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Viewing:

Chapter 3

front 1

The _______ of a compound light microscope focuses light from the light source on the specimen.

back 1

Condenser lens OR condenser

front 2

Which of the following is the most convenient and appropriate unit for expressing the size of an average bacterial cell?

back 2

Micrometer

front 3

All of the following are visible through a compound light microscope except____.

back 3

0.02-AUM ribosome

front 4

Bacterial motility and flagellar movement can readily be viewed using transmission electron microscopy.

back 4

False

front 5

A____________ is equal to 0.000001m.

back 5

micrometer

front 6

Acid-fast Mycobacteria differ from non-acid-fast bacteria by the presence of ____. In the decolorizing step of the acid -fast stain, which reagent is used?

back 6

carbol fuchsin waxy material in their cell wall

front 7

When stained, bacteria in the genus Mycobacterium are ____. When using a 10x ocular lens and a 40x objective, the total magnification achieved is

back 7

acid-fast 400x

front 8

Which of the following is not a modification of a compound microscope?

back 8

electron microscope

front 9

Flagella are too ________ to be seen with the light microscope without adding a mordant.

back 9

small or thin

front 10

Immersion oil acts to decrease refraction of light rays and thus increase resolution.

back 10

True

front 11

In the Gram stain, if the decolorizing step is deleted, gram-negative cells will appear _______ at the completion of the staining procedure.

back 11

Purple

front 12

Magnification is the quality of the microscope that allows on to distinguish between two points that are very close together.

back 12

False

front 13

Place these structures of the compound light microscope in order that light passes through them on the way to the observer's eyes: (1) condenser,(2)ocular lens, (3) illuminator, (4)specimen, (5) objective lens.

back 13

3-1-4-5-2
Response feedback: The path of light begins with the illuminator, then passes through the condenser, the specimen, the objective lens, then then the ocular lens.

front 14

Bacterial smears must be heat-fixed before all staining procedures.

back 14

False

front 15

The ______ charge of a basic dye adheres to the ______ charge of bacterial cell surfaces.

back 15

positive;negative

front 16

The _____ is the part of the microscope that controls the amount of the light entering the condenser.

back 16

diaphragm

front 17

The Gram stain is important in medical microbiology because the results help physicians select appropriate antibiotics for treatment.

back 17

True

front 18

Which of the following kinds of microscopy would be most appropriate for viewing the shape and arrangement of pili or fimbriae on the surface of the bacterial cell?

back 18

scanning electron microscopy

front 19

Which of the following types of microscopy is most useful for viewing the internal structures of unstained specimens?

back 19

Phase-contrast

front 20

You are viewing a sputum smear that has been stained with an acid-fast stain. On this smear you see 5-Aum-long red cells. You can conclude that _______.

back 20

there are acid-fast bacteria in the specimen