26 notecards = 7 pages (4 cards per page)
Light microscopy refers to the use of any kind of microscope that use visible light to observe specimens
The light microscope, so called because it employs visible light to detect small objects, is probably the most well-known and well-used research tool in biology.
Types of light microscopy
Microscope parts and care
Always carry with 2 hands, one on the arm and one on the base
Only use lens paper for cleaning the lens
Keep liquids away!
Ocular lens (eyepiece)
Remagnifies the image formed by the objective lens
Transmits the image from the objective lens to the ocular lens
Lens that is closest to the slide and provide initial magnification on a specimen (Primary lenses that magnify the specimen)
Holds the microscope slide in position
Focuses light through specimen
Controls the amount of light entering the condenser and the light that reach specimen
Fine focusing knob
Used after initial focusing, to sharpen the image
Coarse focusing knob
Used or initial focusing, should never be used when the high power or oil immersion lens
Magnification by the objective lens X (multiply) Magnification by the Ocular lens
Objective Low Power
view the whole or large portions of specimen
Objective High Power
see small, detailed parts of specimen
Objective Oil Immersion
see very small specimens (e.g., bacteria)
is used to keep light from bending
HOW do the image is formed?
Magnification is due to the RESOLUTION of light as it passes through the lens
the clarity of an image
Light of shorter wavelength produce a clearer image than light of longer wavelength
Best limit o esolution
The best limit of reso;ution (resolving power) for a light microscope is 0.2 Nm ( 200nm)
What position should the stage be when you store the microscope?
at the lowest position
If the resolving power of your microscopy is 250 nm, will you be able to distinguish two point that are 260 nm apart ? why?
Yes because the have a distance apart and it would possible to see it