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20 notecards = 5 pages (4 cards per page)

Viewing:

Capsule, Endospore, and Flagella Staining

front 1

A Smear for the capsule requires heat-fixing.

back 1

False

front 2

The basic stain (Maneval's) is applied and remains for five minutes.

back 2

False

front 3

What is the purpose for the Congo red stain?

back 3

It stains the background.

front 4

What is the purpose of the Maneval's stain?

back 4

It colorizes the cell proper

front 5

Nigrosin can be used in place of Congo red.

back 5

True

front 6

This stain is both a structural and differential stain.

back 6

True

front 7

Identify 3 species of bacteria that may have capsules.

back 7

Bacillus anthracis, streptococcus pneumoniae, Klebsiella pneumoniae

front 8

The rinsing agent in the Schaeffer-Fultron Method of endospore staining is

back 8

DI water

front 9

The stain is both a structural and differential stain

back 9

True

front 10

The primary stain in the endospore stain is:

back 10

malachite green

front 11

What color are the vegetative cells in the endospore stain?

back 11

red

front 12

Heat is not necessary using the Schaeffer-Fulton method.

back 12

False

front 13

A hood is necessary using the Schaeffer-Fulton method

back 13

True

front 14

Identify 2 genera that have endospores.

back 14

Bacillus and Clostridium

front 15

Match the location of the endospore with the descriptive term.

back 15

Subterminal endospore- The endospore is between the middle and end of the cell.

Terminal endospore- The endospore is on the end of the cell.

Central endospore- The endospore is in the middle of the cell.

front 16

This stain is both a structural and differential stain

back 16

False

front 17

Match the flagella arrangement to the definition:

back 17

Amphitrichous- Flagella at both ends of the bacterium.

Peritrichous- Flagella covering the entire cell surface of the bacterium.

Monotrichous/polar- A single flagellum found on one end of the bacterium.

Lophotrichous- Tufts of flagella at one end of the bacterium.

front 18

Why can't flagella be observed in a living sample?

back 18

Bacterial flagella are too thin to be observed with the light microscope and ordinary stains. They need to be overstained and this kills them unfortunately.

front 19

Organisms with a capsule and endospores are easy to destroy.

back 19

False

front 20

All staining methods used in all the staining exercises performed in lab always color or stain the microbe.

back 20

False