Print Options

Card layout: ?

← Back to notecard set|Easy Notecards home page

Instructions for Side by Side Printing
  1. Print the notecards
  2. Fold each page in half along the solid vertical line
  3. Cut out the notecards by cutting along each horizontal dotted line
  4. Optional: Glue, tape or staple the ends of each notecard together
  1. Verify Front of pages is selected for Viewing and print the front of the notecards
  2. Select Back of pages for Viewing and print the back of the notecards
    NOTE: Since the back of the pages are printed in reverse order (last page is printed first), keep the pages in the same order as they were after Step 1. Also, be sure to feed the pages in the same direction as you did in Step 1.
  3. Cut out the notecards by cutting along each horizontal and vertical dotted line
To print: Ctrl+PPrint as a list

35 notecards = 9 pages (4 cards per page)

Viewing:

Exercise 3 The Microscope

front 1

back 1

front 2

back 2

front 3

back 3

front 4

back 4

front 5

back 5

front 6

Explain the proper technique for transporting the microscope.

back 6

Hold it in an upright position with one hand on its arm and the other supporting its base.

front 7

The microscope lens may be cleaned with _______.

back 7

grit-free lens paper

front 8

The microscope should be stored with the _______ lens in position over the stage.

back 8

lowest-power objective

front 9

When beginning to focus, use the _______ lens.

back 9

lowest-power

front 10

When focusing, always focus _______ the specimen.

back 10

away from

front 11

A coverslip should always be used with _______.

back 11

wet mounts

front 12

Platform on which the slide rests for viewing

back 12

Stage

front 13

Used to increase the amount of light passing through the specimen

back 13

Iris diaphragm

front 14

Secure(s) the slide to the stage

back 14

Mechanical stage or spring clips

front 15

Delivers a concentrated beam of light to the specimen

back 15

Condenser

front 16

Used for precise focusing once initial focusing had been done

back 16

Fine adjustment knob

front 17

Carries the objective lens; rotates so that the different objective lenses can be brought into position over the specimen

back 17

Nosepiece

front 18

Virtual image

back 18

The real image that is magnified by the ocular lens to produce the virtual image seen by your eye.

front 19

Resolution

back 19

The ability to discriminate two close objects as separate.

front 20

The distance from the bottom of the objective lens to the specimen is called the

back 20

working distance

front 21

Assume there is an object on the left side of the field that you want to bring to the center (that is, toward the apparent right). In what direction would you move your slide?

back 21

to the right

front 22

The area of the specimen seen when looking through the microscope is the

back 22

field

front 23

If a microscope has a 10x ocular and the total magnification at a particular time is 950x, the objective lens in use at the time is _______x.

back 23

95

front 24

Why should the light be dimmed when looking at living (nearly transparent) cells?

back 24

increase contrast

front 25

If, after focusing in low power, only the fine adjustment need be used to focus the specimen at the higher powers, the microscope is said to be _______.

back 25

parfocal

front 26

Is, when using a 10x ocular and a 15x objective, the field size is 1.5mm, the approximate field size with a 30x objective is ____ mm.

back 26

0.75mm

front 27

If the size of the high-power field is 1.2mm, an object occupies approximately third of that field has an estimated diameter of ____ mm.

back 27

0.4mm

front 28

You have been asked to prepare a slide with the letter k on it. Draw the k as seen in the low-power field.

back 28

k (upside down)

front 29

5 mm
2.5mm
0.5mm

back 29

50x
100x
500x

front 30

Say you are observing an object in the low-power field. When you switch to high power, it is no longer in your field of view.
Why might this occur?

back 30

The field decreases proportionately as magnification increases. Therefore, unless the object is
centered at low power, it might be outside the higher-power field

front 31

What should be done initially to prevent this from happening?

back 31

Center the object that you wish to view.

front 32

Increase or decrease as one moves to higher magnifications with the microscope?
1. Resolution
2. Working distance
3. Amount of light needed
4. Depth of field

back 32

1. increases (to a point)
2. decrease
3. increases
4. decreases

front 33

A student has the high-dry lens in position and appears to be intently observing the specimen. The instructor, noting a working distance of about 1 cm, knows the student isn't actually seeing the specimen. How so?

back 33

The working distance for the h.p. lens is closer to 1 mm

front 34

Describe the proper procedure for preparing a wet mount.

back 34

Place the specimen on the slide with a medicine dropper or place a drop of water or saline on the slide. Mix specimen into drop using a toothpick. If staining, add a drop of stain and mix with a toothpick. Hold a coverslip with forceps so that the coverslip touches one side of the specimen drop, and then slowly and carefully lower the angled coverslip onto the specimen.

front 35

Indicate the probable cause of the following situations arising during use of a microscope.
A. Only half of the field is illuminated
B. Field does not change as mechanical stage is moved

back 35

A. The lens is not correctly rotated into place.
B. The slide is not correctly positioned in the clamp on the mechanical stage and does not move when the mechanical stage moves.