

Codes of federal regulations

front 27

A class A fire involves which of the following?

back 27

Electrical equipment

front 28

Monoclonal antibodies are derived from

back 28

A single cell line

front 29

What is a haptens ?

back 29

A substance that is capable of binding an antibody but can not by themselves stimulate an immune response

front 30

A competitive immunoassay includes an antibody, patient antigen, and ____________

back 30

An antigen with a label attached to it's structure

front 31

Polyclonal gels, particles coated with iron oxide, and plastic beads are all examples of

back 31

A solid phase material

front 32

What immunoassay uses a gold electrode to facilitate production of light ?

back 32

Enzyme multiplied immunoassay technique

front 33

The antibody that binds to hapten-enzyme in the EMIT assay

back 33

Inhibits enzyme activity

front 34

How many milliliters of a 2% solution will make 20 mL of a 10% solution make?

back 34

100 mL

Use C1V1=C2V2