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Micro Bio Chp 3 Exam 1

front 1

What is the function of the cellular structure indicated by "c" in Figure 3.2?

back 1

provide shape attach to surfaces and protect from dehydration

front 2

Which of the following have a periplasmic space?

back 2

Gram negative bacteria only

front 3

Which of the following prokaryotic cells contain an outer membrane?

back 3

Gram negative bacteria only

front 4

What is the function of the cellular structure indicated by "p" in figure 3-3?

back 4

ATP synthesis

front 5

Viruses are generally measured in ?

back 5

Nanometers

front 6

Which of the following is an INCORRECT pairing?

back 6

numerical aperture; curved glass

front 7

The ability of a lens to gather light is referred to as its

back 7

numerical apertue

front 8

Which of the following are magnifying lenses?

back 8

both objective and the ocular

front 9

If you were trying to visualize flagella without staining, which microscope would you use?

back 9

Phase Contrast

front 10

Why does immersion oil improve resolution?

back 10

It increases numerical aperture and maintains a uniform light speed

front 11

You are shown a micrograph from a light microscope in which the specimens appear bright compared to the background. The micrograph is probably from an ______ microscope.

back 11

Dark field

front 12

The microscope preferred for viewing living specimens is the _____ microscope.

back 12

phase-contrast

front 13

Which of the following is NOT associated with an electron microscope?

back 13

a prism

front 14

All of the following are types of light microscopes EXCEPT

back 14

scanning tunneling

front 15

Which of the following microscopes produces the highest resolution images?

back 15

atomic force

front 16

If a microbiology lab student left the safranin out of the Gram stain procedure, what would be the result?

back 16

Gram positive cells would be purple and Gram negative cells would be colorless

front 17

All of the following are common to both the Gram stain and the acid-fast stain EXCEPT

back 17

a chemical mordant

front 18

Safrainin dye is used as the counterstain in ______ stains.

back 18

Both gram stain and endospore

front 19

Heat is used to drive the stain into cells in the ____ staining procedures.

back 19

both acid-fast and endospore stains

front 20

Carbolfuchsin is the ____ in the acid fast stain.

back 20

primary stain

front 21

A sample is prepared using osminum tetroxide as a stain. This sample has been prepared for an _____ microscope.

back 21

electron

front 22

The kingdoms included in the Linnaeus system of classification are

back 22

Animalia and Plantae

front 23

The rules of naming organisms are called

back 23

nomencalture

front 24

Species and strains of microbes can be distinguished from one another phenotypically using

back 24

serological tests

front 25

Which of the following is NOT a characteristic of a genus name?

back 25

It is usually an adjective

front 26

Carl Woese proposed the concept of the domain based on differences of which of the following cellular molecules?

back 26

ribosomal RNA

front 27

Which of the following classification methods relies on the morphology of organisms?

back 27

physical (phenotypic) characteristics

front 28

A cell's G + C ratio is associated with which of the following classification methods?

back 28

analysis of nucleic acids

front 29

Viruses are not included in the taxonomic scheme proposed by Carl Woese because they lack?

back 29

Ribosomal RNA

front 30

Why have some microbiologist proposed using ribosomal RNA as the basis for defining bacterial species?

back 30

Bacteria are not interbreeding populations, and ribosomal RNAs are highly conserved genes present in all prokaryotes

front 31

Which of the following phenomena produces magnification?

back 31

the refraction of radiation as it passes through a lens

front 32

A virologist wants to observe the surface features of virus particles she is studying. Which of the following microscopes would NOT be useful for her observation?

back 32

differential interference contrast

front 33

A structure that appears in a transmission electron micrograph but is NOT actually present in the specimen is known as a ____

back 33

artifact

front 34

Which of the following statements about transmission electron microscopy is FALSE?

back 34

Glass lenses are used

front 35

Acidic Dyes ___

back 35

are negatively charged and work best at low pH

front 36

A thin film of microbes on a slide is a ____

back 36

smear

front 37

The Gram stain works because of differences in the _____ of bacteria.

back 37

Cell walls

front 38

the primary goal of modern taxonomists is to

back 38

understand the phylogenetic relationships between organisms

front 39

Why are modern light microscopes better than the ones Leeuwenhoek used?

back 39

modern microscopes have a fivefold better resolution

front 40

In the Gram stain procedure, iodine serves as a

back 40

mordant

front 41

What role does safranin play in the Gram stain procedure?

back 41

counterstain

front 42

In Gram staining ethanol-acetone is used as a

back 42

decolorizing agent

front 43

A sample from a patient is prepared using the Gomori methenamine silver stain. What type of microbe is suspected of being present?

back 43

Fungus

front 44

Tungsten is a reagent used in the

back 44

electron microscopy stain

front 45

The ____ stain is one in which eosin is used

back 45

negative

front 46

Tannic acid is a mordant (chemical fictive) used in the ____ stain.

back 46

flagellar

front 47

Methylene blue can be used to stain DNA because it

back 47

forms ionic bonds with DNA

front 48

The _____ stain makes use of malachite green.

back 48

endospore

front 49

The most appropriate unit of measurement for intact archaea is the

back 49

micrometer

front 50

The limits of resolution of atomic force microscopes are in the range of

back 50

nanometers

front 51

the size of a port tapeworm is generally described in terms of

back 51

meters(m)

front 52

A measurement of a mcirobe is reported as 1 x 10 -6m, also known as

back 52

micrometers (um)

front 53

One-thousandths of a meter is a

back 53

millimeter (mm)

front 54

Bacteria and many other microbes do not ______ and therefore do not fit Linneaus' definition species

back 54

reproduce sexually