front 1 IMViC reactions | back 1 test is 4 in 1 biochemical related tests |
front 2 breakdown/end product of tryptophan | back 2 indole |
front 3 Sugar used as the basis for the methyl red test | back 3 glucose |
front 4 name two media that can be used to detect citrate utilization | back 4 Koser's citrate broth and Simmon's citrate agar |
front 5 medium used to test for indole production | back 5 Tryptone broth |
front 6 medium used to test for methyl red and Voges-Proskauer | back 6 MR-VP broth |
front 7 medium used to test for citrate in this lab | back 7 Simmon's Citrate agar |
front 8 Chemical reagent used in indole test | back 8 Kovac's indole reagent; let stand 10 minutes |
front 9 positive methyl red test pH | back 9 pH below 4.4 |
front 10 name two major end products produced by Voges-Proskauer positive organisms | back 10 2, 3 butanediol and acetonin |
front 11 reagents used in the Voges-Proskauer test | back 11 Barritt's solution A and B |
front 12 members of the Enterobacteriaceae or the enteric bacilli for which the IMViC tests are most useful belong to | back 12 coliform group |
front 13 Enterics that do not fall into the two distinctive categories formed by E. coli and E. aerogenes are collectively referred to as | back 13 intermediates |
front 14 Methyl red test | back 14 tests for fermentation of glucose by looking for acid production |
front 15 when must indole production test be read | back 15 after 24 hour incubation, but no longer! |
front 16 positive indole test | back 16 alcohol in Kovac's reagent separates out and turns red |
front 17 negative indole test | back 17 alcohol in Kovac's reagent remains and color is unchanged |
front 18 Media used in Methyl red test and Voges-Proskauer test | back 18 contains peptone, glucose and a phosphate buffer |
front 19 Methyl red: if glucose has been fermented to acid | back 19 methyl red will be red, below pH of 4.4 |
front 20 Methyl red: if glucose has not been fermented | back 20 methyl red will be yellow, above pH of 4.4 |
front 21 Voges-Proskauer test | back 21 reaction results in red color complex formed by oxidation of 2, 3 butanediol and acetonin and presence of creatine and alpha-napthol |
front 22 Voges-Proskauer: detection of glucose | back 22 red color complex forms if creatine and alpha-napthol are produced; may take 2 hours to develope |
front 23 Citrate utilization | back 23 tests whether an organism can use 6-carbon citrate as its sole source of carbon |
front 24 what must a cell need to carry citrate into cell | back 24 transport protein |
front 25 Citrate test agar | back 25 Simmon's Citrate agar (green colored agar) |
front 26 detection of citrate use | back 26 growth is observed on a slant and is accompanied by a change of the bromothymol blue indicator from green to deep blue |
front 27 positive for citrate use | back 27 color of medium becomes bright blue |
front 28 negative for citrate use | back 28 color stays the same green color |
front 29 why are IMViC tests useful in differentiation? | back 29 differentiates subset of Gram negative, enteric bacteria known as the coliforms; helpful in spotting fecal contamination |
front 30 Indole test | back 30 most E. coli strains produce indole Enterobacter-Klebsiella group does NOT |
front 31 Methyl red test | back 31 most E. coli strains are mixed acid fermenters Enterobacter-Klebsiella group converts fermented glucose into neutral products |
front 32 Voges-Proskauer test | back 32 most E. coli strains produce a negative result (no end products) Enterobacter-Klebsiella group produce a positive result (end products) |
front 33 Citrate test | back 33 E. coli strains cannot use citrate as a carbon source Enterobacter-Klebsiella group can use citrate as a carbon source |