front 1 What are the following rules for its transport, cleaning, use, and storage of microscope? | back 1 Hold in upright position with one hand on arm, and the other supporting the base. Special, grit-free lens paper is used to clean objective lens and ocular lenses. Use in a circular motion. Always begin focusing process on the lowest power objective lens Use the course adjustment knob only on the lowest power objective lens Always use a coverslip with wet mount preparations. When storing the microscope, always set the lowest power objective lens into position, lower the stage completely, wrap the cord and cover with dust cover. Never remove any parts of the microscope. |
front 2 Define Base of the microscope: | back 2 The foundation support of the microscope. Some have an inclination joints. |
front 3 Substage light or mirror: | back 3 Positioned on the base of the microscope. The substage light passes directly upward through the microscope. If a mirror is used, light must be reflected from a free-standing lamp. |
front 4 Stage: | back 4 The platform the slide rests on while being viewed. Has a hole that permits light to pass through. Spring clips: secure slides on the stage or mechanical stage. |
front 5 Condenser: | back 5 Small substage lens that concentrates the light on the specimen. The condenser may have a rack and pinion knob that raises and lowers the condenser to vary light delivery. |
front 6 Iris diaphragm lever: | back 6 arm attached to the base of the condenser that regulates the amount of light passing through the condenser. Permits the best possible contrast. |
front 7 Course adjustment knob: | back 7 Used to focus the specimen at the lowest-power objective |
front 8 Fine adjustment knob: | back 8 Used for precise focusing once the coarse focus has been completed. |
front 9 Head or body tube: | back 9 Supports the objective lens system which is mounted on a movable nosepiece and the ocular lenses. |
front 10 Arm | back 10 Vertical portion of the microscope connecting the base and head. |
front 11 Ocular (eyepiece) | back 11 Depending on the microscope, one or two, superior end of the head or body tube. Observations are made through the oculars with a magnification of 10X |
front 12 What does magnification of 10X mean? | back 12 Increases the apparent size of the object by 10 times or ten diameters. |
front 13 Where is the pointer attached? | back 13 One of the ocular lenses |
front 14 Nosepiece: | back 14 Rotating mechanism that at the base of the head that the objective lenses are connected to. |
front 15 What is the microscope an instrument of? | back 15 Magnification |
front 16 How is this achieved? | back 16 Through the interplay of 2 lenses, the ocular lens and the objective lens. |
front 17 What is the real image? | back 17 The image produced by the objective lens. |
front 18 What is the virtual image? | back 18 The image produced by the magnification of the ocular lens of the real image. |
front 19 What is the total magnification? | back 19 The magnification of the ocular lens multiplied by the magnification of the objective lens. |
front 20 What is the limitation of the compound microscope? | back 20 Resolution. Objects closer than 0.2um will appear as one object. |
front 21 What does resolution mean? | back 21 The ability to discriminate between two close objects. |
front 22 What is the resolution of the human eye? | back 22 100um |
front 23 What is the resolution of the compound microscope? | back 23 0.2um |
front 24 What determines the resolving power? | back 24 The amount and physical properties of the visible light that enters the microscope. The more light delivered to the objective lens, the greater the resolution. |
front 25 What happens to the size of the objective aperture with increasing magnification? | back 25 It decreases. |
front 26 What is the working distance? | back 26 The distance between the top of the specimen and the bottom of the objective lens when the specimen is in focus. |
front 27 What does parfocal mean? | back 27 That a object should be in focus (or close) at the higher magnifications when in focus at the scanning magnification. |
front 28 What happens to the working distance with increasing magnification? | back 28 It decreases. |
front 29 What is the microscopic field? | back 29 The field of view through the microscope at a given magnification. |
front 30 What is the formula to calculate the microscopic field at said magnification? | back 30 Diameter of field A X magnification A = Diameter of field B X magnification of field B. |
front 31 What is the depth of the field? | back 31 the thickness of the plane that is clearly in focus. |