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Microbiology Chapter 5

front 1

How do you know if something is an enzyme?

back 1

It ends in -ase.

front 2

Metabolic pathways are determined by _______.

back 2

Enzymes

front 3

Breaks down complex molecules; provides energy and building blocks for anabolism; exergonic.

back 3

Catabolism

front 4

Uses energy and building blocks to build complex molecules; endergonic.

back 4

Anabolism.

front 5

In general, _______ pathways generate ATP, whereas _______ pathways expend ATP.

back 5

catabolic; anabolic.

front 6

Act on specific substrate and lower the activation energy

back 6

Enzymes

front 7

Substrate contacts the enzyme's active site to form an ____________ complex.

back 7

enzyme-substrate

front 8

The substrate is transformed and rearranged into _______, which are released from the enzyme.

back 8

products.

front 9

Are enzymes specific to particular substrates?

back 9

Yes.

front 10

What is the turnover number (number of substrate molecules an enzyme converts to a product per second)

back 10

Generally 1 to 10,000.

front 11

What is apoenzyme?

back 11

The protein portion of an enzyme.

front 12

What is the cofactor?

back 12

Nonprotein component

front 13

What is the holoenzyme?

back 13

The apoenzyme plus cofactor

front 14

What are some factors that influence enzyme activity?

back 14

Temperature, pH, substrate concentration, inhibitors.

front 15

What happens to an enzyme after it is used?

back 15

Nothing. The enzyme can repeat the same methods over and over again.

front 16

Fills the active site of an enzyme and competes with the substrate

back 16

Competitive inhibitor

front 17

Interacts with another part of the enzyme rather than the active site

back 17

Noncompetitive inhibitor

front 18

RNA that functions as a catalyst by cutting and splicing RNA

back 18

Ribozymes

front 19

During oxidation, is electrons gained or lost?

During reduction, are electrons gained or lost?

back 19

Lost

Gained

OILRIG - Oxidation Is Lost, Reduction Is Gained

front 20

Electrons are transferred from one electron carrier to another along the what?

back 20

Electron transport chain

front 21

What is the only place photophosphorylation occurs?

back 21

Light-trapping photosynthetic cells

front 22

Light energy is converted to ATP when the transfer of electrons (oxidation) from chlorophyll pass through a system of carrier molecules

back 22

Photophosphorylation

front 23

When is ATP and NADPH produced, cyclic or non-cyclic photophosphorylation?

back 23

Non-cyclic.

front 24

The oxidation of glucose to pyruvic acid produces ATP and NADH during what?

back 24

Glycolysis

front 25

How many ATP are using during the beginning stage of Glycolysis?

back 25

2 ATP

front 26

How many ATP are produced during the energy-conserving stage of glycolysis?

back 26

4 ATP; net 2 of ATP.

front 27

How much NAD+ is used during glycolysis?

back 27

2 NAD+

front 28

How much NADH is produced during glycolysis?

back 28

2 NADH

front 29

ATP-generating process in which molecules are oxidized and the final electron acceptor comes from outside the cell and is (almost always) an inorganic molecule.

back 29

Respiration

front 30

What are the two types of respiration?

back 30

Aerobic and anaerobic.

front 31

What is an essential feature of respiration?

back 31

The operation of an electron transport chain.

front 32

In glycolysis, glucose is broken down into what?

back 32

Pyruvic acid

front 33

What is produced during the Krebs cycle?

back 33

ATP, NADH, and FADH 2.

front 34

What are the two most important products obtained during the Krebs cycle?

back 34

NADH and FADH 2

front 35

How much ATP is generated in anaerobic respiration?

back 35

It varies.

front 36

How much ATP is produced during aerobic respiration?

back 36

As much as 38 ATP per glucose molecule.

front 37

Which additional pathway can be taken to get to glycolysis, but does not require glycolysis?

back 37

Entner-Dourdoroff pathway

front 38

How is energy produced in fermentation?

back 38

Glucose is oxidized to form two molecules of pyruvic acid.

front 39

Where do chemoheterotrophs get their energy from?

back 39

Electrons from hydrogen atoms

front 40

Where do photoautotrophs get their energy from?

back 40

Light

front 41

Where do photoheterotrophs get their energy from?

back 41

Light.

front 42

Where do chemoautotrophs get their energy from?

back 42

Electrons from reduced inorganic compounds