19 notecards = 5 pages (4 cards per page)
Hershey & Chase (year and experiment)
dyed phage, found that DNA is genetic material
Watson & Crick (year and experiment)
discover double-stranded antiparallel helix
bc of shape, DNA can act as its own template for replication
how many base pairs per turn of double helix?
A-T has how many H bonds?
G-C has how many H bonds?
semiconservative model of replication
parental DNA splits, one side of each daughter cell is conserved from the parent
DNA polymerase only adds new nucleotides to which end of DNA?
origin of replication
spot on DNA molecule where replication begins
enzymes that untwist the double helix at replication forks
single-strand binding proteins
bind to and stabilize single stranded DNA
enzymes that correct "overwinding" by breaking and rejoining strands
can start RNA chain from scratch
cannot initiate synthesis, can only add nucleotides to existing 3' end
short, 3' end serves as starting point for new DNA strand
joins okazaki fragments
DNA pol III
using parental DNA as template, synthesizes new DNA by adding nucleotides to RNA primer
DNA pol I
removes RNA primer from 5' end and replaces them with DNA nucleotides
synthesized segments of lagging strand
leading strand synthesized from 5' to 3'
lagging strand synthesized in segments added 3' to 5'