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BCH Exam 3

front 1

A palindrome is

A. A DNA sequence that contains only one kind of base
B. A DNA sequence that contains only two kinds of bases
C. A sequence that reads the same from left to right as from right to left
D. None of the above

back 1

C. A sequence that reads the same from left to right as from right to left

front 2

In the polymerase chain reaction, it is possible to:

A. It is possible to amplify small amounts of DNA without cloning
B. Conditions must be carefully controlled to prevent explosions
C. Reaction mixtures must be chilled at all times
D. All of the above

back 2

A. It is possible to amplify small amounts of DNA without cloning

front 3

Antibiotic resistance genes in vectors are often used as selectable markers in cloning

A. True
B. False

back 3

A. True

front 4

A bacteriophage is

A. A virus that attacks bacteria
B. A piece of DNA derived from two or more sources
C. A small, circular piece of DNA that is not part of the bacterial chromosome
D. An artificially created cytoplasm

back 4

A. A virus that attacks bacteria

front 5

Which of the following statements regarding restriction endoculeases is TRUE?

A. They attack RNA, not DNA
B. They can produce "sticky ends"
C. They can attack one single stranded DNA sequences only
D. They do not display sequence specificity in their site of attack

back 5

B. They can produce "sticky ends"

front 6

The following item was the most important scientific discovery for the development of PCR as a commercially successful and widely-used procedure

A. Taq polymerase
B. Heat-resistant DNA
C. Heat-resistant primers for DNA synthesis
D. Robotic machines to run the PCR procedure
E. Heat-resistant nucleoside triphosphate substrates

back 6

A. Taq polymerase

front 7

In recombinant DNA technology

A. Vectors are used as carriers for recombinant genes
B. It is possible to insert eukaryotic genes into prokaryotic DNA
C. Foreign DNA is frequently inserted into a bacterial plasmid
D. All of these

back 7

D. All of these

front 8

The natural function of restriction endonucleases is to

A. Protect bacterial cells from invasion by viruses (bacteriophages)
B. Help bacteriophages infect cells
C. Regulate gene expression from specific promoters
D. Remove chromatic from histones

back 8

A. Protect bacterial cells from invasion by viruses (bacteriophages)

front 9

Which is the dominant form of DNA found in the cell?

A. A
B. B
C. Z
D. H

back 9

B. B

front 10

Ethidium Bromide slips between the bases of DNA giving it fluorescent properties that differ from those observed when it is free in solution

A. True
B. False

back 10

A. True

front 11

The Z form of DNA

A. Does not exist in nature
B. Is right-handed
C. Tends to occur in purine-only sequences
D. Tends to occur in pyrimidine-only sequences
E. Tends to occur in alternating purine-pyrimidine sequences

back 11

E. Tends to occur in alternating purine-pyrimidine sequences

front 12

Histones are proteins that

A. Are frequently associated with eukaryotic DNA
B. Are frequently associated with prokaryotic DNA
C. Are never found in association with DNA
D. Contain a high percentage of residues with carboxylic acid side chains

back 12

A. Are frequently associated with eukaryotic DNA

front 13

Which of the following nucleobases is a purine?

A. Adenine
B. Cytosine
C. Thymine
D. Uracil

back 13

A. Adenine

front 14

Which of the following bases is NOT found in RNA?

A. A
B. C
C. G
D. T
E. All of these are found in RNA

back 14

D. T

front 15

The outside diameter of a piece of DNA is closest to

A. 2A
B. 20A
C. 200A
D. 2000A

back 15

B. 20A

front 16

What distinguishes nucleotides from nucleosides?

A. Nucleosides lack the phosphate group
B. Nucleosides lack the sugar group
C. Nucleosides lack a nitrogenous base
D. None of the above

back 16

A. Nucleosides lack the phosphate group

front 17

The double helix of DNA is which level of structure?

A. Primary
B. Secondary
C. Tertiary
D. Quaternary

back 17

B. Secondary

front 18

Which of the following groups is NOT present in a cerebroside?

A. Sphingosine
B. Fatty acid
C. Phosphate
D. Sugar

back 18

C. Phosphate

front 19

Unsaturated fatty acids usually have ____ double bonds?

A. Cis
B. Trans

back 19

A. Cis

front 20

How many hydroxyl groups does a molecule of glycerol have?

A. 1
B. 2
C. 3
D. 4

back 20

C. 3

front 21

What characteristics is used most often to define lipids?

A. Ionic Charge
B. Melting Point
C. Solubility
D. Ability to bind metal ions

back 21

C. Solubility

front 22

Which of the following is a metabolic precursor of prostaglandins and leukotrienes?

A. Vitamin A
B. Arachidonic Acid
C. Sphingomyelin
D. Cholesterol

back 22

B. Arachidonic Acid

front 23

The myelin sheaths of neurons typically contain large amounts of

A. Waxes
B. Triacylglycerols
C. Cholesterol
D. Sphingolipids

back 23

D. Sphingolipids

front 24

Which of the following is NOT a product of saponification of a triglyceride?

A. Long chain fatty alcohols
B. The salt of the fatty acids
C. Soaps
D. Glycerol
E. All of these are products of saponification

back 24

A. Long chain fatty alcohols

front 25

What does amphiphatic mean?

A. Having both positive and negative charges
B. Having both acid and base properties
C. Having both hydrophilic and hydrophobic regions
D. Having two stereoisomers

back 25

C. Having both hydrophilic and hydrophobic regions

front 26

Which of the following groups is NOT present in sphingomyelin?

A. Sphingosine
B. Fatty acid
C. Phosphate
D. Sugar

back 26

D. Sugar

front 27

Biological waxes are composed of:

A. Glycerol and only one fatty acid
B. Glycerol and two fatty acids
C. A fatty acid and a long chain alcohol
D. Cholesterol and a fatty acid

back 27

C. A fatty acid and a long chain alcohol

front 28

Base pairs with a propeller twists

A. Are found only in Z DNA
B. Have optimum base stacking
C. Bind water in the minor groove
D. Have stronger hydrogen bonds than base pairs without a propeller twist

back 28

B. Have optimum base stacking

front 29

The structure below is that of

A. Adenine
B. Thymine
C. Cytosine
D. Guanine

back 29

A. Adenine

front 30

Enzymes that seal nicks in DNA are called

A. Restriction enzymes
B. Bacteriophages
C. Ligases
D. Exonucleases

back 30

C. Ligases